Cargando…
Molecular Responses in THP-1 Macrophage-Like Cells Exposed to Diverse Nanoparticles
In the body, engineered nanoparticles (NPs) may be recognized and processed by immune cells, among which macrophages play a crucial role. We evaluated the effects of selected NPs [NM-100 (TiO(2)), NM-110 (ZnO), NM-200 (SiO(2)), and NM-300 K (Ag)] on THP-1 macrophage-like cells. The cells were expose...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6567235/ https://www.ncbi.nlm.nih.gov/pubmed/31052583 http://dx.doi.org/10.3390/nano9050687 |
_version_ | 1783427030042279936 |
---|---|
author | Brzicova, Tana Javorkova, Eliska Vrbova, Kristyna Zajicova, Alena Holan, Vladimir Pinkas, Dominik Philimonenko, Vlada Sikorova, Jitka Klema, Jiri Topinka, Jan Rossner, Pavel |
author_facet | Brzicova, Tana Javorkova, Eliska Vrbova, Kristyna Zajicova, Alena Holan, Vladimir Pinkas, Dominik Philimonenko, Vlada Sikorova, Jitka Klema, Jiri Topinka, Jan Rossner, Pavel |
author_sort | Brzicova, Tana |
collection | PubMed |
description | In the body, engineered nanoparticles (NPs) may be recognized and processed by immune cells, among which macrophages play a crucial role. We evaluated the effects of selected NPs [NM-100 (TiO(2)), NM-110 (ZnO), NM-200 (SiO(2)), and NM-300 K (Ag)] on THP-1 macrophage-like cells. The cells were exposed to subcytotoxic concentrations of NPs (1–25 µg/mL) and the expression of immunologically relevant genes (VCAM1, TNFA, CXCL8, ICAM1, CD86, CD192, and IL1B) was analyzed by RT-qPCR. The expression of selected cytokines, growth factors and surface molecules was assessed by flow cytometry or ELISA. Generation of reactive oxygen species and induction of DNA breaks were also analyzed. Exposure to diverse NPs caused substantially different molecular responses. No significant effects were detected for NM-100 treatment. NM-200 induced production of IL-8, a potent attractor and activator of neutrophils, growth factors (VEGF and IGF-1) and superoxide. NM-110 triggered a proinflammatory response, characterized by the activation of transcription factor NF-κB, an enhanced production of proinflammatory cytokines (TNF-α) and chemokines (IL-8). Furthermore, the expression of cell adhesion molecules VCAM-1 and ICAM-1 and hepatocyte growth factor (HGF), as well as superoxide production and DNA breaks, were affected. NM-300 K enhanced IL-8 production and induced DNA breaks, however, it decreased the expression of chemokine receptor (CCR2) and CD86 molecule, indicating potential immunosuppressive activity. The toxicity of ZnO and Ag NPs was probably caused by their intracellular dissolution, as indicated by transmission electron microscopy imaging. The observed effects in macrophages might further influence both innate and adaptive immune responses by promoting neutrophil recruitment via IL-8 release and enhancing the adhesion and stimulation of T cells by VCAM-1 and ICAM-1 expression. |
format | Online Article Text |
id | pubmed-6567235 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-65672352019-06-17 Molecular Responses in THP-1 Macrophage-Like Cells Exposed to Diverse Nanoparticles Brzicova, Tana Javorkova, Eliska Vrbova, Kristyna Zajicova, Alena Holan, Vladimir Pinkas, Dominik Philimonenko, Vlada Sikorova, Jitka Klema, Jiri Topinka, Jan Rossner, Pavel Nanomaterials (Basel) Article In the body, engineered nanoparticles (NPs) may be recognized and processed by immune cells, among which macrophages play a crucial role. We evaluated the effects of selected NPs [NM-100 (TiO(2)), NM-110 (ZnO), NM-200 (SiO(2)), and NM-300 K (Ag)] on THP-1 macrophage-like cells. The cells were exposed to subcytotoxic concentrations of NPs (1–25 µg/mL) and the expression of immunologically relevant genes (VCAM1, TNFA, CXCL8, ICAM1, CD86, CD192, and IL1B) was analyzed by RT-qPCR. The expression of selected cytokines, growth factors and surface molecules was assessed by flow cytometry or ELISA. Generation of reactive oxygen species and induction of DNA breaks were also analyzed. Exposure to diverse NPs caused substantially different molecular responses. No significant effects were detected for NM-100 treatment. NM-200 induced production of IL-8, a potent attractor and activator of neutrophils, growth factors (VEGF and IGF-1) and superoxide. NM-110 triggered a proinflammatory response, characterized by the activation of transcription factor NF-κB, an enhanced production of proinflammatory cytokines (TNF-α) and chemokines (IL-8). Furthermore, the expression of cell adhesion molecules VCAM-1 and ICAM-1 and hepatocyte growth factor (HGF), as well as superoxide production and DNA breaks, were affected. NM-300 K enhanced IL-8 production and induced DNA breaks, however, it decreased the expression of chemokine receptor (CCR2) and CD86 molecule, indicating potential immunosuppressive activity. The toxicity of ZnO and Ag NPs was probably caused by their intracellular dissolution, as indicated by transmission electron microscopy imaging. The observed effects in macrophages might further influence both innate and adaptive immune responses by promoting neutrophil recruitment via IL-8 release and enhancing the adhesion and stimulation of T cells by VCAM-1 and ICAM-1 expression. MDPI 2019-05-02 /pmc/articles/PMC6567235/ /pubmed/31052583 http://dx.doi.org/10.3390/nano9050687 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Brzicova, Tana Javorkova, Eliska Vrbova, Kristyna Zajicova, Alena Holan, Vladimir Pinkas, Dominik Philimonenko, Vlada Sikorova, Jitka Klema, Jiri Topinka, Jan Rossner, Pavel Molecular Responses in THP-1 Macrophage-Like Cells Exposed to Diverse Nanoparticles |
title | Molecular Responses in THP-1 Macrophage-Like Cells Exposed to Diverse Nanoparticles |
title_full | Molecular Responses in THP-1 Macrophage-Like Cells Exposed to Diverse Nanoparticles |
title_fullStr | Molecular Responses in THP-1 Macrophage-Like Cells Exposed to Diverse Nanoparticles |
title_full_unstemmed | Molecular Responses in THP-1 Macrophage-Like Cells Exposed to Diverse Nanoparticles |
title_short | Molecular Responses in THP-1 Macrophage-Like Cells Exposed to Diverse Nanoparticles |
title_sort | molecular responses in thp-1 macrophage-like cells exposed to diverse nanoparticles |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6567235/ https://www.ncbi.nlm.nih.gov/pubmed/31052583 http://dx.doi.org/10.3390/nano9050687 |
work_keys_str_mv | AT brzicovatana molecularresponsesinthp1macrophagelikecellsexposedtodiversenanoparticles AT javorkovaeliska molecularresponsesinthp1macrophagelikecellsexposedtodiversenanoparticles AT vrbovakristyna molecularresponsesinthp1macrophagelikecellsexposedtodiversenanoparticles AT zajicovaalena molecularresponsesinthp1macrophagelikecellsexposedtodiversenanoparticles AT holanvladimir molecularresponsesinthp1macrophagelikecellsexposedtodiversenanoparticles AT pinkasdominik molecularresponsesinthp1macrophagelikecellsexposedtodiversenanoparticles AT philimonenkovlada molecularresponsesinthp1macrophagelikecellsexposedtodiversenanoparticles AT sikorovajitka molecularresponsesinthp1macrophagelikecellsexposedtodiversenanoparticles AT klemajiri molecularresponsesinthp1macrophagelikecellsexposedtodiversenanoparticles AT topinkajan molecularresponsesinthp1macrophagelikecellsexposedtodiversenanoparticles AT rossnerpavel molecularresponsesinthp1macrophagelikecellsexposedtodiversenanoparticles |