Cargando…

Plasmofluidic Microlenses for Label-Free Optical Sorting of Exosomes

Optical chromatography is a powerful optofluidic technique enabling label-free fractionation of microscopic bioparticles from heterogenous mixtures. However, sophisticated instrumentation requirements for precise alignment of optical scattering and fluidic drag forces is a fundamental shortcoming of...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhu, Xiangchao, Cicek, Ahmet, Li, Yixiang, Yanik, Ahmet Ali
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6565621/
https://www.ncbi.nlm.nih.gov/pubmed/31197196
http://dx.doi.org/10.1038/s41598-019-44801-3
_version_ 1783426683693432832
author Zhu, Xiangchao
Cicek, Ahmet
Li, Yixiang
Yanik, Ahmet Ali
author_facet Zhu, Xiangchao
Cicek, Ahmet
Li, Yixiang
Yanik, Ahmet Ali
author_sort Zhu, Xiangchao
collection PubMed
description Optical chromatography is a powerful optofluidic technique enabling label-free fractionation of microscopic bioparticles from heterogenous mixtures. However, sophisticated instrumentation requirements for precise alignment of optical scattering and fluidic drag forces is a fundamental shortcoming of this technique. Here, we introduce a subwavelength thick (<200 nm) Optofluidic PlasmonIC (OPtIC) microlens that effortlessly achieves objective-free focusing and self-alignment of opposing optical scattering and fluidic drag forces for selective separation of exosome size bioparticles. Our optofluidic microlens provides a self-collimating mechanism for particle trajectories with a spatial dispersion that is inherently minimized by the optical gradient and radial fluidic drag forces working together to align the particles along the optical axis. We demonstrate that this facile platform facilitates complete separation of small size bioparticles (i.e., exosomes) from a heterogenous mixture through negative depletion and provides a robust selective separation capability for same size nanoparticles based on their differences in chemical composition. Unlike existing optical chromatography techniques that require complicated instrumentation (lasers, objectives and precise alignment stages), our OPtIC microlenses with a foot-print of 4 μm × 4 μm open up the possibility of multiplexed and high-throughput sorting of nanoparticles on a chip using low-cost broadband light sources.
format Online
Article
Text
id pubmed-6565621
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-65656212019-06-20 Plasmofluidic Microlenses for Label-Free Optical Sorting of Exosomes Zhu, Xiangchao Cicek, Ahmet Li, Yixiang Yanik, Ahmet Ali Sci Rep Article Optical chromatography is a powerful optofluidic technique enabling label-free fractionation of microscopic bioparticles from heterogenous mixtures. However, sophisticated instrumentation requirements for precise alignment of optical scattering and fluidic drag forces is a fundamental shortcoming of this technique. Here, we introduce a subwavelength thick (<200 nm) Optofluidic PlasmonIC (OPtIC) microlens that effortlessly achieves objective-free focusing and self-alignment of opposing optical scattering and fluidic drag forces for selective separation of exosome size bioparticles. Our optofluidic microlens provides a self-collimating mechanism for particle trajectories with a spatial dispersion that is inherently minimized by the optical gradient and radial fluidic drag forces working together to align the particles along the optical axis. We demonstrate that this facile platform facilitates complete separation of small size bioparticles (i.e., exosomes) from a heterogenous mixture through negative depletion and provides a robust selective separation capability for same size nanoparticles based on their differences in chemical composition. Unlike existing optical chromatography techniques that require complicated instrumentation (lasers, objectives and precise alignment stages), our OPtIC microlenses with a foot-print of 4 μm × 4 μm open up the possibility of multiplexed and high-throughput sorting of nanoparticles on a chip using low-cost broadband light sources. Nature Publishing Group UK 2019-06-13 /pmc/articles/PMC6565621/ /pubmed/31197196 http://dx.doi.org/10.1038/s41598-019-44801-3 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Zhu, Xiangchao
Cicek, Ahmet
Li, Yixiang
Yanik, Ahmet Ali
Plasmofluidic Microlenses for Label-Free Optical Sorting of Exosomes
title Plasmofluidic Microlenses for Label-Free Optical Sorting of Exosomes
title_full Plasmofluidic Microlenses for Label-Free Optical Sorting of Exosomes
title_fullStr Plasmofluidic Microlenses for Label-Free Optical Sorting of Exosomes
title_full_unstemmed Plasmofluidic Microlenses for Label-Free Optical Sorting of Exosomes
title_short Plasmofluidic Microlenses for Label-Free Optical Sorting of Exosomes
title_sort plasmofluidic microlenses for label-free optical sorting of exosomes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6565621/
https://www.ncbi.nlm.nih.gov/pubmed/31197196
http://dx.doi.org/10.1038/s41598-019-44801-3
work_keys_str_mv AT zhuxiangchao plasmofluidicmicrolensesforlabelfreeopticalsortingofexosomes
AT cicekahmet plasmofluidicmicrolensesforlabelfreeopticalsortingofexosomes
AT liyixiang plasmofluidicmicrolensesforlabelfreeopticalsortingofexosomes
AT yanikahmetali plasmofluidicmicrolensesforlabelfreeopticalsortingofexosomes