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Abundance of bacterial Type VI secretion system components measured by targeted proteomics
The Type VI secretion system (T6SS) is important for bacterial competition as well as virulence in many Gram-negative bacteria and its dynamics and regulation varies significantly between species. To gain insights into the mechanisms regulating T6SS assembly, we apply targeted proteomics to determin...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6565705/ https://www.ncbi.nlm.nih.gov/pubmed/31197144 http://dx.doi.org/10.1038/s41467-019-10466-9 |
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author | Lin, Lin Lezan, Emmanuelle Schmidt, Alexander Basler, Marek |
author_facet | Lin, Lin Lezan, Emmanuelle Schmidt, Alexander Basler, Marek |
author_sort | Lin, Lin |
collection | PubMed |
description | The Type VI secretion system (T6SS) is important for bacterial competition as well as virulence in many Gram-negative bacteria and its dynamics and regulation varies significantly between species. To gain insights into the mechanisms regulating T6SS assembly, we apply targeted proteomics to determine the abundance of the key T6SS components in Vibrio cholerae, Pseudomonas aeruginosa and Acinetobacter baylyi. We show that while there are species specific exceptions, the abundance of most components is similar in all three bacteria and ranges from less than hundred to tens of thousands of copies per cell. The comparison of T6SS dynamics and protein abundance in V. cholerae grown under various conditions suggests that the critical component TssE and the secreted protein VasX are unstable and this diminishes T6SS assembly when protein synthesis is limited. Our quantitative analysis opens possibilities to build realistic models of T6SS assembly and to identify principles of T6SS regulation in various species. |
format | Online Article Text |
id | pubmed-6565705 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-65657052019-06-21 Abundance of bacterial Type VI secretion system components measured by targeted proteomics Lin, Lin Lezan, Emmanuelle Schmidt, Alexander Basler, Marek Nat Commun Article The Type VI secretion system (T6SS) is important for bacterial competition as well as virulence in many Gram-negative bacteria and its dynamics and regulation varies significantly between species. To gain insights into the mechanisms regulating T6SS assembly, we apply targeted proteomics to determine the abundance of the key T6SS components in Vibrio cholerae, Pseudomonas aeruginosa and Acinetobacter baylyi. We show that while there are species specific exceptions, the abundance of most components is similar in all three bacteria and ranges from less than hundred to tens of thousands of copies per cell. The comparison of T6SS dynamics and protein abundance in V. cholerae grown under various conditions suggests that the critical component TssE and the secreted protein VasX are unstable and this diminishes T6SS assembly when protein synthesis is limited. Our quantitative analysis opens possibilities to build realistic models of T6SS assembly and to identify principles of T6SS regulation in various species. Nature Publishing Group UK 2019-06-13 /pmc/articles/PMC6565705/ /pubmed/31197144 http://dx.doi.org/10.1038/s41467-019-10466-9 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Lin, Lin Lezan, Emmanuelle Schmidt, Alexander Basler, Marek Abundance of bacterial Type VI secretion system components measured by targeted proteomics |
title | Abundance of bacterial Type VI secretion system components measured by targeted proteomics |
title_full | Abundance of bacterial Type VI secretion system components measured by targeted proteomics |
title_fullStr | Abundance of bacterial Type VI secretion system components measured by targeted proteomics |
title_full_unstemmed | Abundance of bacterial Type VI secretion system components measured by targeted proteomics |
title_short | Abundance of bacterial Type VI secretion system components measured by targeted proteomics |
title_sort | abundance of bacterial type vi secretion system components measured by targeted proteomics |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6565705/ https://www.ncbi.nlm.nih.gov/pubmed/31197144 http://dx.doi.org/10.1038/s41467-019-10466-9 |
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