Cargando…

Physiological and Proteomic Responses of Mulberry Trees (Morus alba. L.) to Combined Salt and Drought Stress

Intensive investigations have been conducted on the effect of sole drought or salinity stress on the growth of plants. However, there is relatively little knowledge on how plants, particularly woody species, respond to a combination of these two stresses although these stresses can simultaneously oc...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Yan, Ji, Dongfeng, Turgeon, Robert, Chen, Jine, Lin, Tianbao, Huang, Jing, Luo, Jie, Zhu, Yan, Zhang, Cankui, Lv, Zhiqiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6566768/
https://www.ncbi.nlm.nih.gov/pubmed/31137512
http://dx.doi.org/10.3390/ijms20102486
_version_ 1783426924497862656
author Liu, Yan
Ji, Dongfeng
Turgeon, Robert
Chen, Jine
Lin, Tianbao
Huang, Jing
Luo, Jie
Zhu, Yan
Zhang, Cankui
Lv, Zhiqiang
author_facet Liu, Yan
Ji, Dongfeng
Turgeon, Robert
Chen, Jine
Lin, Tianbao
Huang, Jing
Luo, Jie
Zhu, Yan
Zhang, Cankui
Lv, Zhiqiang
author_sort Liu, Yan
collection PubMed
description Intensive investigations have been conducted on the effect of sole drought or salinity stress on the growth of plants. However, there is relatively little knowledge on how plants, particularly woody species, respond to a combination of these two stresses although these stresses can simultaneously occur in the field. In this study, mulberry, an economically important resource for traditional medicine, and the sole food of domesticated silkworms was subjected to a combination of salt and drought stress and analyzed by physiological methods and TMT-based proteomics. Stressed mulberry exhibited significant alteration in physiological parameters, including root/shoot ratio, chlorophyll fluorescence, total carbon, and ion reallocation. A total of 577 and 270 differentially expressed proteins (DEPs) were identified from the stressed leaves and roots, respectively. Through KEGG analysis, these DEPs were assigned to multiple pathways, including carbon metabolism, photosynthesis, redox, secondary metabolism, and hormone metabolism. Among these pathways, the sucrose related metabolic pathway was distinctly enriched in both stressed leaves and roots, indicating an important contribution in mulberry under stress condition. The results provide a comprehensive understanding of the adaptive mechanism of mulberry in response to salt and drought stress, which will facilitate further studies on innovations in terms of crop performance.
format Online
Article
Text
id pubmed-6566768
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-65667682019-06-17 Physiological and Proteomic Responses of Mulberry Trees (Morus alba. L.) to Combined Salt and Drought Stress Liu, Yan Ji, Dongfeng Turgeon, Robert Chen, Jine Lin, Tianbao Huang, Jing Luo, Jie Zhu, Yan Zhang, Cankui Lv, Zhiqiang Int J Mol Sci Article Intensive investigations have been conducted on the effect of sole drought or salinity stress on the growth of plants. However, there is relatively little knowledge on how plants, particularly woody species, respond to a combination of these two stresses although these stresses can simultaneously occur in the field. In this study, mulberry, an economically important resource for traditional medicine, and the sole food of domesticated silkworms was subjected to a combination of salt and drought stress and analyzed by physiological methods and TMT-based proteomics. Stressed mulberry exhibited significant alteration in physiological parameters, including root/shoot ratio, chlorophyll fluorescence, total carbon, and ion reallocation. A total of 577 and 270 differentially expressed proteins (DEPs) were identified from the stressed leaves and roots, respectively. Through KEGG analysis, these DEPs were assigned to multiple pathways, including carbon metabolism, photosynthesis, redox, secondary metabolism, and hormone metabolism. Among these pathways, the sucrose related metabolic pathway was distinctly enriched in both stressed leaves and roots, indicating an important contribution in mulberry under stress condition. The results provide a comprehensive understanding of the adaptive mechanism of mulberry in response to salt and drought stress, which will facilitate further studies on innovations in terms of crop performance. MDPI 2019-05-20 /pmc/articles/PMC6566768/ /pubmed/31137512 http://dx.doi.org/10.3390/ijms20102486 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Yan
Ji, Dongfeng
Turgeon, Robert
Chen, Jine
Lin, Tianbao
Huang, Jing
Luo, Jie
Zhu, Yan
Zhang, Cankui
Lv, Zhiqiang
Physiological and Proteomic Responses of Mulberry Trees (Morus alba. L.) to Combined Salt and Drought Stress
title Physiological and Proteomic Responses of Mulberry Trees (Morus alba. L.) to Combined Salt and Drought Stress
title_full Physiological and Proteomic Responses of Mulberry Trees (Morus alba. L.) to Combined Salt and Drought Stress
title_fullStr Physiological and Proteomic Responses of Mulberry Trees (Morus alba. L.) to Combined Salt and Drought Stress
title_full_unstemmed Physiological and Proteomic Responses of Mulberry Trees (Morus alba. L.) to Combined Salt and Drought Stress
title_short Physiological and Proteomic Responses of Mulberry Trees (Morus alba. L.) to Combined Salt and Drought Stress
title_sort physiological and proteomic responses of mulberry trees (morus alba. l.) to combined salt and drought stress
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6566768/
https://www.ncbi.nlm.nih.gov/pubmed/31137512
http://dx.doi.org/10.3390/ijms20102486
work_keys_str_mv AT liuyan physiologicalandproteomicresponsesofmulberrytreesmorusalbaltocombinedsaltanddroughtstress
AT jidongfeng physiologicalandproteomicresponsesofmulberrytreesmorusalbaltocombinedsaltanddroughtstress
AT turgeonrobert physiologicalandproteomicresponsesofmulberrytreesmorusalbaltocombinedsaltanddroughtstress
AT chenjine physiologicalandproteomicresponsesofmulberrytreesmorusalbaltocombinedsaltanddroughtstress
AT lintianbao physiologicalandproteomicresponsesofmulberrytreesmorusalbaltocombinedsaltanddroughtstress
AT huangjing physiologicalandproteomicresponsesofmulberrytreesmorusalbaltocombinedsaltanddroughtstress
AT luojie physiologicalandproteomicresponsesofmulberrytreesmorusalbaltocombinedsaltanddroughtstress
AT zhuyan physiologicalandproteomicresponsesofmulberrytreesmorusalbaltocombinedsaltanddroughtstress
AT zhangcankui physiologicalandproteomicresponsesofmulberrytreesmorusalbaltocombinedsaltanddroughtstress
AT lvzhiqiang physiologicalandproteomicresponsesofmulberrytreesmorusalbaltocombinedsaltanddroughtstress