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Biomimetic Collagen/Zn(2+)-Substituted Calcium Phosphate Composite Coatings on Titanium Substrates as Prospective Bioactive Layer for Implants: A Comparative Study Spin Coating vs. MAPLE
Synthesis of biomimetic materials for implants and prostheses is a hot topic in nanobiotechnology strategies. Today the major approach of orthopaedic implants in hard tissue engineering is represented by titanium implants. A comparative study of hybrid thin coatings deposition was performed by spin...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6566990/ https://www.ncbi.nlm.nih.gov/pubmed/31058851 http://dx.doi.org/10.3390/nano9050692 |
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author | Neacsu, Ionela Andreea Arsenie, Laura Vasilica Trusca, Roxana Ardelean, Ioana Lavinia Mihailescu, Natalia Mihailescu, Ion Nicolae Ristoscu, Carmen Bleotu, Coralia Ficai, Anton Andronescu, Ecaterina |
author_facet | Neacsu, Ionela Andreea Arsenie, Laura Vasilica Trusca, Roxana Ardelean, Ioana Lavinia Mihailescu, Natalia Mihailescu, Ion Nicolae Ristoscu, Carmen Bleotu, Coralia Ficai, Anton Andronescu, Ecaterina |
author_sort | Neacsu, Ionela Andreea |
collection | PubMed |
description | Synthesis of biomimetic materials for implants and prostheses is a hot topic in nanobiotechnology strategies. Today the major approach of orthopaedic implants in hard tissue engineering is represented by titanium implants. A comparative study of hybrid thin coatings deposition was performed by spin coating and matrix-assisted pulsed laser evaporation (MAPLE) onto titanium substrates. The Collagen-calcium phosphate (Coll-CaPs) combination was selected as the best option to mimic natural bone tissue. To accelerate the mineralization process, Zn(2+) ions were inserted by substitution in CaPs. A superior thin film homogeneity was assessed by MAPLE, as shown by scanning electron microscopy (SEM) and Fourier transform infrared (FTIR) microscopy. A decrease of P-O and amide absorbance bands was observed as a consequence of different Zn(2+) amounts. A variety of structural modifications of the apatite layer are then generated, which influenced the confinement process towards the collagen template. The in-vitro Simulated Body Fluid (SBF) assay demonstrated the ability of Coll/Zn(2+)-CaPs coatings to stimulate the mineralization process as a result of synergic effects in the collagen-Zn(2+) substituted apatite. For both deposition methods, the formation of droplets associated to the growth of CaPs particulates inside the collagen matrix was visualized. This supports the prospective behavior of MAPLE biomimetic coatings to induce mineralization, as an essential step of fast implant integration with vivid tissues. |
format | Online Article Text |
id | pubmed-6566990 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-65669902019-06-17 Biomimetic Collagen/Zn(2+)-Substituted Calcium Phosphate Composite Coatings on Titanium Substrates as Prospective Bioactive Layer for Implants: A Comparative Study Spin Coating vs. MAPLE Neacsu, Ionela Andreea Arsenie, Laura Vasilica Trusca, Roxana Ardelean, Ioana Lavinia Mihailescu, Natalia Mihailescu, Ion Nicolae Ristoscu, Carmen Bleotu, Coralia Ficai, Anton Andronescu, Ecaterina Nanomaterials (Basel) Article Synthesis of biomimetic materials for implants and prostheses is a hot topic in nanobiotechnology strategies. Today the major approach of orthopaedic implants in hard tissue engineering is represented by titanium implants. A comparative study of hybrid thin coatings deposition was performed by spin coating and matrix-assisted pulsed laser evaporation (MAPLE) onto titanium substrates. The Collagen-calcium phosphate (Coll-CaPs) combination was selected as the best option to mimic natural bone tissue. To accelerate the mineralization process, Zn(2+) ions were inserted by substitution in CaPs. A superior thin film homogeneity was assessed by MAPLE, as shown by scanning electron microscopy (SEM) and Fourier transform infrared (FTIR) microscopy. A decrease of P-O and amide absorbance bands was observed as a consequence of different Zn(2+) amounts. A variety of structural modifications of the apatite layer are then generated, which influenced the confinement process towards the collagen template. The in-vitro Simulated Body Fluid (SBF) assay demonstrated the ability of Coll/Zn(2+)-CaPs coatings to stimulate the mineralization process as a result of synergic effects in the collagen-Zn(2+) substituted apatite. For both deposition methods, the formation of droplets associated to the growth of CaPs particulates inside the collagen matrix was visualized. This supports the prospective behavior of MAPLE biomimetic coatings to induce mineralization, as an essential step of fast implant integration with vivid tissues. MDPI 2019-05-03 /pmc/articles/PMC6566990/ /pubmed/31058851 http://dx.doi.org/10.3390/nano9050692 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Neacsu, Ionela Andreea Arsenie, Laura Vasilica Trusca, Roxana Ardelean, Ioana Lavinia Mihailescu, Natalia Mihailescu, Ion Nicolae Ristoscu, Carmen Bleotu, Coralia Ficai, Anton Andronescu, Ecaterina Biomimetic Collagen/Zn(2+)-Substituted Calcium Phosphate Composite Coatings on Titanium Substrates as Prospective Bioactive Layer for Implants: A Comparative Study Spin Coating vs. MAPLE |
title | Biomimetic Collagen/Zn(2+)-Substituted Calcium Phosphate Composite Coatings on Titanium Substrates as Prospective Bioactive Layer for Implants: A Comparative Study Spin Coating vs. MAPLE |
title_full | Biomimetic Collagen/Zn(2+)-Substituted Calcium Phosphate Composite Coatings on Titanium Substrates as Prospective Bioactive Layer for Implants: A Comparative Study Spin Coating vs. MAPLE |
title_fullStr | Biomimetic Collagen/Zn(2+)-Substituted Calcium Phosphate Composite Coatings on Titanium Substrates as Prospective Bioactive Layer for Implants: A Comparative Study Spin Coating vs. MAPLE |
title_full_unstemmed | Biomimetic Collagen/Zn(2+)-Substituted Calcium Phosphate Composite Coatings on Titanium Substrates as Prospective Bioactive Layer for Implants: A Comparative Study Spin Coating vs. MAPLE |
title_short | Biomimetic Collagen/Zn(2+)-Substituted Calcium Phosphate Composite Coatings on Titanium Substrates as Prospective Bioactive Layer for Implants: A Comparative Study Spin Coating vs. MAPLE |
title_sort | biomimetic collagen/zn(2+)-substituted calcium phosphate composite coatings on titanium substrates as prospective bioactive layer for implants: a comparative study spin coating vs. maple |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6566990/ https://www.ncbi.nlm.nih.gov/pubmed/31058851 http://dx.doi.org/10.3390/nano9050692 |
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