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An orange calcium-modulated bioluminescent indicator for non-invasive activity imaging
Fluorescent indicators are widely used to visualize calcium dynamics downstream of membrane depolarization or G protein-coupled receptor activation, but are poorly suited for non-invasive imaging in mammals. Here, we report a bright calcium-modulated bioluminescent indicator named Orange CaMBI. Oran...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6563924/ https://www.ncbi.nlm.nih.gov/pubmed/30936501 http://dx.doi.org/10.1038/s41589-019-0256-z |
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author | Oh, Younghee Park, Yunhee Cho, Julia H. Wu, Haodi Paulk, Nicole K. Liu, Lan Xiang Kim, Namdoo Kay, Mark A. Wu, Joseph C. Lin, Michael Z. |
author_facet | Oh, Younghee Park, Yunhee Cho, Julia H. Wu, Haodi Paulk, Nicole K. Liu, Lan Xiang Kim, Namdoo Kay, Mark A. Wu, Joseph C. Lin, Michael Z. |
author_sort | Oh, Younghee |
collection | PubMed |
description | Fluorescent indicators are widely used to visualize calcium dynamics downstream of membrane depolarization or G protein-coupled receptor activation, but are poorly suited for non-invasive imaging in mammals. Here, we report a bright calcium-modulated bioluminescent indicator named Orange CaMBI. Orange CaMBI reports calcium dynamics in single cells and, in the context of a transgenic mouse, reveals calcium oscillations in whole organs in an entirely noninvasive manner. |
format | Online Article Text |
id | pubmed-6563924 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
record_format | MEDLINE/PubMed |
spelling | pubmed-65639242019-10-01 An orange calcium-modulated bioluminescent indicator for non-invasive activity imaging Oh, Younghee Park, Yunhee Cho, Julia H. Wu, Haodi Paulk, Nicole K. Liu, Lan Xiang Kim, Namdoo Kay, Mark A. Wu, Joseph C. Lin, Michael Z. Nat Chem Biol Article Fluorescent indicators are widely used to visualize calcium dynamics downstream of membrane depolarization or G protein-coupled receptor activation, but are poorly suited for non-invasive imaging in mammals. Here, we report a bright calcium-modulated bioluminescent indicator named Orange CaMBI. Orange CaMBI reports calcium dynamics in single cells and, in the context of a transgenic mouse, reveals calcium oscillations in whole organs in an entirely noninvasive manner. 2019-04-01 2019-05 /pmc/articles/PMC6563924/ /pubmed/30936501 http://dx.doi.org/10.1038/s41589-019-0256-z Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Oh, Younghee Park, Yunhee Cho, Julia H. Wu, Haodi Paulk, Nicole K. Liu, Lan Xiang Kim, Namdoo Kay, Mark A. Wu, Joseph C. Lin, Michael Z. An orange calcium-modulated bioluminescent indicator for non-invasive activity imaging |
title | An orange calcium-modulated bioluminescent indicator for non-invasive activity imaging |
title_full | An orange calcium-modulated bioluminescent indicator for non-invasive activity imaging |
title_fullStr | An orange calcium-modulated bioluminescent indicator for non-invasive activity imaging |
title_full_unstemmed | An orange calcium-modulated bioluminescent indicator for non-invasive activity imaging |
title_short | An orange calcium-modulated bioluminescent indicator for non-invasive activity imaging |
title_sort | orange calcium-modulated bioluminescent indicator for non-invasive activity imaging |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6563924/ https://www.ncbi.nlm.nih.gov/pubmed/30936501 http://dx.doi.org/10.1038/s41589-019-0256-z |
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