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An orange calcium-modulated bioluminescent indicator for non-invasive activity imaging

Fluorescent indicators are widely used to visualize calcium dynamics downstream of membrane depolarization or G protein-coupled receptor activation, but are poorly suited for non-invasive imaging in mammals. Here, we report a bright calcium-modulated bioluminescent indicator named Orange CaMBI. Oran...

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Autores principales: Oh, Younghee, Park, Yunhee, Cho, Julia H., Wu, Haodi, Paulk, Nicole K., Liu, Lan Xiang, Kim, Namdoo, Kay, Mark A., Wu, Joseph C., Lin, Michael Z.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6563924/
https://www.ncbi.nlm.nih.gov/pubmed/30936501
http://dx.doi.org/10.1038/s41589-019-0256-z
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author Oh, Younghee
Park, Yunhee
Cho, Julia H.
Wu, Haodi
Paulk, Nicole K.
Liu, Lan Xiang
Kim, Namdoo
Kay, Mark A.
Wu, Joseph C.
Lin, Michael Z.
author_facet Oh, Younghee
Park, Yunhee
Cho, Julia H.
Wu, Haodi
Paulk, Nicole K.
Liu, Lan Xiang
Kim, Namdoo
Kay, Mark A.
Wu, Joseph C.
Lin, Michael Z.
author_sort Oh, Younghee
collection PubMed
description Fluorescent indicators are widely used to visualize calcium dynamics downstream of membrane depolarization or G protein-coupled receptor activation, but are poorly suited for non-invasive imaging in mammals. Here, we report a bright calcium-modulated bioluminescent indicator named Orange CaMBI. Orange CaMBI reports calcium dynamics in single cells and, in the context of a transgenic mouse, reveals calcium oscillations in whole organs in an entirely noninvasive manner.
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spelling pubmed-65639242019-10-01 An orange calcium-modulated bioluminescent indicator for non-invasive activity imaging Oh, Younghee Park, Yunhee Cho, Julia H. Wu, Haodi Paulk, Nicole K. Liu, Lan Xiang Kim, Namdoo Kay, Mark A. Wu, Joseph C. Lin, Michael Z. Nat Chem Biol Article Fluorescent indicators are widely used to visualize calcium dynamics downstream of membrane depolarization or G protein-coupled receptor activation, but are poorly suited for non-invasive imaging in mammals. Here, we report a bright calcium-modulated bioluminescent indicator named Orange CaMBI. Orange CaMBI reports calcium dynamics in single cells and, in the context of a transgenic mouse, reveals calcium oscillations in whole organs in an entirely noninvasive manner. 2019-04-01 2019-05 /pmc/articles/PMC6563924/ /pubmed/30936501 http://dx.doi.org/10.1038/s41589-019-0256-z Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Oh, Younghee
Park, Yunhee
Cho, Julia H.
Wu, Haodi
Paulk, Nicole K.
Liu, Lan Xiang
Kim, Namdoo
Kay, Mark A.
Wu, Joseph C.
Lin, Michael Z.
An orange calcium-modulated bioluminescent indicator for non-invasive activity imaging
title An orange calcium-modulated bioluminescent indicator for non-invasive activity imaging
title_full An orange calcium-modulated bioluminescent indicator for non-invasive activity imaging
title_fullStr An orange calcium-modulated bioluminescent indicator for non-invasive activity imaging
title_full_unstemmed An orange calcium-modulated bioluminescent indicator for non-invasive activity imaging
title_short An orange calcium-modulated bioluminescent indicator for non-invasive activity imaging
title_sort orange calcium-modulated bioluminescent indicator for non-invasive activity imaging
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6563924/
https://www.ncbi.nlm.nih.gov/pubmed/30936501
http://dx.doi.org/10.1038/s41589-019-0256-z
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