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Heat Stress-Responsive Transcriptome Analysis in the Liver Tissue of Hu Sheep
Heat stress has a severe effect on animal health and can reduce the productivity and reproductive efficiency; it is therefore necessary to explore the molecular mechanism involved in heat stress response, which is helpful for the cultivation of an animal breed with resistance to heat stress. However...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6562622/ https://www.ncbi.nlm.nih.gov/pubmed/31121974 http://dx.doi.org/10.3390/genes10050395 |
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author | Li, Yaokun Kong, Lingxuan Deng, Ming Lian, Zhiquan Han, Yinru Sun, Baoli Guo, Yongqing Liu, Guangbin Liu, Dewu |
author_facet | Li, Yaokun Kong, Lingxuan Deng, Ming Lian, Zhiquan Han, Yinru Sun, Baoli Guo, Yongqing Liu, Guangbin Liu, Dewu |
author_sort | Li, Yaokun |
collection | PubMed |
description | Heat stress has a severe effect on animal health and can reduce the productivity and reproductive efficiency; it is therefore necessary to explore the molecular mechanism involved in heat stress response, which is helpful for the cultivation of an animal breed with resistance to heat stress. However, little research about heat stress-responsive molecular analysis has been reported in sheep. Therefore, in this study, RNA sequencing (RNA-Seq) was used to investigate the transcriptome profiling in the liver of Hu sheep with and without heat stress. In total, we detected 520 and 22 differentially expressed mRNAs and lncRNAs, respectively. The differentially expressed mRNAs were mainly associated with metabolic processes, the regulation of biosynthetic processes, and the regulation of glucocorticoid; additionally, they were significantly enriched in the heat stress related pathways, including the carbon metabolism, the PPAR signaling pathway, and vitamin digestion and absorption. The co-located differentially expressed lncRNA Lnc_001782 might positively influence the expression of the corresponding genes APOA4 and APOA5, exerting co-regulative effects on the liver function. Thus, we made the hypothesis that Lnc_001782, APOA4 and APOA5 might function synergistically to regulate the anti-heat stress ability in Hu sheep. This study provides a catalog of Hu sheep liver mRNAs and lncRNAs, and will contribute to a better understanding of the molecular mechanism underlying heat stress responses. |
format | Online Article Text |
id | pubmed-6562622 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-65626222019-06-17 Heat Stress-Responsive Transcriptome Analysis in the Liver Tissue of Hu Sheep Li, Yaokun Kong, Lingxuan Deng, Ming Lian, Zhiquan Han, Yinru Sun, Baoli Guo, Yongqing Liu, Guangbin Liu, Dewu Genes (Basel) Article Heat stress has a severe effect on animal health and can reduce the productivity and reproductive efficiency; it is therefore necessary to explore the molecular mechanism involved in heat stress response, which is helpful for the cultivation of an animal breed with resistance to heat stress. However, little research about heat stress-responsive molecular analysis has been reported in sheep. Therefore, in this study, RNA sequencing (RNA-Seq) was used to investigate the transcriptome profiling in the liver of Hu sheep with and without heat stress. In total, we detected 520 and 22 differentially expressed mRNAs and lncRNAs, respectively. The differentially expressed mRNAs were mainly associated with metabolic processes, the regulation of biosynthetic processes, and the regulation of glucocorticoid; additionally, they were significantly enriched in the heat stress related pathways, including the carbon metabolism, the PPAR signaling pathway, and vitamin digestion and absorption. The co-located differentially expressed lncRNA Lnc_001782 might positively influence the expression of the corresponding genes APOA4 and APOA5, exerting co-regulative effects on the liver function. Thus, we made the hypothesis that Lnc_001782, APOA4 and APOA5 might function synergistically to regulate the anti-heat stress ability in Hu sheep. This study provides a catalog of Hu sheep liver mRNAs and lncRNAs, and will contribute to a better understanding of the molecular mechanism underlying heat stress responses. MDPI 2019-05-22 /pmc/articles/PMC6562622/ /pubmed/31121974 http://dx.doi.org/10.3390/genes10050395 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Li, Yaokun Kong, Lingxuan Deng, Ming Lian, Zhiquan Han, Yinru Sun, Baoli Guo, Yongqing Liu, Guangbin Liu, Dewu Heat Stress-Responsive Transcriptome Analysis in the Liver Tissue of Hu Sheep |
title | Heat Stress-Responsive Transcriptome Analysis in the Liver Tissue of Hu Sheep |
title_full | Heat Stress-Responsive Transcriptome Analysis in the Liver Tissue of Hu Sheep |
title_fullStr | Heat Stress-Responsive Transcriptome Analysis in the Liver Tissue of Hu Sheep |
title_full_unstemmed | Heat Stress-Responsive Transcriptome Analysis in the Liver Tissue of Hu Sheep |
title_short | Heat Stress-Responsive Transcriptome Analysis in the Liver Tissue of Hu Sheep |
title_sort | heat stress-responsive transcriptome analysis in the liver tissue of hu sheep |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6562622/ https://www.ncbi.nlm.nih.gov/pubmed/31121974 http://dx.doi.org/10.3390/genes10050395 |
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