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Therapeutic Potential of a Combination of Magnesium Hydroxide Nanoparticles and Sericin for Epithelial Corneal Wound Healing

We previously found the instillation of sericin to be useful as therapy for keratopathy with or without diabetes mellitus. In this study, we investigated whether a combination of solid magnesium hydroxide nanoparticles (MHN) enhances epithelial corneal wound healing by sericin using rabbits, normal...

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Autores principales: Nagai, Noriaki, Iwai, Yoshie, Deguchi, Saori, Otake, Hiroko, Kanai, Kazutaka, Okamoto, Norio, Shimomura, Yoshikazu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6567023/
https://www.ncbi.nlm.nih.gov/pubmed/31109118
http://dx.doi.org/10.3390/nano9050768
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author Nagai, Noriaki
Iwai, Yoshie
Deguchi, Saori
Otake, Hiroko
Kanai, Kazutaka
Okamoto, Norio
Shimomura, Yoshikazu
author_facet Nagai, Noriaki
Iwai, Yoshie
Deguchi, Saori
Otake, Hiroko
Kanai, Kazutaka
Okamoto, Norio
Shimomura, Yoshikazu
author_sort Nagai, Noriaki
collection PubMed
description We previously found the instillation of sericin to be useful as therapy for keratopathy with or without diabetes mellitus. In this study, we investigated whether a combination of solid magnesium hydroxide nanoparticles (MHN) enhances epithelial corneal wound healing by sericin using rabbits, normal rats and type 2 diabetes mellitus rats with debrided corneal epithelium (ex vivo and in vivo studies). Ophthalmic formulations containing sericin and MHN (N-Ser) were prepared using a bead mill method. The mean particle size of the N-Ser was 110.3 nm at the time of preparation, and 148.1 nm one month later. The instillation of N-Ser had no effect on the amount of lacrimal fluid in normal rabbits (in vivo), but the MHN in N-Ser was found to expand the intercellular space in ex vivo rat corneas. In addition, the instillation of N-Ser increased the phosphorylation of Extracellular Signal-regulated Kinase (ERK)1/2, a factor involved in cell adhesion and cell proliferation in the corneal epithelium, in comparison with the instillation of sericin alone. The combination with MHN enhanced epithelial corneal wound healing by sericin in rat debrided corneal epithelium (in vivo). This study provides significant information to prepare potent drugs to cure severe keratopathy, such as diabetic keratopathy.
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spelling pubmed-65670232019-06-17 Therapeutic Potential of a Combination of Magnesium Hydroxide Nanoparticles and Sericin for Epithelial Corneal Wound Healing Nagai, Noriaki Iwai, Yoshie Deguchi, Saori Otake, Hiroko Kanai, Kazutaka Okamoto, Norio Shimomura, Yoshikazu Nanomaterials (Basel) Article We previously found the instillation of sericin to be useful as therapy for keratopathy with or without diabetes mellitus. In this study, we investigated whether a combination of solid magnesium hydroxide nanoparticles (MHN) enhances epithelial corneal wound healing by sericin using rabbits, normal rats and type 2 diabetes mellitus rats with debrided corneal epithelium (ex vivo and in vivo studies). Ophthalmic formulations containing sericin and MHN (N-Ser) were prepared using a bead mill method. The mean particle size of the N-Ser was 110.3 nm at the time of preparation, and 148.1 nm one month later. The instillation of N-Ser had no effect on the amount of lacrimal fluid in normal rabbits (in vivo), but the MHN in N-Ser was found to expand the intercellular space in ex vivo rat corneas. In addition, the instillation of N-Ser increased the phosphorylation of Extracellular Signal-regulated Kinase (ERK)1/2, a factor involved in cell adhesion and cell proliferation in the corneal epithelium, in comparison with the instillation of sericin alone. The combination with MHN enhanced epithelial corneal wound healing by sericin in rat debrided corneal epithelium (in vivo). This study provides significant information to prepare potent drugs to cure severe keratopathy, such as diabetic keratopathy. MDPI 2019-05-19 /pmc/articles/PMC6567023/ /pubmed/31109118 http://dx.doi.org/10.3390/nano9050768 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nagai, Noriaki
Iwai, Yoshie
Deguchi, Saori
Otake, Hiroko
Kanai, Kazutaka
Okamoto, Norio
Shimomura, Yoshikazu
Therapeutic Potential of a Combination of Magnesium Hydroxide Nanoparticles and Sericin for Epithelial Corneal Wound Healing
title Therapeutic Potential of a Combination of Magnesium Hydroxide Nanoparticles and Sericin for Epithelial Corneal Wound Healing
title_full Therapeutic Potential of a Combination of Magnesium Hydroxide Nanoparticles and Sericin for Epithelial Corneal Wound Healing
title_fullStr Therapeutic Potential of a Combination of Magnesium Hydroxide Nanoparticles and Sericin for Epithelial Corneal Wound Healing
title_full_unstemmed Therapeutic Potential of a Combination of Magnesium Hydroxide Nanoparticles and Sericin for Epithelial Corneal Wound Healing
title_short Therapeutic Potential of a Combination of Magnesium Hydroxide Nanoparticles and Sericin for Epithelial Corneal Wound Healing
title_sort therapeutic potential of a combination of magnesium hydroxide nanoparticles and sericin for epithelial corneal wound healing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6567023/
https://www.ncbi.nlm.nih.gov/pubmed/31109118
http://dx.doi.org/10.3390/nano9050768
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