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Nanomelanin Potentially Protects the Spleen from Radiotherapy-Associated Damage and Enhances Immunoactivity in Tumor-Bearing Mice

Radiotherapy side-effects present serious problems in cancer treatment. Melanin, a natural polymer with low toxicity, is considered as a potential radio-protector; however, its application as an agent against irradiation during cancer treatment has still received little attention. In this study, nan...

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Autores principales: Le Na, Nguyen Thi, Duc Loc, Sai, Minh Tri, Nguyen Le, Bich Loan, Nguyen Thi, Anh Son, Ho, Linh Toan, Nguyen, Phuong Thu, Ha, My Nhung, Hoang Thi, Lai Thanh, Nguyen, Van Anh, Nguyen Thi, Dinh Thang, Nguyen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6567087/
https://www.ncbi.nlm.nih.gov/pubmed/31137873
http://dx.doi.org/10.3390/ma12101725
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author Le Na, Nguyen Thi
Duc Loc, Sai
Minh Tri, Nguyen Le
Bich Loan, Nguyen Thi
Anh Son, Ho
Linh Toan, Nguyen
Phuong Thu, Ha
My Nhung, Hoang Thi
Lai Thanh, Nguyen
Van Anh, Nguyen Thi
Dinh Thang, Nguyen
author_facet Le Na, Nguyen Thi
Duc Loc, Sai
Minh Tri, Nguyen Le
Bich Loan, Nguyen Thi
Anh Son, Ho
Linh Toan, Nguyen
Phuong Thu, Ha
My Nhung, Hoang Thi
Lai Thanh, Nguyen
Van Anh, Nguyen Thi
Dinh Thang, Nguyen
author_sort Le Na, Nguyen Thi
collection PubMed
description Radiotherapy side-effects present serious problems in cancer treatment. Melanin, a natural polymer with low toxicity, is considered as a potential radio-protector; however, its application as an agent against irradiation during cancer treatment has still received little attention. In this study, nanomelanin particles were prepared, characterized and applied in protecting the spleens of tumor-bearing mice irradiated with X-rays. These nanoparticles had sizes varying in the range of 80–200 nm and contained several important functional groups such as carboxyl (-COO), carbonyl (-C=O) and hydroxyl (-OH) groups on the surfaces. Tumor-bearing mice were treated with nanomelanin at a concentration of 40 mg/kg before irradiating with a single dose of 6.0 Gray of X-ray at a high dose rate (1.0 Gray/min). Impressively, X-ray caused mild splenic fibrosis in 40% of nanomelanin-protected mice, whereas severe fibrosis was observed in 100% of mice treated with X-ray alone. Treatment with nanomelanin also partly rescued the volume and weight of mouse spleens from irradiation through promoting the transcription levels of splenic Interleukin-2 (IL-2) and Tumor Necrosis Factor alpha (TNF-α). More interestingly, splenic T cell and dendritic cell populations were 1.91 and 1.64-fold higher in nanomelanin-treated mice than those in mice which received X-ray alone. Consistently, the percentage of lymphocytes was also significantly greater in blood from nanomelanin-treated mice. In addition, nanomelanin might indirectly induce apoptosis in tumor tissues via activation of TNF-α, Bax, and Caspase-3 genes. In summary, our results demonstrate that nanomelanin protects spleens from X-ray irradiation and consequently enhances immunoactivity in tumor-bearing mice; therefore, we present nanomelanin as a potential protector against damage from radiotherapy in cancer treatment.
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spelling pubmed-65670872019-06-17 Nanomelanin Potentially Protects the Spleen from Radiotherapy-Associated Damage and Enhances Immunoactivity in Tumor-Bearing Mice Le Na, Nguyen Thi Duc Loc, Sai Minh Tri, Nguyen Le Bich Loan, Nguyen Thi Anh Son, Ho Linh Toan, Nguyen Phuong Thu, Ha My Nhung, Hoang Thi Lai Thanh, Nguyen Van Anh, Nguyen Thi Dinh Thang, Nguyen Materials (Basel) Article Radiotherapy side-effects present serious problems in cancer treatment. Melanin, a natural polymer with low toxicity, is considered as a potential radio-protector; however, its application as an agent against irradiation during cancer treatment has still received little attention. In this study, nanomelanin particles were prepared, characterized and applied in protecting the spleens of tumor-bearing mice irradiated with X-rays. These nanoparticles had sizes varying in the range of 80–200 nm and contained several important functional groups such as carboxyl (-COO), carbonyl (-C=O) and hydroxyl (-OH) groups on the surfaces. Tumor-bearing mice were treated with nanomelanin at a concentration of 40 mg/kg before irradiating with a single dose of 6.0 Gray of X-ray at a high dose rate (1.0 Gray/min). Impressively, X-ray caused mild splenic fibrosis in 40% of nanomelanin-protected mice, whereas severe fibrosis was observed in 100% of mice treated with X-ray alone. Treatment with nanomelanin also partly rescued the volume and weight of mouse spleens from irradiation through promoting the transcription levels of splenic Interleukin-2 (IL-2) and Tumor Necrosis Factor alpha (TNF-α). More interestingly, splenic T cell and dendritic cell populations were 1.91 and 1.64-fold higher in nanomelanin-treated mice than those in mice which received X-ray alone. Consistently, the percentage of lymphocytes was also significantly greater in blood from nanomelanin-treated mice. In addition, nanomelanin might indirectly induce apoptosis in tumor tissues via activation of TNF-α, Bax, and Caspase-3 genes. In summary, our results demonstrate that nanomelanin protects spleens from X-ray irradiation and consequently enhances immunoactivity in tumor-bearing mice; therefore, we present nanomelanin as a potential protector against damage from radiotherapy in cancer treatment. MDPI 2019-05-27 /pmc/articles/PMC6567087/ /pubmed/31137873 http://dx.doi.org/10.3390/ma12101725 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Le Na, Nguyen Thi
Duc Loc, Sai
Minh Tri, Nguyen Le
Bich Loan, Nguyen Thi
Anh Son, Ho
Linh Toan, Nguyen
Phuong Thu, Ha
My Nhung, Hoang Thi
Lai Thanh, Nguyen
Van Anh, Nguyen Thi
Dinh Thang, Nguyen
Nanomelanin Potentially Protects the Spleen from Radiotherapy-Associated Damage and Enhances Immunoactivity in Tumor-Bearing Mice
title Nanomelanin Potentially Protects the Spleen from Radiotherapy-Associated Damage and Enhances Immunoactivity in Tumor-Bearing Mice
title_full Nanomelanin Potentially Protects the Spleen from Radiotherapy-Associated Damage and Enhances Immunoactivity in Tumor-Bearing Mice
title_fullStr Nanomelanin Potentially Protects the Spleen from Radiotherapy-Associated Damage and Enhances Immunoactivity in Tumor-Bearing Mice
title_full_unstemmed Nanomelanin Potentially Protects the Spleen from Radiotherapy-Associated Damage and Enhances Immunoactivity in Tumor-Bearing Mice
title_short Nanomelanin Potentially Protects the Spleen from Radiotherapy-Associated Damage and Enhances Immunoactivity in Tumor-Bearing Mice
title_sort nanomelanin potentially protects the spleen from radiotherapy-associated damage and enhances immunoactivity in tumor-bearing mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6567087/
https://www.ncbi.nlm.nih.gov/pubmed/31137873
http://dx.doi.org/10.3390/ma12101725
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