Field diagnosis and genotyping of chikungunya virus using a dried reverse transcription loop-mediated isothermal amplification (LAMP) assay and MinION sequencing

Detection and sequencing of chikungunya virus (CHIKV) genome was performed using a combination of a modified reverse transcription loop-mediated isothermal amplification (RT-LAMP) method and a MinION sequencer. We developed the protocol for drying all the reagents for the RT-LAMP in a single reactio...

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Detalles Bibliográficos
Autores principales: Hayashida, Kyoko, Orba, Yasuko, Sequeira, Patricia C., Sugimoto, Chihiro, Hall, William W., Eshita, Yuki, Suzuki, Yutaka, Runtuwene, Lucky, Brasil, Patricia, Calvet, Guilherme, Rodrigues, Cintia D. S., dos Santos, Carolina C., Mares-Guia, Maria A. M., Yamagishi, Junya, de Filippis, Ana M. B., Sawa, Hirofumi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6564047/
https://www.ncbi.nlm.nih.gov/pubmed/31158221
http://dx.doi.org/10.1371/journal.pntd.0007480

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