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Functional Research on Three Presumed Asparagine Synthetase Family Members in Poplar

Asparagine synthetase (AS), a key enzyme in plant nitrogen metabolism, plays an important role in plant nitrogen assimilation and distribution. Asparagine (Asn), the product of asparagine synthetase, is one of the main compounds responsible for organic nitrogen transport and storage in plants. In th...

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Autores principales: Qu, Chunpu, Hao, Bingqing, Xu, Xiuyue, Wang, Yuchen, Yang, Chengjun, Xu, Zhiru, Liu, Guanjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6562506/
https://www.ncbi.nlm.nih.gov/pubmed/31035411
http://dx.doi.org/10.3390/genes10050326
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author Qu, Chunpu
Hao, Bingqing
Xu, Xiuyue
Wang, Yuchen
Yang, Chengjun
Xu, Zhiru
Liu, Guanjun
author_facet Qu, Chunpu
Hao, Bingqing
Xu, Xiuyue
Wang, Yuchen
Yang, Chengjun
Xu, Zhiru
Liu, Guanjun
author_sort Qu, Chunpu
collection PubMed
description Asparagine synthetase (AS), a key enzyme in plant nitrogen metabolism, plays an important role in plant nitrogen assimilation and distribution. Asparagine (Asn), the product of asparagine synthetase, is one of the main compounds responsible for organic nitrogen transport and storage in plants. In this study, we performed complementation experiments using an Asn-deficient Escherichia coli strain to demonstrate that three putative asparagine synthetase family members in poplar (Populus simonii × P. nigra) function in Asn synthesis. Quantitative real-time PCR revealed that the three members had high expression levels in different tissues of poplar and were regulated by exogenous nitrogen. PnAS1 and PnAS2 were also affected by diurnal rhythm. Long-term dark treatment resulted in a significant increase in PnAS1 and PnAS3 expression levels. Under long-term light conditions, however, PnAS2 expression decreased significantly in the intermediate region of leaves. Exogenous application of ammonium nitrogen, glutamine, and a glutamine synthetase inhibitor revealed that PnAS3 was more sensitive to exogenous glutamine, while PnAS1 and PnAS2 were more susceptible to exogenous ammonium nitrogen. Our results suggest that the various members of the PnAS gene family have distinct roles in different tissues and are regulated in different ways.
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spelling pubmed-65625062019-06-17 Functional Research on Three Presumed Asparagine Synthetase Family Members in Poplar Qu, Chunpu Hao, Bingqing Xu, Xiuyue Wang, Yuchen Yang, Chengjun Xu, Zhiru Liu, Guanjun Genes (Basel) Article Asparagine synthetase (AS), a key enzyme in plant nitrogen metabolism, plays an important role in plant nitrogen assimilation and distribution. Asparagine (Asn), the product of asparagine synthetase, is one of the main compounds responsible for organic nitrogen transport and storage in plants. In this study, we performed complementation experiments using an Asn-deficient Escherichia coli strain to demonstrate that three putative asparagine synthetase family members in poplar (Populus simonii × P. nigra) function in Asn synthesis. Quantitative real-time PCR revealed that the three members had high expression levels in different tissues of poplar and were regulated by exogenous nitrogen. PnAS1 and PnAS2 were also affected by diurnal rhythm. Long-term dark treatment resulted in a significant increase in PnAS1 and PnAS3 expression levels. Under long-term light conditions, however, PnAS2 expression decreased significantly in the intermediate region of leaves. Exogenous application of ammonium nitrogen, glutamine, and a glutamine synthetase inhibitor revealed that PnAS3 was more sensitive to exogenous glutamine, while PnAS1 and PnAS2 were more susceptible to exogenous ammonium nitrogen. Our results suggest that the various members of the PnAS gene family have distinct roles in different tissues and are regulated in different ways. MDPI 2019-04-28 /pmc/articles/PMC6562506/ /pubmed/31035411 http://dx.doi.org/10.3390/genes10050326 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Qu, Chunpu
Hao, Bingqing
Xu, Xiuyue
Wang, Yuchen
Yang, Chengjun
Xu, Zhiru
Liu, Guanjun
Functional Research on Three Presumed Asparagine Synthetase Family Members in Poplar
title Functional Research on Three Presumed Asparagine Synthetase Family Members in Poplar
title_full Functional Research on Three Presumed Asparagine Synthetase Family Members in Poplar
title_fullStr Functional Research on Three Presumed Asparagine Synthetase Family Members in Poplar
title_full_unstemmed Functional Research on Three Presumed Asparagine Synthetase Family Members in Poplar
title_short Functional Research on Three Presumed Asparagine Synthetase Family Members in Poplar
title_sort functional research on three presumed asparagine synthetase family members in poplar
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6562506/
https://www.ncbi.nlm.nih.gov/pubmed/31035411
http://dx.doi.org/10.3390/genes10050326
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