Cargando…
Dietary Egg Sphingomyelin Prevents Aortic Root Plaque Accumulation in Apolipoprotein-E Knockout Mice
Western-style diets have been linked with dyslipidemia and inflammation, two well-known risk factors associated with cardiovascular disease (CVD). Dietary sphingomyelin (SM) has been reported to modulate gut microbiota, and lower serum lipids and inflammation in mice on Western-style diets. However,...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6566691/ https://www.ncbi.nlm.nih.gov/pubmed/31117179 http://dx.doi.org/10.3390/nu11051124 |
_version_ | 1783426906392100864 |
---|---|
author | Millar, Courtney L. Norris, Gregory H. Vitols, Addison Garcia, Chelsea Seibel, Samantha Anto, Liya Blesso, Christopher N. |
author_facet | Millar, Courtney L. Norris, Gregory H. Vitols, Addison Garcia, Chelsea Seibel, Samantha Anto, Liya Blesso, Christopher N. |
author_sort | Millar, Courtney L. |
collection | PubMed |
description | Western-style diets have been linked with dyslipidemia and inflammation, two well-known risk factors associated with cardiovascular disease (CVD). Dietary sphingomyelin (SM) has been reported to modulate gut microbiota, and lower serum lipids and inflammation in mice on Western-style diets. However, few studies have examined if nutritionally-relevant intake of dietary SM can impact atherosclerosis progression. Thus, the aim of this study was to determine if incorporating 0.1% (w/w) egg SM (ESM) (equivalent to ~750 mg/day in humans) into a high-fat (45% kcal), cholesterol-enriched diet (HFD) could prevent atheroprogression in apoE(−/−) mice (n = 15/group). We found that mice fed with the ESM-rich diet had significantly lower epididymal fat mass (−46%) and tended to have higher spleen weights (+15%). There were no significant differences in serum lipids between groups. However, ESM-fed mice had significantly lower alanine aminotransferase (ALT) activity. Additionally, ESM-fed mice displayed significantly less aortic root lipid accumulation (−31%) compared to controls. This improvement in atherosclerosis was paired with over a two-fold reduction in circulating serum amyloid A (SAA) in ESM-fed mice. Finally, there was also a modulation of the gut microbiota with ESM supplementation. ESM may have the potential to prevent atherosclerosis, however further research in the clinical setting is warranted. |
format | Online Article Text |
id | pubmed-6566691 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-65666912019-06-17 Dietary Egg Sphingomyelin Prevents Aortic Root Plaque Accumulation in Apolipoprotein-E Knockout Mice Millar, Courtney L. Norris, Gregory H. Vitols, Addison Garcia, Chelsea Seibel, Samantha Anto, Liya Blesso, Christopher N. Nutrients Article Western-style diets have been linked with dyslipidemia and inflammation, two well-known risk factors associated with cardiovascular disease (CVD). Dietary sphingomyelin (SM) has been reported to modulate gut microbiota, and lower serum lipids and inflammation in mice on Western-style diets. However, few studies have examined if nutritionally-relevant intake of dietary SM can impact atherosclerosis progression. Thus, the aim of this study was to determine if incorporating 0.1% (w/w) egg SM (ESM) (equivalent to ~750 mg/day in humans) into a high-fat (45% kcal), cholesterol-enriched diet (HFD) could prevent atheroprogression in apoE(−/−) mice (n = 15/group). We found that mice fed with the ESM-rich diet had significantly lower epididymal fat mass (−46%) and tended to have higher spleen weights (+15%). There were no significant differences in serum lipids between groups. However, ESM-fed mice had significantly lower alanine aminotransferase (ALT) activity. Additionally, ESM-fed mice displayed significantly less aortic root lipid accumulation (−31%) compared to controls. This improvement in atherosclerosis was paired with over a two-fold reduction in circulating serum amyloid A (SAA) in ESM-fed mice. Finally, there was also a modulation of the gut microbiota with ESM supplementation. ESM may have the potential to prevent atherosclerosis, however further research in the clinical setting is warranted. MDPI 2019-05-21 /pmc/articles/PMC6566691/ /pubmed/31117179 http://dx.doi.org/10.3390/nu11051124 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Millar, Courtney L. Norris, Gregory H. Vitols, Addison Garcia, Chelsea Seibel, Samantha Anto, Liya Blesso, Christopher N. Dietary Egg Sphingomyelin Prevents Aortic Root Plaque Accumulation in Apolipoprotein-E Knockout Mice |
title | Dietary Egg Sphingomyelin Prevents Aortic Root Plaque Accumulation in Apolipoprotein-E Knockout Mice |
title_full | Dietary Egg Sphingomyelin Prevents Aortic Root Plaque Accumulation in Apolipoprotein-E Knockout Mice |
title_fullStr | Dietary Egg Sphingomyelin Prevents Aortic Root Plaque Accumulation in Apolipoprotein-E Knockout Mice |
title_full_unstemmed | Dietary Egg Sphingomyelin Prevents Aortic Root Plaque Accumulation in Apolipoprotein-E Knockout Mice |
title_short | Dietary Egg Sphingomyelin Prevents Aortic Root Plaque Accumulation in Apolipoprotein-E Knockout Mice |
title_sort | dietary egg sphingomyelin prevents aortic root plaque accumulation in apolipoprotein-e knockout mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6566691/ https://www.ncbi.nlm.nih.gov/pubmed/31117179 http://dx.doi.org/10.3390/nu11051124 |
work_keys_str_mv | AT millarcourtneyl dietaryeggsphingomyelinpreventsaorticrootplaqueaccumulationinapolipoproteineknockoutmice AT norrisgregoryh dietaryeggsphingomyelinpreventsaorticrootplaqueaccumulationinapolipoproteineknockoutmice AT vitolsaddison dietaryeggsphingomyelinpreventsaorticrootplaqueaccumulationinapolipoproteineknockoutmice AT garciachelsea dietaryeggsphingomyelinpreventsaorticrootplaqueaccumulationinapolipoproteineknockoutmice AT seibelsamantha dietaryeggsphingomyelinpreventsaorticrootplaqueaccumulationinapolipoproteineknockoutmice AT antoliya dietaryeggsphingomyelinpreventsaorticrootplaqueaccumulationinapolipoproteineknockoutmice AT blessochristophern dietaryeggsphingomyelinpreventsaorticrootplaqueaccumulationinapolipoproteineknockoutmice |