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Impact of Acute Aerobic Exercise on Genome-Wide DNA-Methylation in Natural Killer Cells—A Pilot Study

Natural Killer (NK-) cells reveal a keen reaction to acute bouts of exercise, including changes of epigenetic modifications. So far, exercise-induced alterations in NK-cell DNA-methylation were shown for single genes only. Studies analyzing genome-wide DNA-methylation have used conglomerates like pe...

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Autores principales: Schenk, Alexander, Koliamitra, Christine, Bauer, Claus Jürgen, Schier, Robert, Schweiger, Michal R., Bloch, Wilhelm, Zimmer, Philipp
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6562781/
https://www.ncbi.nlm.nih.gov/pubmed/31109152
http://dx.doi.org/10.3390/genes10050380
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author Schenk, Alexander
Koliamitra, Christine
Bauer, Claus Jürgen
Schier, Robert
Schweiger, Michal R.
Bloch, Wilhelm
Zimmer, Philipp
author_facet Schenk, Alexander
Koliamitra, Christine
Bauer, Claus Jürgen
Schier, Robert
Schweiger, Michal R.
Bloch, Wilhelm
Zimmer, Philipp
author_sort Schenk, Alexander
collection PubMed
description Natural Killer (NK-) cells reveal a keen reaction to acute bouts of exercise, including changes of epigenetic modifications. So far, exercise-induced alterations in NK-cell DNA-methylation were shown for single genes only. Studies analyzing genome-wide DNA-methylation have used conglomerates like peripheral blood mononuclear cells (PBMCs) rather than specific subsets of immune cells. Therefore, the aim of this pilot-study was to generate first insights into the influence of a single bout of exercise on genome-wide DNA-methylation in isolated NK-cells to open the field for such analyses. Five healthy women performed an incremental step test and blood samples were taken before and after exercise. DNA was isolated from magnet bead sorted NK-cells and further analyzed for global DNA-methylation using the Infinium MethylationEPIC BeadChip. DNA-methylation was changed at 33 targets after acute exercise. These targets were annotated to 25 genes. Of the targets, 19 showed decreased and 14 increased methylation. The 25 genes with altered DNA-methylation have different roles in cell regulation and differ in their molecular functions. These data give new insights in the exercise induced regulation of NK-cells. By using isolated NK-cells, exercise induced differences in DNA-methylation could be shown. Whether or not these changes lead to functional adaptions needs to be elucidated.
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spelling pubmed-65627812019-06-17 Impact of Acute Aerobic Exercise on Genome-Wide DNA-Methylation in Natural Killer Cells—A Pilot Study Schenk, Alexander Koliamitra, Christine Bauer, Claus Jürgen Schier, Robert Schweiger, Michal R. Bloch, Wilhelm Zimmer, Philipp Genes (Basel) Concept Paper Natural Killer (NK-) cells reveal a keen reaction to acute bouts of exercise, including changes of epigenetic modifications. So far, exercise-induced alterations in NK-cell DNA-methylation were shown for single genes only. Studies analyzing genome-wide DNA-methylation have used conglomerates like peripheral blood mononuclear cells (PBMCs) rather than specific subsets of immune cells. Therefore, the aim of this pilot-study was to generate first insights into the influence of a single bout of exercise on genome-wide DNA-methylation in isolated NK-cells to open the field for such analyses. Five healthy women performed an incremental step test and blood samples were taken before and after exercise. DNA was isolated from magnet bead sorted NK-cells and further analyzed for global DNA-methylation using the Infinium MethylationEPIC BeadChip. DNA-methylation was changed at 33 targets after acute exercise. These targets were annotated to 25 genes. Of the targets, 19 showed decreased and 14 increased methylation. The 25 genes with altered DNA-methylation have different roles in cell regulation and differ in their molecular functions. These data give new insights in the exercise induced regulation of NK-cells. By using isolated NK-cells, exercise induced differences in DNA-methylation could be shown. Whether or not these changes lead to functional adaptions needs to be elucidated. MDPI 2019-05-19 /pmc/articles/PMC6562781/ /pubmed/31109152 http://dx.doi.org/10.3390/genes10050380 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Concept Paper
Schenk, Alexander
Koliamitra, Christine
Bauer, Claus Jürgen
Schier, Robert
Schweiger, Michal R.
Bloch, Wilhelm
Zimmer, Philipp
Impact of Acute Aerobic Exercise on Genome-Wide DNA-Methylation in Natural Killer Cells—A Pilot Study
title Impact of Acute Aerobic Exercise on Genome-Wide DNA-Methylation in Natural Killer Cells—A Pilot Study
title_full Impact of Acute Aerobic Exercise on Genome-Wide DNA-Methylation in Natural Killer Cells—A Pilot Study
title_fullStr Impact of Acute Aerobic Exercise on Genome-Wide DNA-Methylation in Natural Killer Cells—A Pilot Study
title_full_unstemmed Impact of Acute Aerobic Exercise on Genome-Wide DNA-Methylation in Natural Killer Cells—A Pilot Study
title_short Impact of Acute Aerobic Exercise on Genome-Wide DNA-Methylation in Natural Killer Cells—A Pilot Study
title_sort impact of acute aerobic exercise on genome-wide dna-methylation in natural killer cells—a pilot study
topic Concept Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6562781/
https://www.ncbi.nlm.nih.gov/pubmed/31109152
http://dx.doi.org/10.3390/genes10050380
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