Cargando…
Subcellular Duplex DNA and G‐Quadruplex Interaction Profiling of a Hexagonal Pt(II) Metallacycle
Metal‐driven self‐assembly afforded a multitude of fascinating supramolecular coordination complexes (SCCs) with applications as catalysts, host–guest, and stimuli‐responsive systems. However, the interest in the biological applications of SCCs is only starting to emerge and thorough characterizatio...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6563712/ https://www.ncbi.nlm.nih.gov/pubmed/31002438 http://dx.doi.org/10.1002/anie.201900934 |
_version_ | 1783426594420817920 |
---|---|
author | Domarco, Olaya Kieler, Claudia Pirker, Christine Dinhof, Carina Englinger, Bernhard Reisecker, Johannes M. Timelthaler, Gerald García, Marcos D. Peinador, Carlos Keppler, Bernhard K. Berger, Walter Terenzi, Alessio |
author_facet | Domarco, Olaya Kieler, Claudia Pirker, Christine Dinhof, Carina Englinger, Bernhard Reisecker, Johannes M. Timelthaler, Gerald García, Marcos D. Peinador, Carlos Keppler, Bernhard K. Berger, Walter Terenzi, Alessio |
author_sort | Domarco, Olaya |
collection | PubMed |
description | Metal‐driven self‐assembly afforded a multitude of fascinating supramolecular coordination complexes (SCCs) with applications as catalysts, host–guest, and stimuli‐responsive systems. However, the interest in the biological applications of SCCs is only starting to emerge and thorough characterization of their behavior in biological milieus is still lacking. Herein, we report on the synthesis and detailed in‐cell tracking of a Pt(2)L(2) metallacycle. We show that our hexagonal supramolecule accumulates in cancer cell nuclei, exerting a distinctive blue fluorescence staining of chromatin resistant to UV photobleaching selectively in nucleolar G4‐rich regions. SCC co‐localizes with epitopes of the quadruplex‐specific antibody BG4 and replaces other well‐known G4 stabilizers. Moreover, the photophysical changes accompanying the metallacycle binding to G4s in solution (fluorescence quenching, absorption enhancement) also take place intracellularly, allowing its subcellular interaction tracking. |
format | Online Article Text |
id | pubmed-6563712 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-65637122019-06-20 Subcellular Duplex DNA and G‐Quadruplex Interaction Profiling of a Hexagonal Pt(II) Metallacycle Domarco, Olaya Kieler, Claudia Pirker, Christine Dinhof, Carina Englinger, Bernhard Reisecker, Johannes M. Timelthaler, Gerald García, Marcos D. Peinador, Carlos Keppler, Bernhard K. Berger, Walter Terenzi, Alessio Angew Chem Int Ed Engl Communications Metal‐driven self‐assembly afforded a multitude of fascinating supramolecular coordination complexes (SCCs) with applications as catalysts, host–guest, and stimuli‐responsive systems. However, the interest in the biological applications of SCCs is only starting to emerge and thorough characterization of their behavior in biological milieus is still lacking. Herein, we report on the synthesis and detailed in‐cell tracking of a Pt(2)L(2) metallacycle. We show that our hexagonal supramolecule accumulates in cancer cell nuclei, exerting a distinctive blue fluorescence staining of chromatin resistant to UV photobleaching selectively in nucleolar G4‐rich regions. SCC co‐localizes with epitopes of the quadruplex‐specific antibody BG4 and replaces other well‐known G4 stabilizers. Moreover, the photophysical changes accompanying the metallacycle binding to G4s in solution (fluorescence quenching, absorption enhancement) also take place intracellularly, allowing its subcellular interaction tracking. John Wiley and Sons Inc. 2019-05-08 2019-06-11 /pmc/articles/PMC6563712/ /pubmed/31002438 http://dx.doi.org/10.1002/anie.201900934 Text en © 2019 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
spellingShingle | Communications Domarco, Olaya Kieler, Claudia Pirker, Christine Dinhof, Carina Englinger, Bernhard Reisecker, Johannes M. Timelthaler, Gerald García, Marcos D. Peinador, Carlos Keppler, Bernhard K. Berger, Walter Terenzi, Alessio Subcellular Duplex DNA and G‐Quadruplex Interaction Profiling of a Hexagonal Pt(II) Metallacycle |
title | Subcellular Duplex DNA and G‐Quadruplex Interaction Profiling of a Hexagonal Pt(II) Metallacycle |
title_full | Subcellular Duplex DNA and G‐Quadruplex Interaction Profiling of a Hexagonal Pt(II) Metallacycle |
title_fullStr | Subcellular Duplex DNA and G‐Quadruplex Interaction Profiling of a Hexagonal Pt(II) Metallacycle |
title_full_unstemmed | Subcellular Duplex DNA and G‐Quadruplex Interaction Profiling of a Hexagonal Pt(II) Metallacycle |
title_short | Subcellular Duplex DNA and G‐Quadruplex Interaction Profiling of a Hexagonal Pt(II) Metallacycle |
title_sort | subcellular duplex dna and g‐quadruplex interaction profiling of a hexagonal pt(ii) metallacycle |
topic | Communications |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6563712/ https://www.ncbi.nlm.nih.gov/pubmed/31002438 http://dx.doi.org/10.1002/anie.201900934 |
work_keys_str_mv | AT domarcoolaya subcellularduplexdnaandgquadruplexinteractionprofilingofahexagonalptiimetallacycle AT kielerclaudia subcellularduplexdnaandgquadruplexinteractionprofilingofahexagonalptiimetallacycle AT pirkerchristine subcellularduplexdnaandgquadruplexinteractionprofilingofahexagonalptiimetallacycle AT dinhofcarina subcellularduplexdnaandgquadruplexinteractionprofilingofahexagonalptiimetallacycle AT englingerbernhard subcellularduplexdnaandgquadruplexinteractionprofilingofahexagonalptiimetallacycle AT reiseckerjohannesm subcellularduplexdnaandgquadruplexinteractionprofilingofahexagonalptiimetallacycle AT timelthalergerald subcellularduplexdnaandgquadruplexinteractionprofilingofahexagonalptiimetallacycle AT garciamarcosd subcellularduplexdnaandgquadruplexinteractionprofilingofahexagonalptiimetallacycle AT peinadorcarlos subcellularduplexdnaandgquadruplexinteractionprofilingofahexagonalptiimetallacycle AT kepplerbernhardk subcellularduplexdnaandgquadruplexinteractionprofilingofahexagonalptiimetallacycle AT bergerwalter subcellularduplexdnaandgquadruplexinteractionprofilingofahexagonalptiimetallacycle AT terenzialessio subcellularduplexdnaandgquadruplexinteractionprofilingofahexagonalptiimetallacycle |