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A2A-D2 Heteromers on Striatal Astrocytes: Biochemical and Biophysical Evidence

Our previous findings indicate that A2A and D2 receptors are co-expressed on adult rat striatal astrocytes and on the astrocyte processes, and that A2A-D2 receptor–receptor interaction can control the release of glutamate from the processes. Functional evidence suggests that the receptor–receptor in...

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Autores principales: Pelassa, Simone, Guidolin, Diego, Venturini, Arianna, Averna, Monica, Frumento, Giulia, Campanini, Letizia, Bernardi, Rosa, Cortelli, Pietro, Calandra Buonaura, Giovanna, Maura, Guido, Agnati, Luigi F., Cervetto, Chiara, Marcoli, Manuela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6566402/
https://www.ncbi.nlm.nih.gov/pubmed/31109007
http://dx.doi.org/10.3390/ijms20102457
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author Pelassa, Simone
Guidolin, Diego
Venturini, Arianna
Averna, Monica
Frumento, Giulia
Campanini, Letizia
Bernardi, Rosa
Cortelli, Pietro
Calandra Buonaura, Giovanna
Maura, Guido
Agnati, Luigi F.
Cervetto, Chiara
Marcoli, Manuela
author_facet Pelassa, Simone
Guidolin, Diego
Venturini, Arianna
Averna, Monica
Frumento, Giulia
Campanini, Letizia
Bernardi, Rosa
Cortelli, Pietro
Calandra Buonaura, Giovanna
Maura, Guido
Agnati, Luigi F.
Cervetto, Chiara
Marcoli, Manuela
author_sort Pelassa, Simone
collection PubMed
description Our previous findings indicate that A2A and D2 receptors are co-expressed on adult rat striatal astrocytes and on the astrocyte processes, and that A2A-D2 receptor–receptor interaction can control the release of glutamate from the processes. Functional evidence suggests that the receptor–receptor interaction was based on heteromerization of native A2A and D2 receptors at the plasma membrane of striatal astrocyte processes. We here provide biochemical and biophysical evidence confirming that receptor–receptor interaction between A2A and D2 receptors at the astrocyte plasma membrane is based on A2A-D2 heteromerization. To our knowledge, this is the first direct demonstration of the ability of native A2A and D2 receptors to heteromerize on glial cells. As striatal astrocytes are recognized to be involved in Parkinson’s pathophysiology, the findings that adenosine A2A and dopamine D2 receptors can form A2A-D2 heteromers on the astrocytes in the striatum (and that these heteromers can play roles in the control of the striatal glutamatergic transmission) may shed light on the molecular mechanisms involved in the pathogenesis of the disease.
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spelling pubmed-65664022019-06-17 A2A-D2 Heteromers on Striatal Astrocytes: Biochemical and Biophysical Evidence Pelassa, Simone Guidolin, Diego Venturini, Arianna Averna, Monica Frumento, Giulia Campanini, Letizia Bernardi, Rosa Cortelli, Pietro Calandra Buonaura, Giovanna Maura, Guido Agnati, Luigi F. Cervetto, Chiara Marcoli, Manuela Int J Mol Sci Communication Our previous findings indicate that A2A and D2 receptors are co-expressed on adult rat striatal astrocytes and on the astrocyte processes, and that A2A-D2 receptor–receptor interaction can control the release of glutamate from the processes. Functional evidence suggests that the receptor–receptor interaction was based on heteromerization of native A2A and D2 receptors at the plasma membrane of striatal astrocyte processes. We here provide biochemical and biophysical evidence confirming that receptor–receptor interaction between A2A and D2 receptors at the astrocyte plasma membrane is based on A2A-D2 heteromerization. To our knowledge, this is the first direct demonstration of the ability of native A2A and D2 receptors to heteromerize on glial cells. As striatal astrocytes are recognized to be involved in Parkinson’s pathophysiology, the findings that adenosine A2A and dopamine D2 receptors can form A2A-D2 heteromers on the astrocytes in the striatum (and that these heteromers can play roles in the control of the striatal glutamatergic transmission) may shed light on the molecular mechanisms involved in the pathogenesis of the disease. MDPI 2019-05-17 /pmc/articles/PMC6566402/ /pubmed/31109007 http://dx.doi.org/10.3390/ijms20102457 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Pelassa, Simone
Guidolin, Diego
Venturini, Arianna
Averna, Monica
Frumento, Giulia
Campanini, Letizia
Bernardi, Rosa
Cortelli, Pietro
Calandra Buonaura, Giovanna
Maura, Guido
Agnati, Luigi F.
Cervetto, Chiara
Marcoli, Manuela
A2A-D2 Heteromers on Striatal Astrocytes: Biochemical and Biophysical Evidence
title A2A-D2 Heteromers on Striatal Astrocytes: Biochemical and Biophysical Evidence
title_full A2A-D2 Heteromers on Striatal Astrocytes: Biochemical and Biophysical Evidence
title_fullStr A2A-D2 Heteromers on Striatal Astrocytes: Biochemical and Biophysical Evidence
title_full_unstemmed A2A-D2 Heteromers on Striatal Astrocytes: Biochemical and Biophysical Evidence
title_short A2A-D2 Heteromers on Striatal Astrocytes: Biochemical and Biophysical Evidence
title_sort a2a-d2 heteromers on striatal astrocytes: biochemical and biophysical evidence
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6566402/
https://www.ncbi.nlm.nih.gov/pubmed/31109007
http://dx.doi.org/10.3390/ijms20102457
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