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Adipokinome Signatures in Obese Mouse Models Reflect Adipose Tissue Health and Are Associated with Serum Lipid Composition

Adipocyte and hepatic lipid metabolism govern whole-body metabolic homeostasis, whereas a disbalance of de novo lipogenesis (DNL) in fat and liver might lead to obesity, with severe co-morbidities. Nevertheless, some obese people are metabolically healthy, but the “protective” mechanisms are not yet...

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Autores principales: Knebel, Birgit, Fahlbusch, Pia, Poschmann, Gereon, Dille, Matthias, Wahlers, Natalie, Stühler, Kai, Hartwig, Sonja, Lehr, Stefan, Schiller, Martina, Jacob, Sylvia, Kettel, Ulrike, Müller-Wieland, Dirk, Kotzka, Jörg
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6567124/
https://www.ncbi.nlm.nih.gov/pubmed/31137678
http://dx.doi.org/10.3390/ijms20102559
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author Knebel, Birgit
Fahlbusch, Pia
Poschmann, Gereon
Dille, Matthias
Wahlers, Natalie
Stühler, Kai
Hartwig, Sonja
Lehr, Stefan
Schiller, Martina
Jacob, Sylvia
Kettel, Ulrike
Müller-Wieland, Dirk
Kotzka, Jörg
author_facet Knebel, Birgit
Fahlbusch, Pia
Poschmann, Gereon
Dille, Matthias
Wahlers, Natalie
Stühler, Kai
Hartwig, Sonja
Lehr, Stefan
Schiller, Martina
Jacob, Sylvia
Kettel, Ulrike
Müller-Wieland, Dirk
Kotzka, Jörg
author_sort Knebel, Birgit
collection PubMed
description Adipocyte and hepatic lipid metabolism govern whole-body metabolic homeostasis, whereas a disbalance of de novo lipogenesis (DNL) in fat and liver might lead to obesity, with severe co-morbidities. Nevertheless, some obese people are metabolically healthy, but the “protective” mechanisms are not yet known in detail. Especially, the adipocyte-derived molecular mediators that indicate adipose functionality are poorly understood. We studied transgenic mice (alb-SREBP-1c) with a “healthy” obese phenotype, and obob mice with hyperphagia-induced “sick” obesity to analyze the impact of the tissue-specific DNL on the secreted proteins, i.e., the adipokinome, of the primary adipose cells by label-free proteomics. Compared to the control mice, adipose DNL is reduced in both obese mouse models. In contrast, the hepatic DNL is reduced in obob but elevated in alb-SREBP-1c mice. To investigate the relationship between lipid metabolism and adipokinomes, we formulated the “liver-to-adipose-tissue DNL” ratio. Knowledge-based analyses of these results revealed adipocyte functionality with proteins, which was involved in tissue remodeling or metabolism in the alb-SREBP-1c mice and in the control mice, but mainly in fibrosis in the obob mice. The adipokinome in “healthy” obesity is similar to that in a normal condition, but it differs from that in “sick” obesity, whereas the serum lipid patterns reflect the “liver-to-adipose-tissue DNL” ratio and are associated with the adipokinome signature.
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spelling pubmed-65671242019-06-17 Adipokinome Signatures in Obese Mouse Models Reflect Adipose Tissue Health and Are Associated with Serum Lipid Composition Knebel, Birgit Fahlbusch, Pia Poschmann, Gereon Dille, Matthias Wahlers, Natalie Stühler, Kai Hartwig, Sonja Lehr, Stefan Schiller, Martina Jacob, Sylvia Kettel, Ulrike Müller-Wieland, Dirk Kotzka, Jörg Int J Mol Sci Article Adipocyte and hepatic lipid metabolism govern whole-body metabolic homeostasis, whereas a disbalance of de novo lipogenesis (DNL) in fat and liver might lead to obesity, with severe co-morbidities. Nevertheless, some obese people are metabolically healthy, but the “protective” mechanisms are not yet known in detail. Especially, the adipocyte-derived molecular mediators that indicate adipose functionality are poorly understood. We studied transgenic mice (alb-SREBP-1c) with a “healthy” obese phenotype, and obob mice with hyperphagia-induced “sick” obesity to analyze the impact of the tissue-specific DNL on the secreted proteins, i.e., the adipokinome, of the primary adipose cells by label-free proteomics. Compared to the control mice, adipose DNL is reduced in both obese mouse models. In contrast, the hepatic DNL is reduced in obob but elevated in alb-SREBP-1c mice. To investigate the relationship between lipid metabolism and adipokinomes, we formulated the “liver-to-adipose-tissue DNL” ratio. Knowledge-based analyses of these results revealed adipocyte functionality with proteins, which was involved in tissue remodeling or metabolism in the alb-SREBP-1c mice and in the control mice, but mainly in fibrosis in the obob mice. The adipokinome in “healthy” obesity is similar to that in a normal condition, but it differs from that in “sick” obesity, whereas the serum lipid patterns reflect the “liver-to-adipose-tissue DNL” ratio and are associated with the adipokinome signature. MDPI 2019-05-24 /pmc/articles/PMC6567124/ /pubmed/31137678 http://dx.doi.org/10.3390/ijms20102559 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Knebel, Birgit
Fahlbusch, Pia
Poschmann, Gereon
Dille, Matthias
Wahlers, Natalie
Stühler, Kai
Hartwig, Sonja
Lehr, Stefan
Schiller, Martina
Jacob, Sylvia
Kettel, Ulrike
Müller-Wieland, Dirk
Kotzka, Jörg
Adipokinome Signatures in Obese Mouse Models Reflect Adipose Tissue Health and Are Associated with Serum Lipid Composition
title Adipokinome Signatures in Obese Mouse Models Reflect Adipose Tissue Health and Are Associated with Serum Lipid Composition
title_full Adipokinome Signatures in Obese Mouse Models Reflect Adipose Tissue Health and Are Associated with Serum Lipid Composition
title_fullStr Adipokinome Signatures in Obese Mouse Models Reflect Adipose Tissue Health and Are Associated with Serum Lipid Composition
title_full_unstemmed Adipokinome Signatures in Obese Mouse Models Reflect Adipose Tissue Health and Are Associated with Serum Lipid Composition
title_short Adipokinome Signatures in Obese Mouse Models Reflect Adipose Tissue Health and Are Associated with Serum Lipid Composition
title_sort adipokinome signatures in obese mouse models reflect adipose tissue health and are associated with serum lipid composition
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6567124/
https://www.ncbi.nlm.nih.gov/pubmed/31137678
http://dx.doi.org/10.3390/ijms20102559
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