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CD43 sialoglycoprotein modulates cardiac inflammation and murine susceptibility to Trypanosoma cruzi infection
CD43 (leukosialin) is a large sialoglycoprotein abundantly expressed on the surface of most cells from the hematopoietic lineage. CD43 is directly involved in the contact between cells participating in a series of events such as signaling, adherence and host parasite interactions. In this study we e...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6565700/ https://www.ncbi.nlm.nih.gov/pubmed/31197200 http://dx.doi.org/10.1038/s41598-019-45138-7 |
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author | Alisson-Silva, Frederico Mantuano, Natália Rodrigues Lopes, Ana Luiza Vasconcelos-dos-Santos, Andréia Vale, André Macedo Costa, Miriam Maria Cannon, Judy L. Oliveira, Ana Carolina Todeschini, Adriane R. |
author_facet | Alisson-Silva, Frederico Mantuano, Natália Rodrigues Lopes, Ana Luiza Vasconcelos-dos-Santos, Andréia Vale, André Macedo Costa, Miriam Maria Cannon, Judy L. Oliveira, Ana Carolina Todeschini, Adriane R. |
author_sort | Alisson-Silva, Frederico |
collection | PubMed |
description | CD43 (leukosialin) is a large sialoglycoprotein abundantly expressed on the surface of most cells from the hematopoietic lineage. CD43 is directly involved in the contact between cells participating in a series of events such as signaling, adherence and host parasite interactions. In this study we examined the role of CD43 in the immune response against Trypanosoma cruzi, the protozoan parasite that causes Chagas’ disease, a potential life-threatening illness endemic in 21 Latin American countries according to the WHO. The acute stage of infection is marked by intense parasitemia and cardiac tissue parasitism, resulting in the recruitment of inflammatory cells and acute damage to the heart tissue. We show here that CD43(−/−) mice were more resistant to infection due to increased cytotoxicity of antigen specific CD8+ T cells and reduced inflammatory infiltration in the cardiac tissue, both contributing to lower cardiomyocyte damage. In addition, we demonstrate that the induction of acute myocarditis involves the engagement of CD43 cytoplasmic tripeptide sequence KRR to ezrin-radixin-moiesin cytoskeletal proteins. Together, our results show the participation of CD43 in different events involved in the pathogenesis of T. cruzi infection, contributing to a better overall understanding of the mechanisms underlying the pathogenesis of acute chagasic cardiomyopathy. |
format | Online Article Text |
id | pubmed-6565700 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-65657002019-06-20 CD43 sialoglycoprotein modulates cardiac inflammation and murine susceptibility to Trypanosoma cruzi infection Alisson-Silva, Frederico Mantuano, Natália Rodrigues Lopes, Ana Luiza Vasconcelos-dos-Santos, Andréia Vale, André Macedo Costa, Miriam Maria Cannon, Judy L. Oliveira, Ana Carolina Todeschini, Adriane R. Sci Rep Article CD43 (leukosialin) is a large sialoglycoprotein abundantly expressed on the surface of most cells from the hematopoietic lineage. CD43 is directly involved in the contact between cells participating in a series of events such as signaling, adherence and host parasite interactions. In this study we examined the role of CD43 in the immune response against Trypanosoma cruzi, the protozoan parasite that causes Chagas’ disease, a potential life-threatening illness endemic in 21 Latin American countries according to the WHO. The acute stage of infection is marked by intense parasitemia and cardiac tissue parasitism, resulting in the recruitment of inflammatory cells and acute damage to the heart tissue. We show here that CD43(−/−) mice were more resistant to infection due to increased cytotoxicity of antigen specific CD8+ T cells and reduced inflammatory infiltration in the cardiac tissue, both contributing to lower cardiomyocyte damage. In addition, we demonstrate that the induction of acute myocarditis involves the engagement of CD43 cytoplasmic tripeptide sequence KRR to ezrin-radixin-moiesin cytoskeletal proteins. Together, our results show the participation of CD43 in different events involved in the pathogenesis of T. cruzi infection, contributing to a better overall understanding of the mechanisms underlying the pathogenesis of acute chagasic cardiomyopathy. Nature Publishing Group UK 2019-06-13 /pmc/articles/PMC6565700/ /pubmed/31197200 http://dx.doi.org/10.1038/s41598-019-45138-7 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Alisson-Silva, Frederico Mantuano, Natália Rodrigues Lopes, Ana Luiza Vasconcelos-dos-Santos, Andréia Vale, André Macedo Costa, Miriam Maria Cannon, Judy L. Oliveira, Ana Carolina Todeschini, Adriane R. CD43 sialoglycoprotein modulates cardiac inflammation and murine susceptibility to Trypanosoma cruzi infection |
title | CD43 sialoglycoprotein modulates cardiac inflammation and murine susceptibility to Trypanosoma cruzi infection |
title_full | CD43 sialoglycoprotein modulates cardiac inflammation and murine susceptibility to Trypanosoma cruzi infection |
title_fullStr | CD43 sialoglycoprotein modulates cardiac inflammation and murine susceptibility to Trypanosoma cruzi infection |
title_full_unstemmed | CD43 sialoglycoprotein modulates cardiac inflammation and murine susceptibility to Trypanosoma cruzi infection |
title_short | CD43 sialoglycoprotein modulates cardiac inflammation and murine susceptibility to Trypanosoma cruzi infection |
title_sort | cd43 sialoglycoprotein modulates cardiac inflammation and murine susceptibility to trypanosoma cruzi infection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6565700/ https://www.ncbi.nlm.nih.gov/pubmed/31197200 http://dx.doi.org/10.1038/s41598-019-45138-7 |
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