An orange calcium-modulated bioluminescent indicator for non-invasive activity imaging

Fluorescent indicators are widely used to visualize calcium dynamics downstream of membrane depolarization or G protein-coupled receptor activation, but are poorly suited for non-invasive imaging in mammals. Here, we report a bright calcium-modulated bioluminescent indicator named Orange CaMBI. Oran...

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Detalles Bibliográficos
Autores principales: Oh, Younghee, Park, Yunhee, Cho, Julia H., Wu, Haodi, Paulk, Nicole K., Liu, Lan Xiang, Kim, Namdoo, Kay, Mark A., Wu, Joseph C., Lin, Michael Z.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6563924/
https://www.ncbi.nlm.nih.gov/pubmed/30936501
http://dx.doi.org/10.1038/s41589-019-0256-z
Descripción
Sumario:Fluorescent indicators are widely used to visualize calcium dynamics downstream of membrane depolarization or G protein-coupled receptor activation, but are poorly suited for non-invasive imaging in mammals. Here, we report a bright calcium-modulated bioluminescent indicator named Orange CaMBI. Orange CaMBI reports calcium dynamics in single cells and, in the context of a transgenic mouse, reveals calcium oscillations in whole organs in an entirely noninvasive manner.

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