Cargando…

Facile and Controllable Fabrication of Protein-Only Nanoparticles through Photo-Induced Crosslinking of Albumin and Their Application as DOX Carriers

Protein-based nanoparticles, as an alternative to conventional polymer-based nanoparticles, offer great advantages in biomedical applications owing to their functional and biocompatible characteristics. However, the route of fabrication towards protein-based nanoparticles faces substantial challenge...

Descripción completa

Detalles Bibliográficos
Autores principales: Long, Xiangyu, Ren, Jun, Zhang, Chao, Ji, Fangling, Jia, Lingyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6566423/
https://www.ncbi.nlm.nih.gov/pubmed/31137647
http://dx.doi.org/10.3390/nano9050797
_version_ 1783426849171308544
author Long, Xiangyu
Ren, Jun
Zhang, Chao
Ji, Fangling
Jia, Lingyun
author_facet Long, Xiangyu
Ren, Jun
Zhang, Chao
Ji, Fangling
Jia, Lingyun
author_sort Long, Xiangyu
collection PubMed
description Protein-based nanoparticles, as an alternative to conventional polymer-based nanoparticles, offer great advantages in biomedical applications owing to their functional and biocompatible characteristics. However, the route of fabrication towards protein-based nanoparticles faces substantial challenges, including limitations in size control and unavoidable usage of toxic crosslinkers or organic solvents, which may raise safety concerns related to products and their degradation components. In the present study, a photo-induced crosslinking approach was developed to prepare stable, size-controlled protein-only nanoparticles. The facile one-step reaction irradiated by visible light enables the formation of monodispersed bovine serum albumin nanoparticles (BSA NPs) within several minutes through a tyrosine photo-redox reaction, requiring no cross-linking agents. The size of the BSA NPs could be precisely manipulated (from 20 to 100 nm) by controlling the duration time of illumination. The resultant BSA NPs exhibited spherical morphology, and the α-helix structure in BSA was preserved. Further study demonstrated that the 35 nm doxorubicin (DOX)-loaded BSA NPs achieved a drug loading content of 6.3%, encapsulation efficiency of 70.7%, and a controlled release profile with responsivity to both pH and reducing conditions. Importantly, the in vitro drug delivery experiment demonstrated efficient cellular internalizations of the DOX-loaded BSA NPs and inhibitory activities on MCF-7 and HeLa cells. This method shows the promise of being a platform for the green synthesis of protein-only nanoparticles for biomedical applications.
format Online
Article
Text
id pubmed-6566423
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-65664232019-06-17 Facile and Controllable Fabrication of Protein-Only Nanoparticles through Photo-Induced Crosslinking of Albumin and Their Application as DOX Carriers Long, Xiangyu Ren, Jun Zhang, Chao Ji, Fangling Jia, Lingyun Nanomaterials (Basel) Article Protein-based nanoparticles, as an alternative to conventional polymer-based nanoparticles, offer great advantages in biomedical applications owing to their functional and biocompatible characteristics. However, the route of fabrication towards protein-based nanoparticles faces substantial challenges, including limitations in size control and unavoidable usage of toxic crosslinkers or organic solvents, which may raise safety concerns related to products and their degradation components. In the present study, a photo-induced crosslinking approach was developed to prepare stable, size-controlled protein-only nanoparticles. The facile one-step reaction irradiated by visible light enables the formation of monodispersed bovine serum albumin nanoparticles (BSA NPs) within several minutes through a tyrosine photo-redox reaction, requiring no cross-linking agents. The size of the BSA NPs could be precisely manipulated (from 20 to 100 nm) by controlling the duration time of illumination. The resultant BSA NPs exhibited spherical morphology, and the α-helix structure in BSA was preserved. Further study demonstrated that the 35 nm doxorubicin (DOX)-loaded BSA NPs achieved a drug loading content of 6.3%, encapsulation efficiency of 70.7%, and a controlled release profile with responsivity to both pH and reducing conditions. Importantly, the in vitro drug delivery experiment demonstrated efficient cellular internalizations of the DOX-loaded BSA NPs and inhibitory activities on MCF-7 and HeLa cells. This method shows the promise of being a platform for the green synthesis of protein-only nanoparticles for biomedical applications. MDPI 2019-05-24 /pmc/articles/PMC6566423/ /pubmed/31137647 http://dx.doi.org/10.3390/nano9050797 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Long, Xiangyu
Ren, Jun
Zhang, Chao
Ji, Fangling
Jia, Lingyun
Facile and Controllable Fabrication of Protein-Only Nanoparticles through Photo-Induced Crosslinking of Albumin and Their Application as DOX Carriers
title Facile and Controllable Fabrication of Protein-Only Nanoparticles through Photo-Induced Crosslinking of Albumin and Their Application as DOX Carriers
title_full Facile and Controllable Fabrication of Protein-Only Nanoparticles through Photo-Induced Crosslinking of Albumin and Their Application as DOX Carriers
title_fullStr Facile and Controllable Fabrication of Protein-Only Nanoparticles through Photo-Induced Crosslinking of Albumin and Their Application as DOX Carriers
title_full_unstemmed Facile and Controllable Fabrication of Protein-Only Nanoparticles through Photo-Induced Crosslinking of Albumin and Their Application as DOX Carriers
title_short Facile and Controllable Fabrication of Protein-Only Nanoparticles through Photo-Induced Crosslinking of Albumin and Their Application as DOX Carriers
title_sort facile and controllable fabrication of protein-only nanoparticles through photo-induced crosslinking of albumin and their application as dox carriers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6566423/
https://www.ncbi.nlm.nih.gov/pubmed/31137647
http://dx.doi.org/10.3390/nano9050797
work_keys_str_mv AT longxiangyu facileandcontrollablefabricationofproteinonlynanoparticlesthroughphotoinducedcrosslinkingofalbuminandtheirapplicationasdoxcarriers
AT renjun facileandcontrollablefabricationofproteinonlynanoparticlesthroughphotoinducedcrosslinkingofalbuminandtheirapplicationasdoxcarriers
AT zhangchao facileandcontrollablefabricationofproteinonlynanoparticlesthroughphotoinducedcrosslinkingofalbuminandtheirapplicationasdoxcarriers
AT jifangling facileandcontrollablefabricationofproteinonlynanoparticlesthroughphotoinducedcrosslinkingofalbuminandtheirapplicationasdoxcarriers
AT jialingyun facileandcontrollablefabricationofproteinonlynanoparticlesthroughphotoinducedcrosslinkingofalbuminandtheirapplicationasdoxcarriers