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First Isolation and Phylogenetic Analyses of Tick-Borne Encephalitis Virus in Lower Saxony, Germany

Tick-borne encephalitis (TBE) is the most important tick-borne arboviral disease in Europe. Presently, the main endemic regions in Germany are located in the southern half of the country. Although recently, sporadic human TBE cases were reported outside of these known endemic regions. The detection...

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Autores principales: Boelke, Mathias, Bestehorn, Malena, Marchwald, Birgit, Kubinski, Mareike, Liebig, Katrin, Glanz, Julien, Schulz, Claudia, Dobler, Gerhard, Monazahian, Masyar, Becker, Stefanie C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6563265/
https://www.ncbi.nlm.nih.gov/pubmed/31117224
http://dx.doi.org/10.3390/v11050462
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author Boelke, Mathias
Bestehorn, Malena
Marchwald, Birgit
Kubinski, Mareike
Liebig, Katrin
Glanz, Julien
Schulz, Claudia
Dobler, Gerhard
Monazahian, Masyar
Becker, Stefanie C.
author_facet Boelke, Mathias
Bestehorn, Malena
Marchwald, Birgit
Kubinski, Mareike
Liebig, Katrin
Glanz, Julien
Schulz, Claudia
Dobler, Gerhard
Monazahian, Masyar
Becker, Stefanie C.
author_sort Boelke, Mathias
collection PubMed
description Tick-borne encephalitis (TBE) is the most important tick-borne arboviral disease in Europe. Presently, the main endemic regions in Germany are located in the southern half of the country. Although recently, sporadic human TBE cases were reported outside of these known endemic regions. The detection and characterization of invading TBE virus (TBEV) strains will considerably facilitate the surveillance and assessment of this important disease. In 2018, ticks were collected by flagging in several locations of the German federal state of Lower Saxony where TBEV-infections in humans (diagnosed clinical TBE disease or detection of TBEV antibodies) were reported previously. Ticks were pooled according to their developmental stage and tested for TBEV-RNA by RT-qPCR. Five of 730 (0.68%) pools from Ixodes spp. ticks collected in the areas of “Rauher Busch” and “Barsinghausen/Mooshuette” were found positive for TBEV-RNA. Phylogenetic analysis of the whole genomes and E gene sequences revealed a close relationship between the two TBEV isolates, which cluster with a TBEV strain from Poland isolated in 1971. This study provides first data on the phylogeny of TBEV in the German federal state of Lower Saxony, outside of the known TBE endemic areas of Germany. Our results support the hypothesis of an east-west invasion of TBEV strains in Western Europe.
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spelling pubmed-65632652019-06-17 First Isolation and Phylogenetic Analyses of Tick-Borne Encephalitis Virus in Lower Saxony, Germany Boelke, Mathias Bestehorn, Malena Marchwald, Birgit Kubinski, Mareike Liebig, Katrin Glanz, Julien Schulz, Claudia Dobler, Gerhard Monazahian, Masyar Becker, Stefanie C. Viruses Communication Tick-borne encephalitis (TBE) is the most important tick-borne arboviral disease in Europe. Presently, the main endemic regions in Germany are located in the southern half of the country. Although recently, sporadic human TBE cases were reported outside of these known endemic regions. The detection and characterization of invading TBE virus (TBEV) strains will considerably facilitate the surveillance and assessment of this important disease. In 2018, ticks were collected by flagging in several locations of the German federal state of Lower Saxony where TBEV-infections in humans (diagnosed clinical TBE disease or detection of TBEV antibodies) were reported previously. Ticks were pooled according to their developmental stage and tested for TBEV-RNA by RT-qPCR. Five of 730 (0.68%) pools from Ixodes spp. ticks collected in the areas of “Rauher Busch” and “Barsinghausen/Mooshuette” were found positive for TBEV-RNA. Phylogenetic analysis of the whole genomes and E gene sequences revealed a close relationship between the two TBEV isolates, which cluster with a TBEV strain from Poland isolated in 1971. This study provides first data on the phylogeny of TBEV in the German federal state of Lower Saxony, outside of the known TBE endemic areas of Germany. Our results support the hypothesis of an east-west invasion of TBEV strains in Western Europe. MDPI 2019-05-21 /pmc/articles/PMC6563265/ /pubmed/31117224 http://dx.doi.org/10.3390/v11050462 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Boelke, Mathias
Bestehorn, Malena
Marchwald, Birgit
Kubinski, Mareike
Liebig, Katrin
Glanz, Julien
Schulz, Claudia
Dobler, Gerhard
Monazahian, Masyar
Becker, Stefanie C.
First Isolation and Phylogenetic Analyses of Tick-Borne Encephalitis Virus in Lower Saxony, Germany
title First Isolation and Phylogenetic Analyses of Tick-Borne Encephalitis Virus in Lower Saxony, Germany
title_full First Isolation and Phylogenetic Analyses of Tick-Borne Encephalitis Virus in Lower Saxony, Germany
title_fullStr First Isolation and Phylogenetic Analyses of Tick-Borne Encephalitis Virus in Lower Saxony, Germany
title_full_unstemmed First Isolation and Phylogenetic Analyses of Tick-Borne Encephalitis Virus in Lower Saxony, Germany
title_short First Isolation and Phylogenetic Analyses of Tick-Borne Encephalitis Virus in Lower Saxony, Germany
title_sort first isolation and phylogenetic analyses of tick-borne encephalitis virus in lower saxony, germany
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6563265/
https://www.ncbi.nlm.nih.gov/pubmed/31117224
http://dx.doi.org/10.3390/v11050462
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