Cargando…

Acute Skin Damage and Late Radiation-Induced Fibrosis and Inflammation in Murine Ears after High-Dose Irradiation

The use of different scoring systems for radiation-induced toxicity limits comparability between studies. We examined dose-dependent tissue alterations following hypofractionated X-ray irradiation and evaluated their use as scoring criteria. Four dose fractions (0, 5, 10, 20, 30 Gy/fraction) were ap...

Descripción completa

Detalles Bibliográficos
Autores principales: Dombrowsky, Annique C., Schauer, Jannis, Sammer, Matthias, Blutke, Andreas, Walsh, Dietrich W. M., Schwarz, Benjamin, Bartzsch, Stefan, Feuchtinger, Annette, Reindl, Judith, Combs, Stephanie E., Dollinger, Günther, Schmid, Thomas E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6562452/
https://www.ncbi.nlm.nih.gov/pubmed/31130616
http://dx.doi.org/10.3390/cancers11050727
_version_ 1783426303526961152
author Dombrowsky, Annique C.
Schauer, Jannis
Sammer, Matthias
Blutke, Andreas
Walsh, Dietrich W. M.
Schwarz, Benjamin
Bartzsch, Stefan
Feuchtinger, Annette
Reindl, Judith
Combs, Stephanie E.
Dollinger, Günther
Schmid, Thomas E.
author_facet Dombrowsky, Annique C.
Schauer, Jannis
Sammer, Matthias
Blutke, Andreas
Walsh, Dietrich W. M.
Schwarz, Benjamin
Bartzsch, Stefan
Feuchtinger, Annette
Reindl, Judith
Combs, Stephanie E.
Dollinger, Günther
Schmid, Thomas E.
author_sort Dombrowsky, Annique C.
collection PubMed
description The use of different scoring systems for radiation-induced toxicity limits comparability between studies. We examined dose-dependent tissue alterations following hypofractionated X-ray irradiation and evaluated their use as scoring criteria. Four dose fractions (0, 5, 10, 20, 30 Gy/fraction) were applied daily to ear pinnae. Acute effects (ear thickness, erythema, desquamation) were monitored for 92 days after fraction 1. Late effects (chronic inflammation, fibrosis) and the presence of transforming growth factor beta 1 (TGFβ1)-expressing cells were quantified on day 92. The maximum ear thickness displayed a significant positive correlation with fractional dose. Increased ear thickness and erythema occurred simultaneously, followed by desquamation from day 10 onwards. A significant dose-dependency was observed for the severity of erythema, but not for desquamation. After 4 × 20 and 4 × 30 Gy, inflammation was significantly increased on day 92, whereas fibrosis and the abundance of TGFβ1-expressing cells were only marginally increased after 4 × 30 Gy. Ear thickness significantly correlated with the severity of inflammation and fibrosis on day 92, but not with the number of TGFβ1-expressing cells. Fibrosis correlated significantly with inflammation and fractional dose. In conclusion, the parameter of ear thickness can be used as an objective, numerical and dose-dependent quantification criterion to characterize the severity of acute toxicity and allow for the prediction of late effects.
format Online
Article
Text
id pubmed-6562452
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-65624522019-06-17 Acute Skin Damage and Late Radiation-Induced Fibrosis and Inflammation in Murine Ears after High-Dose Irradiation Dombrowsky, Annique C. Schauer, Jannis Sammer, Matthias Blutke, Andreas Walsh, Dietrich W. M. Schwarz, Benjamin Bartzsch, Stefan Feuchtinger, Annette Reindl, Judith Combs, Stephanie E. Dollinger, Günther Schmid, Thomas E. Cancers (Basel) Article The use of different scoring systems for radiation-induced toxicity limits comparability between studies. We examined dose-dependent tissue alterations following hypofractionated X-ray irradiation and evaluated their use as scoring criteria. Four dose fractions (0, 5, 10, 20, 30 Gy/fraction) were applied daily to ear pinnae. Acute effects (ear thickness, erythema, desquamation) were monitored for 92 days after fraction 1. Late effects (chronic inflammation, fibrosis) and the presence of transforming growth factor beta 1 (TGFβ1)-expressing cells were quantified on day 92. The maximum ear thickness displayed a significant positive correlation with fractional dose. Increased ear thickness and erythema occurred simultaneously, followed by desquamation from day 10 onwards. A significant dose-dependency was observed for the severity of erythema, but not for desquamation. After 4 × 20 and 4 × 30 Gy, inflammation was significantly increased on day 92, whereas fibrosis and the abundance of TGFβ1-expressing cells were only marginally increased after 4 × 30 Gy. Ear thickness significantly correlated with the severity of inflammation and fibrosis on day 92, but not with the number of TGFβ1-expressing cells. Fibrosis correlated significantly with inflammation and fractional dose. In conclusion, the parameter of ear thickness can be used as an objective, numerical and dose-dependent quantification criterion to characterize the severity of acute toxicity and allow for the prediction of late effects. MDPI 2019-05-25 /pmc/articles/PMC6562452/ /pubmed/31130616 http://dx.doi.org/10.3390/cancers11050727 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dombrowsky, Annique C.
Schauer, Jannis
Sammer, Matthias
Blutke, Andreas
Walsh, Dietrich W. M.
Schwarz, Benjamin
Bartzsch, Stefan
Feuchtinger, Annette
Reindl, Judith
Combs, Stephanie E.
Dollinger, Günther
Schmid, Thomas E.
Acute Skin Damage and Late Radiation-Induced Fibrosis and Inflammation in Murine Ears after High-Dose Irradiation
title Acute Skin Damage and Late Radiation-Induced Fibrosis and Inflammation in Murine Ears after High-Dose Irradiation
title_full Acute Skin Damage and Late Radiation-Induced Fibrosis and Inflammation in Murine Ears after High-Dose Irradiation
title_fullStr Acute Skin Damage and Late Radiation-Induced Fibrosis and Inflammation in Murine Ears after High-Dose Irradiation
title_full_unstemmed Acute Skin Damage and Late Radiation-Induced Fibrosis and Inflammation in Murine Ears after High-Dose Irradiation
title_short Acute Skin Damage and Late Radiation-Induced Fibrosis and Inflammation in Murine Ears after High-Dose Irradiation
title_sort acute skin damage and late radiation-induced fibrosis and inflammation in murine ears after high-dose irradiation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6562452/
https://www.ncbi.nlm.nih.gov/pubmed/31130616
http://dx.doi.org/10.3390/cancers11050727
work_keys_str_mv AT dombrowskyanniquec acuteskindamageandlateradiationinducedfibrosisandinflammationinmurineearsafterhighdoseirradiation
AT schauerjannis acuteskindamageandlateradiationinducedfibrosisandinflammationinmurineearsafterhighdoseirradiation
AT sammermatthias acuteskindamageandlateradiationinducedfibrosisandinflammationinmurineearsafterhighdoseirradiation
AT blutkeandreas acuteskindamageandlateradiationinducedfibrosisandinflammationinmurineearsafterhighdoseirradiation
AT walshdietrichwm acuteskindamageandlateradiationinducedfibrosisandinflammationinmurineearsafterhighdoseirradiation
AT schwarzbenjamin acuteskindamageandlateradiationinducedfibrosisandinflammationinmurineearsafterhighdoseirradiation
AT bartzschstefan acuteskindamageandlateradiationinducedfibrosisandinflammationinmurineearsafterhighdoseirradiation
AT feuchtingerannette acuteskindamageandlateradiationinducedfibrosisandinflammationinmurineearsafterhighdoseirradiation
AT reindljudith acuteskindamageandlateradiationinducedfibrosisandinflammationinmurineearsafterhighdoseirradiation
AT combsstephaniee acuteskindamageandlateradiationinducedfibrosisandinflammationinmurineearsafterhighdoseirradiation
AT dollingergunther acuteskindamageandlateradiationinducedfibrosisandinflammationinmurineearsafterhighdoseirradiation
AT schmidthomase acuteskindamageandlateradiationinducedfibrosisandinflammationinmurineearsafterhighdoseirradiation