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A2A-D2 Heteromers on Striatal Astrocytes: Biochemical and Biophysical Evidence
Our previous findings indicate that A2A and D2 receptors are co-expressed on adult rat striatal astrocytes and on the astrocyte processes, and that A2A-D2 receptor–receptor interaction can control the release of glutamate from the processes. Functional evidence suggests that the receptor–receptor in...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6566402/ https://www.ncbi.nlm.nih.gov/pubmed/31109007 http://dx.doi.org/10.3390/ijms20102457 |
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author | Pelassa, Simone Guidolin, Diego Venturini, Arianna Averna, Monica Frumento, Giulia Campanini, Letizia Bernardi, Rosa Cortelli, Pietro Calandra Buonaura, Giovanna Maura, Guido Agnati, Luigi F. Cervetto, Chiara Marcoli, Manuela |
author_facet | Pelassa, Simone Guidolin, Diego Venturini, Arianna Averna, Monica Frumento, Giulia Campanini, Letizia Bernardi, Rosa Cortelli, Pietro Calandra Buonaura, Giovanna Maura, Guido Agnati, Luigi F. Cervetto, Chiara Marcoli, Manuela |
author_sort | Pelassa, Simone |
collection | PubMed |
description | Our previous findings indicate that A2A and D2 receptors are co-expressed on adult rat striatal astrocytes and on the astrocyte processes, and that A2A-D2 receptor–receptor interaction can control the release of glutamate from the processes. Functional evidence suggests that the receptor–receptor interaction was based on heteromerization of native A2A and D2 receptors at the plasma membrane of striatal astrocyte processes. We here provide biochemical and biophysical evidence confirming that receptor–receptor interaction between A2A and D2 receptors at the astrocyte plasma membrane is based on A2A-D2 heteromerization. To our knowledge, this is the first direct demonstration of the ability of native A2A and D2 receptors to heteromerize on glial cells. As striatal astrocytes are recognized to be involved in Parkinson’s pathophysiology, the findings that adenosine A2A and dopamine D2 receptors can form A2A-D2 heteromers on the astrocytes in the striatum (and that these heteromers can play roles in the control of the striatal glutamatergic transmission) may shed light on the molecular mechanisms involved in the pathogenesis of the disease. |
format | Online Article Text |
id | pubmed-6566402 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-65664022019-06-17 A2A-D2 Heteromers on Striatal Astrocytes: Biochemical and Biophysical Evidence Pelassa, Simone Guidolin, Diego Venturini, Arianna Averna, Monica Frumento, Giulia Campanini, Letizia Bernardi, Rosa Cortelli, Pietro Calandra Buonaura, Giovanna Maura, Guido Agnati, Luigi F. Cervetto, Chiara Marcoli, Manuela Int J Mol Sci Communication Our previous findings indicate that A2A and D2 receptors are co-expressed on adult rat striatal astrocytes and on the astrocyte processes, and that A2A-D2 receptor–receptor interaction can control the release of glutamate from the processes. Functional evidence suggests that the receptor–receptor interaction was based on heteromerization of native A2A and D2 receptors at the plasma membrane of striatal astrocyte processes. We here provide biochemical and biophysical evidence confirming that receptor–receptor interaction between A2A and D2 receptors at the astrocyte plasma membrane is based on A2A-D2 heteromerization. To our knowledge, this is the first direct demonstration of the ability of native A2A and D2 receptors to heteromerize on glial cells. As striatal astrocytes are recognized to be involved in Parkinson’s pathophysiology, the findings that adenosine A2A and dopamine D2 receptors can form A2A-D2 heteromers on the astrocytes in the striatum (and that these heteromers can play roles in the control of the striatal glutamatergic transmission) may shed light on the molecular mechanisms involved in the pathogenesis of the disease. MDPI 2019-05-17 /pmc/articles/PMC6566402/ /pubmed/31109007 http://dx.doi.org/10.3390/ijms20102457 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Pelassa, Simone Guidolin, Diego Venturini, Arianna Averna, Monica Frumento, Giulia Campanini, Letizia Bernardi, Rosa Cortelli, Pietro Calandra Buonaura, Giovanna Maura, Guido Agnati, Luigi F. Cervetto, Chiara Marcoli, Manuela A2A-D2 Heteromers on Striatal Astrocytes: Biochemical and Biophysical Evidence |
title | A2A-D2 Heteromers on Striatal Astrocytes: Biochemical and Biophysical Evidence |
title_full | A2A-D2 Heteromers on Striatal Astrocytes: Biochemical and Biophysical Evidence |
title_fullStr | A2A-D2 Heteromers on Striatal Astrocytes: Biochemical and Biophysical Evidence |
title_full_unstemmed | A2A-D2 Heteromers on Striatal Astrocytes: Biochemical and Biophysical Evidence |
title_short | A2A-D2 Heteromers on Striatal Astrocytes: Biochemical and Biophysical Evidence |
title_sort | a2a-d2 heteromers on striatal astrocytes: biochemical and biophysical evidence |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6566402/ https://www.ncbi.nlm.nih.gov/pubmed/31109007 http://dx.doi.org/10.3390/ijms20102457 |
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