Cargando…

Abundance of bacterial Type VI secretion system components measured by targeted proteomics

The Type VI secretion system (T6SS) is important for bacterial competition as well as virulence in many Gram-negative bacteria and its dynamics and regulation varies significantly between species. To gain insights into the mechanisms regulating T6SS assembly, we apply targeted proteomics to determin...

Descripción completa

Detalles Bibliográficos
Autores principales: Lin, Lin, Lezan, Emmanuelle, Schmidt, Alexander, Basler, Marek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6565705/
https://www.ncbi.nlm.nih.gov/pubmed/31197144
http://dx.doi.org/10.1038/s41467-019-10466-9
_version_ 1783426703266152448
author Lin, Lin
Lezan, Emmanuelle
Schmidt, Alexander
Basler, Marek
author_facet Lin, Lin
Lezan, Emmanuelle
Schmidt, Alexander
Basler, Marek
author_sort Lin, Lin
collection PubMed
description The Type VI secretion system (T6SS) is important for bacterial competition as well as virulence in many Gram-negative bacteria and its dynamics and regulation varies significantly between species. To gain insights into the mechanisms regulating T6SS assembly, we apply targeted proteomics to determine the abundance of the key T6SS components in Vibrio cholerae, Pseudomonas aeruginosa and Acinetobacter baylyi. We show that while there are species specific exceptions, the abundance of most components is similar in all three bacteria and ranges from less than hundred to tens of thousands of copies per cell. The comparison of T6SS dynamics and protein abundance in V. cholerae grown under various conditions suggests that the critical component TssE and the secreted protein VasX are unstable and this diminishes T6SS assembly when protein synthesis is limited. Our quantitative analysis opens possibilities to build realistic models of T6SS assembly and to identify principles of T6SS regulation in various species.
format Online
Article
Text
id pubmed-6565705
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-65657052019-06-21 Abundance of bacterial Type VI secretion system components measured by targeted proteomics Lin, Lin Lezan, Emmanuelle Schmidt, Alexander Basler, Marek Nat Commun Article The Type VI secretion system (T6SS) is important for bacterial competition as well as virulence in many Gram-negative bacteria and its dynamics and regulation varies significantly between species. To gain insights into the mechanisms regulating T6SS assembly, we apply targeted proteomics to determine the abundance of the key T6SS components in Vibrio cholerae, Pseudomonas aeruginosa and Acinetobacter baylyi. We show that while there are species specific exceptions, the abundance of most components is similar in all three bacteria and ranges from less than hundred to tens of thousands of copies per cell. The comparison of T6SS dynamics and protein abundance in V. cholerae grown under various conditions suggests that the critical component TssE and the secreted protein VasX are unstable and this diminishes T6SS assembly when protein synthesis is limited. Our quantitative analysis opens possibilities to build realistic models of T6SS assembly and to identify principles of T6SS regulation in various species. Nature Publishing Group UK 2019-06-13 /pmc/articles/PMC6565705/ /pubmed/31197144 http://dx.doi.org/10.1038/s41467-019-10466-9 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Lin, Lin
Lezan, Emmanuelle
Schmidt, Alexander
Basler, Marek
Abundance of bacterial Type VI secretion system components measured by targeted proteomics
title Abundance of bacterial Type VI secretion system components measured by targeted proteomics
title_full Abundance of bacterial Type VI secretion system components measured by targeted proteomics
title_fullStr Abundance of bacterial Type VI secretion system components measured by targeted proteomics
title_full_unstemmed Abundance of bacterial Type VI secretion system components measured by targeted proteomics
title_short Abundance of bacterial Type VI secretion system components measured by targeted proteomics
title_sort abundance of bacterial type vi secretion system components measured by targeted proteomics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6565705/
https://www.ncbi.nlm.nih.gov/pubmed/31197144
http://dx.doi.org/10.1038/s41467-019-10466-9
work_keys_str_mv AT linlin abundanceofbacterialtypevisecretionsystemcomponentsmeasuredbytargetedproteomics
AT lezanemmanuelle abundanceofbacterialtypevisecretionsystemcomponentsmeasuredbytargetedproteomics
AT schmidtalexander abundanceofbacterialtypevisecretionsystemcomponentsmeasuredbytargetedproteomics
AT baslermarek abundanceofbacterialtypevisecretionsystemcomponentsmeasuredbytargetedproteomics