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Proteomic Landscape of the Mature Roots in a Rubber-Producing Grass Taraxacum Kok-saghyz
The rubber grass Taraxacum kok-saghyz (TKS) contains large amounts of natural rubber (cis-1,4-polyisoprene) in its enlarged roots and it is an alternative crop source of natural rubber. Natural rubber biosynthesis (NRB) and storage in the mature roots of TKS is a cascade process involving many genes...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6566844/ https://www.ncbi.nlm.nih.gov/pubmed/31137823 http://dx.doi.org/10.3390/ijms20102596 |
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author | Xie, Quanliang Ding, Guohua Zhu, Liping Yu, Li Yuan, Boxuan Gao, Xuan Wang, Dan Sun, Yong Liu, Yang Li, Hongbin Wang, Xuchu |
author_facet | Xie, Quanliang Ding, Guohua Zhu, Liping Yu, Li Yuan, Boxuan Gao, Xuan Wang, Dan Sun, Yong Liu, Yang Li, Hongbin Wang, Xuchu |
author_sort | Xie, Quanliang |
collection | PubMed |
description | The rubber grass Taraxacum kok-saghyz (TKS) contains large amounts of natural rubber (cis-1,4-polyisoprene) in its enlarged roots and it is an alternative crop source of natural rubber. Natural rubber biosynthesis (NRB) and storage in the mature roots of TKS is a cascade process involving many genes, proteins and their cofactors. The TKS genome has just been annotated and many NRB-related genes have been determined. However, there is limited knowledge about the protein regulation mechanism for NRB in TKS roots. We identified 371 protein species from the mature roots of TKS by combining two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS). Meanwhile, a large-scale shotgun analysis of proteins in TKS roots at the enlargement stage was performed, and 3545 individual proteins were determined. Subsequently, all identified proteins from 2-DE gel and shotgun MS in TKS roots were subject to gene ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses and most proteins were involved in carbon metabolic process with catalytic activity in membrane-bounded organelles, followed by proteins with binding ability, transportation and phenylpropanoid biosynthesis activities. Fifty-eight NRB-related proteins, including eight small rubber particle protein (SRPP) and two rubber elongation factor(REF) members, were identified from the TKS roots, and these proteins were involved in both mevalonate acid (MVA) and methylerythritol phosphate (MEP) pathways. To our best knowledge, it is the first high-resolution draft proteome map of the mature TKS roots. Our proteomics of TKS roots revealed both MVA and MEP pathways are important for NRB, and SRPP might be more important than REF for NRB in TKS roots. These findings would not only deepen our understanding of the TKS root proteome, but also provide new evidence on the roles of these NRB-related proteins in the mature TKS roots. |
format | Online Article Text |
id | pubmed-6566844 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-65668442019-06-17 Proteomic Landscape of the Mature Roots in a Rubber-Producing Grass Taraxacum Kok-saghyz Xie, Quanliang Ding, Guohua Zhu, Liping Yu, Li Yuan, Boxuan Gao, Xuan Wang, Dan Sun, Yong Liu, Yang Li, Hongbin Wang, Xuchu Int J Mol Sci Article The rubber grass Taraxacum kok-saghyz (TKS) contains large amounts of natural rubber (cis-1,4-polyisoprene) in its enlarged roots and it is an alternative crop source of natural rubber. Natural rubber biosynthesis (NRB) and storage in the mature roots of TKS is a cascade process involving many genes, proteins and their cofactors. The TKS genome has just been annotated and many NRB-related genes have been determined. However, there is limited knowledge about the protein regulation mechanism for NRB in TKS roots. We identified 371 protein species from the mature roots of TKS by combining two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS). Meanwhile, a large-scale shotgun analysis of proteins in TKS roots at the enlargement stage was performed, and 3545 individual proteins were determined. Subsequently, all identified proteins from 2-DE gel and shotgun MS in TKS roots were subject to gene ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses and most proteins were involved in carbon metabolic process with catalytic activity in membrane-bounded organelles, followed by proteins with binding ability, transportation and phenylpropanoid biosynthesis activities. Fifty-eight NRB-related proteins, including eight small rubber particle protein (SRPP) and two rubber elongation factor(REF) members, were identified from the TKS roots, and these proteins were involved in both mevalonate acid (MVA) and methylerythritol phosphate (MEP) pathways. To our best knowledge, it is the first high-resolution draft proteome map of the mature TKS roots. Our proteomics of TKS roots revealed both MVA and MEP pathways are important for NRB, and SRPP might be more important than REF for NRB in TKS roots. These findings would not only deepen our understanding of the TKS root proteome, but also provide new evidence on the roles of these NRB-related proteins in the mature TKS roots. MDPI 2019-05-27 /pmc/articles/PMC6566844/ /pubmed/31137823 http://dx.doi.org/10.3390/ijms20102596 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Xie, Quanliang Ding, Guohua Zhu, Liping Yu, Li Yuan, Boxuan Gao, Xuan Wang, Dan Sun, Yong Liu, Yang Li, Hongbin Wang, Xuchu Proteomic Landscape of the Mature Roots in a Rubber-Producing Grass Taraxacum Kok-saghyz |
title | Proteomic Landscape of the Mature Roots in a Rubber-Producing Grass Taraxacum Kok-saghyz |
title_full | Proteomic Landscape of the Mature Roots in a Rubber-Producing Grass Taraxacum Kok-saghyz |
title_fullStr | Proteomic Landscape of the Mature Roots in a Rubber-Producing Grass Taraxacum Kok-saghyz |
title_full_unstemmed | Proteomic Landscape of the Mature Roots in a Rubber-Producing Grass Taraxacum Kok-saghyz |
title_short | Proteomic Landscape of the Mature Roots in a Rubber-Producing Grass Taraxacum Kok-saghyz |
title_sort | proteomic landscape of the mature roots in a rubber-producing grass taraxacum kok-saghyz |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6566844/ https://www.ncbi.nlm.nih.gov/pubmed/31137823 http://dx.doi.org/10.3390/ijms20102596 |
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