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Prognostic Significance of Serum Free Amino Acids in Head and Neck Cancers

Despite distinctive advances in the field of head and neck squamous cell cancer (HNSCC) biomarker discovery, the spectrum of clinically useful prognostic serum biomarkers is limited. As metabolic activities in highly proliferative transformed cells are fundamentally different from those in non-trans...

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Autores principales: Vsiansky, Vit, Svobodova, Marketa, Gumulec, Jaromir, Cernei, Natalia, Sterbova, Dagmar, Zitka, Ondrej, Kostrica, Rom, Smilek, Pavel, Plzak, Jan, Betka, Jan, Kalfert, David, Masarik, Michal, Raudenska, Martina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6562773/
https://www.ncbi.nlm.nih.gov/pubmed/31075822
http://dx.doi.org/10.3390/cells8050428
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author Vsiansky, Vit
Svobodova, Marketa
Gumulec, Jaromir
Cernei, Natalia
Sterbova, Dagmar
Zitka, Ondrej
Kostrica, Rom
Smilek, Pavel
Plzak, Jan
Betka, Jan
Kalfert, David
Masarik, Michal
Raudenska, Martina
author_facet Vsiansky, Vit
Svobodova, Marketa
Gumulec, Jaromir
Cernei, Natalia
Sterbova, Dagmar
Zitka, Ondrej
Kostrica, Rom
Smilek, Pavel
Plzak, Jan
Betka, Jan
Kalfert, David
Masarik, Michal
Raudenska, Martina
author_sort Vsiansky, Vit
collection PubMed
description Despite distinctive advances in the field of head and neck squamous cell cancer (HNSCC) biomarker discovery, the spectrum of clinically useful prognostic serum biomarkers is limited. As metabolic activities in highly proliferative transformed cells are fundamentally different from those in non-transformed cells, specific shifts in concentration of different metabolites may serve as diagnostic or prognostic markers. Blood amino acids have been identified as promising biomarkers in different cancers before, but little is known about this field in HNSCC. Blood amino acid profiles of 140 HNSCC patients were examined using high-performance liquid chromatography. Cox proportional hazards regression model was used to assess the prognostic value of amino acid concentrations in serum. Colony forming assay was used to identify the effect of amino acids that were significant in Cox proportional hazards regression models on colony forming ability of FaDu and Detroit 562 cell lines. In the multivariable Cox regression model for overall survival (OS), palliative treatment was associated with an unfavourable prognosis while high serum levels of methionine have had a positive prognostic impact. In the relapse-free survival (RFS) multivariable model, methionine was similarly identified as a positive prognostic factor, along with tumor localization in the oropharynx. Oral cavity localization and primary radio(chemo)therapy treatment strategy have been linked to poorer RFS. 1mM serine was shown to support the forming of colonies in both tested HNSCC cell lines. Effect of methionine was exactly the opposite.
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spelling pubmed-65627732019-06-17 Prognostic Significance of Serum Free Amino Acids in Head and Neck Cancers Vsiansky, Vit Svobodova, Marketa Gumulec, Jaromir Cernei, Natalia Sterbova, Dagmar Zitka, Ondrej Kostrica, Rom Smilek, Pavel Plzak, Jan Betka, Jan Kalfert, David Masarik, Michal Raudenska, Martina Cells Article Despite distinctive advances in the field of head and neck squamous cell cancer (HNSCC) biomarker discovery, the spectrum of clinically useful prognostic serum biomarkers is limited. As metabolic activities in highly proliferative transformed cells are fundamentally different from those in non-transformed cells, specific shifts in concentration of different metabolites may serve as diagnostic or prognostic markers. Blood amino acids have been identified as promising biomarkers in different cancers before, but little is known about this field in HNSCC. Blood amino acid profiles of 140 HNSCC patients were examined using high-performance liquid chromatography. Cox proportional hazards regression model was used to assess the prognostic value of amino acid concentrations in serum. Colony forming assay was used to identify the effect of amino acids that were significant in Cox proportional hazards regression models on colony forming ability of FaDu and Detroit 562 cell lines. In the multivariable Cox regression model for overall survival (OS), palliative treatment was associated with an unfavourable prognosis while high serum levels of methionine have had a positive prognostic impact. In the relapse-free survival (RFS) multivariable model, methionine was similarly identified as a positive prognostic factor, along with tumor localization in the oropharynx. Oral cavity localization and primary radio(chemo)therapy treatment strategy have been linked to poorer RFS. 1mM serine was shown to support the forming of colonies in both tested HNSCC cell lines. Effect of methionine was exactly the opposite. MDPI 2019-05-09 /pmc/articles/PMC6562773/ /pubmed/31075822 http://dx.doi.org/10.3390/cells8050428 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Vsiansky, Vit
Svobodova, Marketa
Gumulec, Jaromir
Cernei, Natalia
Sterbova, Dagmar
Zitka, Ondrej
Kostrica, Rom
Smilek, Pavel
Plzak, Jan
Betka, Jan
Kalfert, David
Masarik, Michal
Raudenska, Martina
Prognostic Significance of Serum Free Amino Acids in Head and Neck Cancers
title Prognostic Significance of Serum Free Amino Acids in Head and Neck Cancers
title_full Prognostic Significance of Serum Free Amino Acids in Head and Neck Cancers
title_fullStr Prognostic Significance of Serum Free Amino Acids in Head and Neck Cancers
title_full_unstemmed Prognostic Significance of Serum Free Amino Acids in Head and Neck Cancers
title_short Prognostic Significance of Serum Free Amino Acids in Head and Neck Cancers
title_sort prognostic significance of serum free amino acids in head and neck cancers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6562773/
https://www.ncbi.nlm.nih.gov/pubmed/31075822
http://dx.doi.org/10.3390/cells8050428
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