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De Novo Assembly and Comparative Transcriptome Profiling of Anser anser and Anser cygnoides Geese Species’ Embryonic Skin Feather Follicles

Geese feather production and the quality of downy feathers are additional economically important traits in the geese industry. However, little information is available about the molecular mechanisms fundamental to feather formation and the quality of feathers in geese. This study conducted de novo t...

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Autores principales: Sello, Cornelius Tlotliso, Liu, Chang, Sun, Yongfeng, Msuthwana, Petunia, Hu, Jingtao, Sui, Yujian, Chen, Shaokang, Zhou, Yuxuan, Lu, Hongtao, Xu, Chenguang, Sun, Yue, Liu, Jing, Li, Shengyi, Yang, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6562822/
https://www.ncbi.nlm.nih.gov/pubmed/31072014
http://dx.doi.org/10.3390/genes10050351
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author Sello, Cornelius Tlotliso
Liu, Chang
Sun, Yongfeng
Msuthwana, Petunia
Hu, Jingtao
Sui, Yujian
Chen, Shaokang
Zhou, Yuxuan
Lu, Hongtao
Xu, Chenguang
Sun, Yue
Liu, Jing
Li, Shengyi
Yang, Wei
author_facet Sello, Cornelius Tlotliso
Liu, Chang
Sun, Yongfeng
Msuthwana, Petunia
Hu, Jingtao
Sui, Yujian
Chen, Shaokang
Zhou, Yuxuan
Lu, Hongtao
Xu, Chenguang
Sun, Yue
Liu, Jing
Li, Shengyi
Yang, Wei
author_sort Sello, Cornelius Tlotliso
collection PubMed
description Geese feather production and the quality of downy feathers are additional economically important traits in the geese industry. However, little information is available about the molecular mechanisms fundamental to feather formation and the quality of feathers in geese. This study conducted de novo transcriptome sequencing analysis of two related geese species using the Illumina 4000 platform to determine the genes involved in embryonic skin feather follicle development. A total of 165,564,278 for Anser anser and 144,595,262 for Anser cygnoides clean reads were generated, which were further assembled into 77,134 unigenes with an average length of 906 base pairs in Anser anser and 66,041 unigenes with an average length of 922 base pairs in Anser cygnoides. To recognize the potential regulatory roles of differentially expressed genes (DEGs) during geese embryonic skin feather follicle development, the obtained unigenes were annotated to Gene Ontology (GO), Eukaryotic Orthologous Groups (KOG), and Kyoto Encyclopedia of Genes and Genomes (KEGG) for functional analysis. In both species, GO and KOG had shown similar distribution patterns during functional annotation except for KEGG, which showed significant variation in signaling enrichment. Anser asnser was significantly enriched in the calcium signaling pathway, whereas Anser cygnoides was significantly enriched with glycerolipid metabolism. Further analysis indicated that 14,227 gene families were conserved between the species, among which a total of 20,715 specific gene families were identified. Comparative RNA-Seq data analysis may reveal inclusive knowledge to assist in the identification of genetic regulators at a molecular level to improve feather quality production in geese and other poultry species.
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spelling pubmed-65628222019-06-17 De Novo Assembly and Comparative Transcriptome Profiling of Anser anser and Anser cygnoides Geese Species’ Embryonic Skin Feather Follicles Sello, Cornelius Tlotliso Liu, Chang Sun, Yongfeng Msuthwana, Petunia Hu, Jingtao Sui, Yujian Chen, Shaokang Zhou, Yuxuan Lu, Hongtao Xu, Chenguang Sun, Yue Liu, Jing Li, Shengyi Yang, Wei Genes (Basel) Article Geese feather production and the quality of downy feathers are additional economically important traits in the geese industry. However, little information is available about the molecular mechanisms fundamental to feather formation and the quality of feathers in geese. This study conducted de novo transcriptome sequencing analysis of two related geese species using the Illumina 4000 platform to determine the genes involved in embryonic skin feather follicle development. A total of 165,564,278 for Anser anser and 144,595,262 for Anser cygnoides clean reads were generated, which were further assembled into 77,134 unigenes with an average length of 906 base pairs in Anser anser and 66,041 unigenes with an average length of 922 base pairs in Anser cygnoides. To recognize the potential regulatory roles of differentially expressed genes (DEGs) during geese embryonic skin feather follicle development, the obtained unigenes were annotated to Gene Ontology (GO), Eukaryotic Orthologous Groups (KOG), and Kyoto Encyclopedia of Genes and Genomes (KEGG) for functional analysis. In both species, GO and KOG had shown similar distribution patterns during functional annotation except for KEGG, which showed significant variation in signaling enrichment. Anser asnser was significantly enriched in the calcium signaling pathway, whereas Anser cygnoides was significantly enriched with glycerolipid metabolism. Further analysis indicated that 14,227 gene families were conserved between the species, among which a total of 20,715 specific gene families were identified. Comparative RNA-Seq data analysis may reveal inclusive knowledge to assist in the identification of genetic regulators at a molecular level to improve feather quality production in geese and other poultry species. MDPI 2019-05-08 /pmc/articles/PMC6562822/ /pubmed/31072014 http://dx.doi.org/10.3390/genes10050351 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sello, Cornelius Tlotliso
Liu, Chang
Sun, Yongfeng
Msuthwana, Petunia
Hu, Jingtao
Sui, Yujian
Chen, Shaokang
Zhou, Yuxuan
Lu, Hongtao
Xu, Chenguang
Sun, Yue
Liu, Jing
Li, Shengyi
Yang, Wei
De Novo Assembly and Comparative Transcriptome Profiling of Anser anser and Anser cygnoides Geese Species’ Embryonic Skin Feather Follicles
title De Novo Assembly and Comparative Transcriptome Profiling of Anser anser and Anser cygnoides Geese Species’ Embryonic Skin Feather Follicles
title_full De Novo Assembly and Comparative Transcriptome Profiling of Anser anser and Anser cygnoides Geese Species’ Embryonic Skin Feather Follicles
title_fullStr De Novo Assembly and Comparative Transcriptome Profiling of Anser anser and Anser cygnoides Geese Species’ Embryonic Skin Feather Follicles
title_full_unstemmed De Novo Assembly and Comparative Transcriptome Profiling of Anser anser and Anser cygnoides Geese Species’ Embryonic Skin Feather Follicles
title_short De Novo Assembly and Comparative Transcriptome Profiling of Anser anser and Anser cygnoides Geese Species’ Embryonic Skin Feather Follicles
title_sort de novo assembly and comparative transcriptome profiling of anser anser and anser cygnoides geese species’ embryonic skin feather follicles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6562822/
https://www.ncbi.nlm.nih.gov/pubmed/31072014
http://dx.doi.org/10.3390/genes10050351
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