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Genome-wide analysis of the WRKY gene family in drumstick (Moringa oleifera Lam.)

WRKY proteins belong to one of the largest families of transcription factors. They have important functions in plant growth and development, signal transduction and stress responses. However, little information is available regarding the WRKY family in drumstick (Moringa oleifera Lam.). In the prese...

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Autores principales: Zhang, Junjie, Yang, Endian, He, Qian, Lin, Mengfei, Zhou, Wei, Pian, Ruiqi, Chen, Xiaoyang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6563795/
https://www.ncbi.nlm.nih.gov/pubmed/31218125
http://dx.doi.org/10.7717/peerj.7063
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author Zhang, Junjie
Yang, Endian
He, Qian
Lin, Mengfei
Zhou, Wei
Pian, Ruiqi
Chen, Xiaoyang
author_facet Zhang, Junjie
Yang, Endian
He, Qian
Lin, Mengfei
Zhou, Wei
Pian, Ruiqi
Chen, Xiaoyang
author_sort Zhang, Junjie
collection PubMed
description WRKY proteins belong to one of the largest families of transcription factors. They have important functions in plant growth and development, signal transduction and stress responses. However, little information is available regarding the WRKY family in drumstick (Moringa oleifera Lam.). In the present study, we identified 54 MoWRKY genes in this species using genomic data. On the basis of structural features of the proteins they encode, the MoWRKY genes were classified into three main groups, with the second group being further divided into five subgroups. Phylogenetic trees constructed from the sequences of WRKY domains and overall amino acid compositions derived from drumstick and Arabidopsis were similar; the results indicated that the WRKY domain was the main evolutionary unit of WRKY genes. Gene structure and conserved motif analysis showed that genes with similar structures and proteins with similar motif compositions were usually clustered in the same class. Selective pressure analysis indicated that although neutral evolution and positive selection have happened in several MoWRKY genes, most have evolved under strong purifying selection. Moreover, different subgroups had evolved at different rates. The levels of expression of MoWRKY genes in response to five different abiotic stresses (salt, heat, drought, H(2)O(2), cold) were evaluated by reverse transcription polymerase chain reaction (RT-PCR) and quantitative RT-PCR (qRT-PCR), with the results indicating that these genes had different expression levels and that some may be involved in abiotic stress responses. Our results will provide a foundation for cloning genes with specific functions for use in further research and applications.
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spelling pubmed-65637952019-06-19 Genome-wide analysis of the WRKY gene family in drumstick (Moringa oleifera Lam.) Zhang, Junjie Yang, Endian He, Qian Lin, Mengfei Zhou, Wei Pian, Ruiqi Chen, Xiaoyang PeerJ Genetics WRKY proteins belong to one of the largest families of transcription factors. They have important functions in plant growth and development, signal transduction and stress responses. However, little information is available regarding the WRKY family in drumstick (Moringa oleifera Lam.). In the present study, we identified 54 MoWRKY genes in this species using genomic data. On the basis of structural features of the proteins they encode, the MoWRKY genes were classified into three main groups, with the second group being further divided into five subgroups. Phylogenetic trees constructed from the sequences of WRKY domains and overall amino acid compositions derived from drumstick and Arabidopsis were similar; the results indicated that the WRKY domain was the main evolutionary unit of WRKY genes. Gene structure and conserved motif analysis showed that genes with similar structures and proteins with similar motif compositions were usually clustered in the same class. Selective pressure analysis indicated that although neutral evolution and positive selection have happened in several MoWRKY genes, most have evolved under strong purifying selection. Moreover, different subgroups had evolved at different rates. The levels of expression of MoWRKY genes in response to five different abiotic stresses (salt, heat, drought, H(2)O(2), cold) were evaluated by reverse transcription polymerase chain reaction (RT-PCR) and quantitative RT-PCR (qRT-PCR), with the results indicating that these genes had different expression levels and that some may be involved in abiotic stress responses. Our results will provide a foundation for cloning genes with specific functions for use in further research and applications. PeerJ Inc. 2019-06-10 /pmc/articles/PMC6563795/ /pubmed/31218125 http://dx.doi.org/10.7717/peerj.7063 Text en ©2019 Zhang et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Genetics
Zhang, Junjie
Yang, Endian
He, Qian
Lin, Mengfei
Zhou, Wei
Pian, Ruiqi
Chen, Xiaoyang
Genome-wide analysis of the WRKY gene family in drumstick (Moringa oleifera Lam.)
title Genome-wide analysis of the WRKY gene family in drumstick (Moringa oleifera Lam.)
title_full Genome-wide analysis of the WRKY gene family in drumstick (Moringa oleifera Lam.)
title_fullStr Genome-wide analysis of the WRKY gene family in drumstick (Moringa oleifera Lam.)
title_full_unstemmed Genome-wide analysis of the WRKY gene family in drumstick (Moringa oleifera Lam.)
title_short Genome-wide analysis of the WRKY gene family in drumstick (Moringa oleifera Lam.)
title_sort genome-wide analysis of the wrky gene family in drumstick (moringa oleifera lam.)
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6563795/
https://www.ncbi.nlm.nih.gov/pubmed/31218125
http://dx.doi.org/10.7717/peerj.7063
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