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Structural Influence on the Post-Clustering Stability of DNA/AgNCs Fluorescence

DNA-encapsulated Silver Nanoclusters (DNA/AgNCs) based sensors have gained increasing attention in past years due to their diverse applications in bioimaging, biosensing, and enzymatic assays. Given the potential of DNA/AgNCs for practical applications, the systematic studies of the fluorescent stab...

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Autores principales: Nagda, Riddhi, Shah, Pratik, Lee, Chang Seop, Park, Sooyeon, Yang, Seong Wook
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6566520/
https://www.ncbi.nlm.nih.gov/pubmed/31035341
http://dx.doi.org/10.3390/nano9050667
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author Nagda, Riddhi
Shah, Pratik
Lee, Chang Seop
Park, Sooyeon
Yang, Seong Wook
author_facet Nagda, Riddhi
Shah, Pratik
Lee, Chang Seop
Park, Sooyeon
Yang, Seong Wook
author_sort Nagda, Riddhi
collection PubMed
description DNA-encapsulated Silver Nanoclusters (DNA/AgNCs) based sensors have gained increasing attention in past years due to their diverse applications in bioimaging, biosensing, and enzymatic assays. Given the potential of DNA/AgNCs for practical applications, the systematic studies of the fluorescent stability over an extended period is necessary. However, the correlation between nucleic acid properties and the long-term stability of DNA/AgNCs is less known. With locking-to-unlocking sensors, in which the secondary structure of DNA template is standardized, we investigated the correlation between the DNA structure and the fluorescence stability of AgNCs. Post-synthesis of DNA/AgNCs, the fluorescence, and structures of templates were monitored over three weeks. By combining the fluorescence spectroscopy with the in-gel fluorescent assay, we found that AgNCs encapsulated by dimer-structured DNA/AgNCs templates were more stable than those of hairpin-structured DNA/AgNCs templates. While the orange fluorescence from the dimer templates increased over three weeks, the red fluorescence from the hairpin templates was diminished by >80% within two days at room temperature. Further tests revealed that hairpin-encapsulated red-emissive AgNCs is more sensitive to oxidation by atmospheric oxygen compared to dimer encapsulated orange AgNCs. Our observations may provide an important clue in encapsulating photophysically more stable AgNCs by tuning the DNA secondary structures. The proposed strategy here can be essential for pragmatic applications of DNA/AgNCs templates.
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spelling pubmed-65665202019-06-17 Structural Influence on the Post-Clustering Stability of DNA/AgNCs Fluorescence Nagda, Riddhi Shah, Pratik Lee, Chang Seop Park, Sooyeon Yang, Seong Wook Nanomaterials (Basel) Article DNA-encapsulated Silver Nanoclusters (DNA/AgNCs) based sensors have gained increasing attention in past years due to their diverse applications in bioimaging, biosensing, and enzymatic assays. Given the potential of DNA/AgNCs for practical applications, the systematic studies of the fluorescent stability over an extended period is necessary. However, the correlation between nucleic acid properties and the long-term stability of DNA/AgNCs is less known. With locking-to-unlocking sensors, in which the secondary structure of DNA template is standardized, we investigated the correlation between the DNA structure and the fluorescence stability of AgNCs. Post-synthesis of DNA/AgNCs, the fluorescence, and structures of templates were monitored over three weeks. By combining the fluorescence spectroscopy with the in-gel fluorescent assay, we found that AgNCs encapsulated by dimer-structured DNA/AgNCs templates were more stable than those of hairpin-structured DNA/AgNCs templates. While the orange fluorescence from the dimer templates increased over three weeks, the red fluorescence from the hairpin templates was diminished by >80% within two days at room temperature. Further tests revealed that hairpin-encapsulated red-emissive AgNCs is more sensitive to oxidation by atmospheric oxygen compared to dimer encapsulated orange AgNCs. Our observations may provide an important clue in encapsulating photophysically more stable AgNCs by tuning the DNA secondary structures. The proposed strategy here can be essential for pragmatic applications of DNA/AgNCs templates. MDPI 2019-04-28 /pmc/articles/PMC6566520/ /pubmed/31035341 http://dx.doi.org/10.3390/nano9050667 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nagda, Riddhi
Shah, Pratik
Lee, Chang Seop
Park, Sooyeon
Yang, Seong Wook
Structural Influence on the Post-Clustering Stability of DNA/AgNCs Fluorescence
title Structural Influence on the Post-Clustering Stability of DNA/AgNCs Fluorescence
title_full Structural Influence on the Post-Clustering Stability of DNA/AgNCs Fluorescence
title_fullStr Structural Influence on the Post-Clustering Stability of DNA/AgNCs Fluorescence
title_full_unstemmed Structural Influence on the Post-Clustering Stability of DNA/AgNCs Fluorescence
title_short Structural Influence on the Post-Clustering Stability of DNA/AgNCs Fluorescence
title_sort structural influence on the post-clustering stability of dna/agncs fluorescence
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6566520/
https://www.ncbi.nlm.nih.gov/pubmed/31035341
http://dx.doi.org/10.3390/nano9050667
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