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The Effect of Yearly-Dose Vitamin D Supplementation on Muscle Function in Mice

Supplementation with vitamin D helps to alleviate weakness and fatigue seen with deficiency. However, large bolus doses appear to worsen the risk of falls. Whether this occurs as a direct result of muscle weakness is currently unknown. Thus, the aims of this study were to examine the muscle function...

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Autores principales: Hayes, Alan, Rybalka, Emma, Debruin, Danielle A., Hanson, Erik D., Scott, David, Sanders, Kerrie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6566598/
https://www.ncbi.nlm.nih.gov/pubmed/31108908
http://dx.doi.org/10.3390/nu11051097
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author Hayes, Alan
Rybalka, Emma
Debruin, Danielle A.
Hanson, Erik D.
Scott, David
Sanders, Kerrie
author_facet Hayes, Alan
Rybalka, Emma
Debruin, Danielle A.
Hanson, Erik D.
Scott, David
Sanders, Kerrie
author_sort Hayes, Alan
collection PubMed
description Supplementation with vitamin D helps to alleviate weakness and fatigue seen with deficiency. However, large bolus doses appear to worsen the risk of falls. Whether this occurs as a direct result of muscle weakness is currently unknown. Thus, the aims of this study were to examine the muscle function following administration of high doses of vitamin D. Given the safety issues associated with bolus doses, experiments were conducted on C57BL6 mice. Mice at eight weeks of age with otherwise normal levels of vitamin D were supplemented for four weeks with a high dose (HIGH; n = 12) of vitamin D (20000 IU/kg food) designed to provide a year’s worth of vitamin D. These mice were compared to another group who received that same yearly dose in a single bolus i.p. injection (YEAR; n = 12). Mice provided with standard mouse chow, which contained 1000 IU/kg food, and injected with the vitamin D vehicle were used as controls (CON; n = 16). Force and fatigue properties of hind limb fast- and slow-twitch muscles were measured. CON animals ingested vitamin D consistent with typical human supplementation. HIGH animals consumed significantly more food than the CON animals, such that they ingested more than a year’s worth of vitamin D in four weeks. Despite this, there were few differences in the muscle function compared with CON. YEAR animals demonstrated lower absolute and relative forces in both muscles compared to the HIGH animals, as well as lower force during fatigue and early recovery. Large bolus doses of vitamin D appear to have detrimental effects on the skeletal muscle function, likely being a contributor to increased risk of falls observed with similar doses in humans. Mice ingesting the same amount over four weeks did not demonstrate the same deleterious effects, suggesting this may be a safe way to provide high vitamin D if required.
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spelling pubmed-65665982019-06-17 The Effect of Yearly-Dose Vitamin D Supplementation on Muscle Function in Mice Hayes, Alan Rybalka, Emma Debruin, Danielle A. Hanson, Erik D. Scott, David Sanders, Kerrie Nutrients Article Supplementation with vitamin D helps to alleviate weakness and fatigue seen with deficiency. However, large bolus doses appear to worsen the risk of falls. Whether this occurs as a direct result of muscle weakness is currently unknown. Thus, the aims of this study were to examine the muscle function following administration of high doses of vitamin D. Given the safety issues associated with bolus doses, experiments were conducted on C57BL6 mice. Mice at eight weeks of age with otherwise normal levels of vitamin D were supplemented for four weeks with a high dose (HIGH; n = 12) of vitamin D (20000 IU/kg food) designed to provide a year’s worth of vitamin D. These mice were compared to another group who received that same yearly dose in a single bolus i.p. injection (YEAR; n = 12). Mice provided with standard mouse chow, which contained 1000 IU/kg food, and injected with the vitamin D vehicle were used as controls (CON; n = 16). Force and fatigue properties of hind limb fast- and slow-twitch muscles were measured. CON animals ingested vitamin D consistent with typical human supplementation. HIGH animals consumed significantly more food than the CON animals, such that they ingested more than a year’s worth of vitamin D in four weeks. Despite this, there were few differences in the muscle function compared with CON. YEAR animals demonstrated lower absolute and relative forces in both muscles compared to the HIGH animals, as well as lower force during fatigue and early recovery. Large bolus doses of vitamin D appear to have detrimental effects on the skeletal muscle function, likely being a contributor to increased risk of falls observed with similar doses in humans. Mice ingesting the same amount over four weeks did not demonstrate the same deleterious effects, suggesting this may be a safe way to provide high vitamin D if required. MDPI 2019-05-17 /pmc/articles/PMC6566598/ /pubmed/31108908 http://dx.doi.org/10.3390/nu11051097 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hayes, Alan
Rybalka, Emma
Debruin, Danielle A.
Hanson, Erik D.
Scott, David
Sanders, Kerrie
The Effect of Yearly-Dose Vitamin D Supplementation on Muscle Function in Mice
title The Effect of Yearly-Dose Vitamin D Supplementation on Muscle Function in Mice
title_full The Effect of Yearly-Dose Vitamin D Supplementation on Muscle Function in Mice
title_fullStr The Effect of Yearly-Dose Vitamin D Supplementation on Muscle Function in Mice
title_full_unstemmed The Effect of Yearly-Dose Vitamin D Supplementation on Muscle Function in Mice
title_short The Effect of Yearly-Dose Vitamin D Supplementation on Muscle Function in Mice
title_sort effect of yearly-dose vitamin d supplementation on muscle function in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6566598/
https://www.ncbi.nlm.nih.gov/pubmed/31108908
http://dx.doi.org/10.3390/nu11051097
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