Universal Light-Sheet Generation with Field Synthesis

We introduce Field Synthesis, a theorem that can be used to synthesize any scanned or dithered light-sheet, including those used in lattice light-sheet microscopy (LLSM), from an incoherent superposition of one-dimensional intensity distributions. This user-friendly and modular approach offers a dra...

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Detalles Bibliográficos
Autores principales: Chang, Bo-Jui, Kittisopikul, Mark, Dean, Kevin M., Roudot, Philippe, Welf, Erik S., Fiolka, Reto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6561754/
https://www.ncbi.nlm.nih.gov/pubmed/30804550
http://dx.doi.org/10.1038/s41592-019-0327-9
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author Chang, Bo-Jui
Kittisopikul, Mark
Dean, Kevin M.
Roudot, Philippe
Welf, Erik S.
Fiolka, Reto
author_facet Chang, Bo-Jui
Kittisopikul, Mark
Dean, Kevin M.
Roudot, Philippe
Welf, Erik S.
Fiolka, Reto
author_sort Chang, Bo-Jui
collection PubMed
description We introduce Field Synthesis, a theorem that can be used to synthesize any scanned or dithered light-sheet, including those used in lattice light-sheet microscopy (LLSM), from an incoherent superposition of one-dimensional intensity distributions. This user-friendly and modular approach offers a drastically simplified optical design, higher light-throughput, simultaneous multicolor illumination, and a 100% spatial duty cycle that provides biological imaging with low rates of photobleaching.
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spelling pubmed-65617542019-08-25 Universal Light-Sheet Generation with Field Synthesis Chang, Bo-Jui Kittisopikul, Mark Dean, Kevin M. Roudot, Philippe Welf, Erik S. Fiolka, Reto Nat Methods Article We introduce Field Synthesis, a theorem that can be used to synthesize any scanned or dithered light-sheet, including those used in lattice light-sheet microscopy (LLSM), from an incoherent superposition of one-dimensional intensity distributions. This user-friendly and modular approach offers a drastically simplified optical design, higher light-throughput, simultaneous multicolor illumination, and a 100% spatial duty cycle that provides biological imaging with low rates of photobleaching. 2019-02-25 2019-03 /pmc/articles/PMC6561754/ /pubmed/30804550 http://dx.doi.org/10.1038/s41592-019-0327-9 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Chang, Bo-Jui
Kittisopikul, Mark
Dean, Kevin M.
Roudot, Philippe
Welf, Erik S.
Fiolka, Reto
Universal Light-Sheet Generation with Field Synthesis
title Universal Light-Sheet Generation with Field Synthesis
title_full Universal Light-Sheet Generation with Field Synthesis
title_fullStr Universal Light-Sheet Generation with Field Synthesis
title_full_unstemmed Universal Light-Sheet Generation with Field Synthesis
title_short Universal Light-Sheet Generation with Field Synthesis
title_sort universal light-sheet generation with field synthesis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6561754/
https://www.ncbi.nlm.nih.gov/pubmed/30804550
http://dx.doi.org/10.1038/s41592-019-0327-9
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