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Presence & mobility of antimicrobial resistance in Gram-negative bacteria from environmental samples in coastal Karnataka, India

To understand antimicrobial resistance (AMR) patterns and mechanisms of horizontal gene transfer in human-associated environments is essential to AMR surveillance. Gram-negative bacteria (1122 isolates) from food-animal environments were characterized for antimicrobial susceptibility and AMR genes....

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Autores principales: Mohan Raj, Juliet Roshini, Vittal, Rajeshwari, Shivakumaraswamy, Santhosh Kogaluru, Deekshit, Vijaya Kumar, Chakraborty, Anirban, Karunasagar, Indrani
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6563727/
https://www.ncbi.nlm.nih.gov/pubmed/31219097
http://dx.doi.org/10.4103/ijmr.IJMR_2088_17
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author Mohan Raj, Juliet Roshini
Vittal, Rajeshwari
Shivakumaraswamy, Santhosh Kogaluru
Deekshit, Vijaya Kumar
Chakraborty, Anirban
Karunasagar, Indrani
author_facet Mohan Raj, Juliet Roshini
Vittal, Rajeshwari
Shivakumaraswamy, Santhosh Kogaluru
Deekshit, Vijaya Kumar
Chakraborty, Anirban
Karunasagar, Indrani
author_sort Mohan Raj, Juliet Roshini
collection PubMed
description To understand antimicrobial resistance (AMR) patterns and mechanisms of horizontal gene transfer in human-associated environments is essential to AMR surveillance. Gram-negative bacteria (1122 isolates) from food-animal environments were characterized for antimicrobial susceptibility and AMR genes. Seventy five per cent of the isolates (837 of 1122) were resistant to at least one of the antibiotics tested. Resistance to more than three groups of antimicrobials (multidrug resistance) was observed in 43 isolates with most often encountered (12 of 43) resistance to β-lactams, tetracycline, quinolones and nitrofurantoin. The profile of frequently reported plasmid-mediated resistance gene in these isolates was determined. The mobility of these elements as plasmids or phages was examined. The bla(CTX-M) gene was present in the plasmid of 61 per cent and packed in induced phage fractions in 72 per cent of the isolates and bla(TEM) in 69 per cent phage fractions compared to 15 per cent presence in the plasmid.
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spelling pubmed-65637272019-06-14 Presence & mobility of antimicrobial resistance in Gram-negative bacteria from environmental samples in coastal Karnataka, India Mohan Raj, Juliet Roshini Vittal, Rajeshwari Shivakumaraswamy, Santhosh Kogaluru Deekshit, Vijaya Kumar Chakraborty, Anirban Karunasagar, Indrani Indian J Med Res Original Article To understand antimicrobial resistance (AMR) patterns and mechanisms of horizontal gene transfer in human-associated environments is essential to AMR surveillance. Gram-negative bacteria (1122 isolates) from food-animal environments were characterized for antimicrobial susceptibility and AMR genes. Seventy five per cent of the isolates (837 of 1122) were resistant to at least one of the antibiotics tested. Resistance to more than three groups of antimicrobials (multidrug resistance) was observed in 43 isolates with most often encountered (12 of 43) resistance to β-lactams, tetracycline, quinolones and nitrofurantoin. The profile of frequently reported plasmid-mediated resistance gene in these isolates was determined. The mobility of these elements as plasmids or phages was examined. The bla(CTX-M) gene was present in the plasmid of 61 per cent and packed in induced phage fractions in 72 per cent of the isolates and bla(TEM) in 69 per cent phage fractions compared to 15 per cent presence in the plasmid. Wolters Kluwer - Medknow 2019-02 /pmc/articles/PMC6563727/ /pubmed/31219097 http://dx.doi.org/10.4103/ijmr.IJMR_2088_17 Text en Copyright: © 2019 Indian Journal of Medical Research http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Mohan Raj, Juliet Roshini
Vittal, Rajeshwari
Shivakumaraswamy, Santhosh Kogaluru
Deekshit, Vijaya Kumar
Chakraborty, Anirban
Karunasagar, Indrani
Presence & mobility of antimicrobial resistance in Gram-negative bacteria from environmental samples in coastal Karnataka, India
title Presence & mobility of antimicrobial resistance in Gram-negative bacteria from environmental samples in coastal Karnataka, India
title_full Presence & mobility of antimicrobial resistance in Gram-negative bacteria from environmental samples in coastal Karnataka, India
title_fullStr Presence & mobility of antimicrobial resistance in Gram-negative bacteria from environmental samples in coastal Karnataka, India
title_full_unstemmed Presence & mobility of antimicrobial resistance in Gram-negative bacteria from environmental samples in coastal Karnataka, India
title_short Presence & mobility of antimicrobial resistance in Gram-negative bacteria from environmental samples in coastal Karnataka, India
title_sort presence & mobility of antimicrobial resistance in gram-negative bacteria from environmental samples in coastal karnataka, india
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6563727/
https://www.ncbi.nlm.nih.gov/pubmed/31219097
http://dx.doi.org/10.4103/ijmr.IJMR_2088_17
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