Universal Light-Sheet Generation with Field Synthesis

We introduce Field Synthesis, a theorem that can be used to synthesize any scanned or dithered light-sheet, including those used in lattice light-sheet microscopy (LLSM), from an incoherent superposition of one-dimensional intensity distributions. This user-friendly and modular approach offers a dra...

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Detalles Bibliográficos
Autores principales: Chang, Bo-Jui, Kittisopikul, Mark, Dean, Kevin M., Roudot, Philippe, Welf, Erik S., Fiolka, Reto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6561754/
https://www.ncbi.nlm.nih.gov/pubmed/30804550
http://dx.doi.org/10.1038/s41592-019-0327-9
Descripción
Sumario:We introduce Field Synthesis, a theorem that can be used to synthesize any scanned or dithered light-sheet, including those used in lattice light-sheet microscopy (LLSM), from an incoherent superposition of one-dimensional intensity distributions. This user-friendly and modular approach offers a drastically simplified optical design, higher light-throughput, simultaneous multicolor illumination, and a 100% spatial duty cycle that provides biological imaging with low rates of photobleaching.

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