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Improved Antitumor Efficacy of Combined Vaccine Based on the Induced HUVECs and DC-CT26 Against Colorectal Carcinoma

Angiogenesis is essential for the development, growth, and metastasis of solid tumors. Vaccination with viable human umbilical vein endothelial cells (HUVECs) has been used for antitumor angiogenesis. However, the limited immune response induced by HUVECs hinders their clinical application. In the p...

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Autores principales: Zhang, Qiushuang, Xie, Chao, Wang, Dongyu, Yang, Yi, Liu, Hangfan, Liu, Kangdong, Zhao, Jimin, Chen, Xinhuan, Zhang, Xiaoyan, Yang, Wanjing, Li, Xiang, Tian, Fang, Dong, Ziming, Lu, Jing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6562839/
https://www.ncbi.nlm.nih.gov/pubmed/31121964
http://dx.doi.org/10.3390/cells8050494
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author Zhang, Qiushuang
Xie, Chao
Wang, Dongyu
Yang, Yi
Liu, Hangfan
Liu, Kangdong
Zhao, Jimin
Chen, Xinhuan
Zhang, Xiaoyan
Yang, Wanjing
Li, Xiang
Tian, Fang
Dong, Ziming
Lu, Jing
author_facet Zhang, Qiushuang
Xie, Chao
Wang, Dongyu
Yang, Yi
Liu, Hangfan
Liu, Kangdong
Zhao, Jimin
Chen, Xinhuan
Zhang, Xiaoyan
Yang, Wanjing
Li, Xiang
Tian, Fang
Dong, Ziming
Lu, Jing
author_sort Zhang, Qiushuang
collection PubMed
description Angiogenesis is essential for the development, growth, and metastasis of solid tumors. Vaccination with viable human umbilical vein endothelial cells (HUVECs) has been used for antitumor angiogenesis. However, the limited immune response induced by HUVECs hinders their clinical application. In the present study, we found that HUVECs induced by a tumor microenvironment using the supernatant of murine CT26 colorectal cancer cells exerted a better antiangiogenic effect than HUVECs themselves. The inhibitory effect on tumor growth in the induced HUVEC group was significantly better than that of the HUVEC group, and the induced HUVEC group showed a strong inhibition in CD31-positive microvessel density in the tumor tissues. Moreover, the level of anti-induced HUVEC membrane protein antibody in mouse serum was profoundly higher in the induced HUVEC group than in the HUVEC group. Based on this, the antitumor effect of a vaccine with a combination of induced HUVECs and dendritic cell-loading CT26 antigen (DC-CT26) was evaluated. Notably, the microvessel density of tumor specimens was significantly lower in the combined vaccine group than in the control groups. Furthermore, the spleen index, the killing effect of cytotoxic T lymphocytes (CTLs), and the concentration of interferon-γ in the serum were enhanced in the combined vaccine group. Based on these results, the combined vaccine targeting both tumor angiogenesis and tumor cells may be an attractive and effective cancer immunotherapy strategy.
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spelling pubmed-65628392019-06-17 Improved Antitumor Efficacy of Combined Vaccine Based on the Induced HUVECs and DC-CT26 Against Colorectal Carcinoma Zhang, Qiushuang Xie, Chao Wang, Dongyu Yang, Yi Liu, Hangfan Liu, Kangdong Zhao, Jimin Chen, Xinhuan Zhang, Xiaoyan Yang, Wanjing Li, Xiang Tian, Fang Dong, Ziming Lu, Jing Cells Article Angiogenesis is essential for the development, growth, and metastasis of solid tumors. Vaccination with viable human umbilical vein endothelial cells (HUVECs) has been used for antitumor angiogenesis. However, the limited immune response induced by HUVECs hinders their clinical application. In the present study, we found that HUVECs induced by a tumor microenvironment using the supernatant of murine CT26 colorectal cancer cells exerted a better antiangiogenic effect than HUVECs themselves. The inhibitory effect on tumor growth in the induced HUVEC group was significantly better than that of the HUVEC group, and the induced HUVEC group showed a strong inhibition in CD31-positive microvessel density in the tumor tissues. Moreover, the level of anti-induced HUVEC membrane protein antibody in mouse serum was profoundly higher in the induced HUVEC group than in the HUVEC group. Based on this, the antitumor effect of a vaccine with a combination of induced HUVECs and dendritic cell-loading CT26 antigen (DC-CT26) was evaluated. Notably, the microvessel density of tumor specimens was significantly lower in the combined vaccine group than in the control groups. Furthermore, the spleen index, the killing effect of cytotoxic T lymphocytes (CTLs), and the concentration of interferon-γ in the serum were enhanced in the combined vaccine group. Based on these results, the combined vaccine targeting both tumor angiogenesis and tumor cells may be an attractive and effective cancer immunotherapy strategy. MDPI 2019-05-22 /pmc/articles/PMC6562839/ /pubmed/31121964 http://dx.doi.org/10.3390/cells8050494 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Qiushuang
Xie, Chao
Wang, Dongyu
Yang, Yi
Liu, Hangfan
Liu, Kangdong
Zhao, Jimin
Chen, Xinhuan
Zhang, Xiaoyan
Yang, Wanjing
Li, Xiang
Tian, Fang
Dong, Ziming
Lu, Jing
Improved Antitumor Efficacy of Combined Vaccine Based on the Induced HUVECs and DC-CT26 Against Colorectal Carcinoma
title Improved Antitumor Efficacy of Combined Vaccine Based on the Induced HUVECs and DC-CT26 Against Colorectal Carcinoma
title_full Improved Antitumor Efficacy of Combined Vaccine Based on the Induced HUVECs and DC-CT26 Against Colorectal Carcinoma
title_fullStr Improved Antitumor Efficacy of Combined Vaccine Based on the Induced HUVECs and DC-CT26 Against Colorectal Carcinoma
title_full_unstemmed Improved Antitumor Efficacy of Combined Vaccine Based on the Induced HUVECs and DC-CT26 Against Colorectal Carcinoma
title_short Improved Antitumor Efficacy of Combined Vaccine Based on the Induced HUVECs and DC-CT26 Against Colorectal Carcinoma
title_sort improved antitumor efficacy of combined vaccine based on the induced huvecs and dc-ct26 against colorectal carcinoma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6562839/
https://www.ncbi.nlm.nih.gov/pubmed/31121964
http://dx.doi.org/10.3390/cells8050494
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