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Wearable Enzymatic Alcohol Biosensor

Transdermal alcohol biosensors have the ability to detect the alcohol that emanates from the bloodstream and diffuses through the skin. However, previous biosensors have suffered from long-term fouling of the sensor element and drift in the resulting sensor readings over time. Here, we report a wear...

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Autores principales: Lansdorp, Bob, Ramsay, William, Hamid, Rashad, Strenk, Evan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6566815/
https://www.ncbi.nlm.nih.gov/pubmed/31137611
http://dx.doi.org/10.3390/s19102380
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author Lansdorp, Bob
Ramsay, William
Hamid, Rashad
Strenk, Evan
author_facet Lansdorp, Bob
Ramsay, William
Hamid, Rashad
Strenk, Evan
author_sort Lansdorp, Bob
collection PubMed
description Transdermal alcohol biosensors have the ability to detect the alcohol that emanates from the bloodstream and diffuses through the skin. However, previous biosensors have suffered from long-term fouling of the sensor element and drift in the resulting sensor readings over time. Here, we report a wearable alcohol sensor platform that solves the problem of sensor fouling by enabling drift-free signals in vivo for up to 24 h and an interchangeable cartridge connection that enables consecutive days of measurement. We demonstrate how alcohol oxidase enzyme and Prussian Blue can be combined to prevent baseline drift above 25 nA, enabling sensitive detection of transdermal alcohol. Laboratory characterization of the enzymatic alcohol sensor demonstrates that the sensor is mass-transfer-limited by a diffusion-limiting membrane of lower permeability than human skin and a linear sensor range between 0 mM and 50 mM. Further, we show continuous transdermal alcohol data recorded with a human subject for two consecutive days. The non-invasive sensor presented here is an objective alternative to the self-reports used commonly to quantify alcohol consumption in research studies.
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spelling pubmed-65668152019-06-17 Wearable Enzymatic Alcohol Biosensor Lansdorp, Bob Ramsay, William Hamid, Rashad Strenk, Evan Sensors (Basel) Article Transdermal alcohol biosensors have the ability to detect the alcohol that emanates from the bloodstream and diffuses through the skin. However, previous biosensors have suffered from long-term fouling of the sensor element and drift in the resulting sensor readings over time. Here, we report a wearable alcohol sensor platform that solves the problem of sensor fouling by enabling drift-free signals in vivo for up to 24 h and an interchangeable cartridge connection that enables consecutive days of measurement. We demonstrate how alcohol oxidase enzyme and Prussian Blue can be combined to prevent baseline drift above 25 nA, enabling sensitive detection of transdermal alcohol. Laboratory characterization of the enzymatic alcohol sensor demonstrates that the sensor is mass-transfer-limited by a diffusion-limiting membrane of lower permeability than human skin and a linear sensor range between 0 mM and 50 mM. Further, we show continuous transdermal alcohol data recorded with a human subject for two consecutive days. The non-invasive sensor presented here is an objective alternative to the self-reports used commonly to quantify alcohol consumption in research studies. MDPI 2019-05-24 /pmc/articles/PMC6566815/ /pubmed/31137611 http://dx.doi.org/10.3390/s19102380 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lansdorp, Bob
Ramsay, William
Hamid, Rashad
Strenk, Evan
Wearable Enzymatic Alcohol Biosensor
title Wearable Enzymatic Alcohol Biosensor
title_full Wearable Enzymatic Alcohol Biosensor
title_fullStr Wearable Enzymatic Alcohol Biosensor
title_full_unstemmed Wearable Enzymatic Alcohol Biosensor
title_short Wearable Enzymatic Alcohol Biosensor
title_sort wearable enzymatic alcohol biosensor
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6566815/
https://www.ncbi.nlm.nih.gov/pubmed/31137611
http://dx.doi.org/10.3390/s19102380
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