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LncRNA GASL1 is downregulated in chronic heart failure and regulates cardiomyocyte apoptosis
BACKGROUND: TGF-β1 contributes to chronic heart failure. It is known that lncRNA GASL1 can inactivate TGF-β1 in cancer biology. METHODS: All the participants were enrolled in the First People’s Hospital of Zhaoqing during the period June 2012 to June 2013. ELISA, RT-qPCR, vectors, transient transfec...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6567419/ https://www.ncbi.nlm.nih.gov/pubmed/31223316 http://dx.doi.org/10.1186/s11658-019-0165-x |
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author | Deng, Haihong Ouyang, Wenbo Zhang, Li Xiao, Xiaoshan Huang, Zhiyong Zhu, Wendian |
author_facet | Deng, Haihong Ouyang, Wenbo Zhang, Li Xiao, Xiaoshan Huang, Zhiyong Zhu, Wendian |
author_sort | Deng, Haihong |
collection | PubMed |
description | BACKGROUND: TGF-β1 contributes to chronic heart failure. It is known that lncRNA GASL1 can inactivate TGF-β1 in cancer biology. METHODS: All the participants were enrolled in the First People’s Hospital of Zhaoqing during the period June 2012 to June 2013. ELISA, RT-qPCR, vectors, transient transfections and western blot were carried out during the research. RESULTS: We found that plasma levels of TGF-β1 were significantly higher, while levels of GASL1 in plasma were significantly lower in chronic heart failure (CHF) patients compared to the control group. TGF-β1 and GASL1 were inversely correlated in CHF patients. Low pretreatment plasma levels of GASL1 were closely associated with poor survival of CHF patients. GASL1 expression was not significantly affected by TGF-β1 overexpression in cardiomyocytes, while cardiomyocytes with GASL1 overexpression showed downregulated TGF-β1. Overexpression of GASL1 led to a decreased, while TGF-β1 overexpression led to an increased apoptotic rate of cardiomyocytes under H(2)O(2) treatment. In addition, TGF-β1 overexpression attenuated the effect of GASL1 overexpression. CONCLUSION: In conclusion, GASL1 was downregulated in CHF. GASL1 overexpression may improve CHF by inhibiting cardiomyocyte apoptosis through the inactivation of TGF-β1. |
format | Online Article Text |
id | pubmed-6567419 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-65674192019-06-20 LncRNA GASL1 is downregulated in chronic heart failure and regulates cardiomyocyte apoptosis Deng, Haihong Ouyang, Wenbo Zhang, Li Xiao, Xiaoshan Huang, Zhiyong Zhu, Wendian Cell Mol Biol Lett Research Letter BACKGROUND: TGF-β1 contributes to chronic heart failure. It is known that lncRNA GASL1 can inactivate TGF-β1 in cancer biology. METHODS: All the participants were enrolled in the First People’s Hospital of Zhaoqing during the period June 2012 to June 2013. ELISA, RT-qPCR, vectors, transient transfections and western blot were carried out during the research. RESULTS: We found that plasma levels of TGF-β1 were significantly higher, while levels of GASL1 in plasma were significantly lower in chronic heart failure (CHF) patients compared to the control group. TGF-β1 and GASL1 were inversely correlated in CHF patients. Low pretreatment plasma levels of GASL1 were closely associated with poor survival of CHF patients. GASL1 expression was not significantly affected by TGF-β1 overexpression in cardiomyocytes, while cardiomyocytes with GASL1 overexpression showed downregulated TGF-β1. Overexpression of GASL1 led to a decreased, while TGF-β1 overexpression led to an increased apoptotic rate of cardiomyocytes under H(2)O(2) treatment. In addition, TGF-β1 overexpression attenuated the effect of GASL1 overexpression. CONCLUSION: In conclusion, GASL1 was downregulated in CHF. GASL1 overexpression may improve CHF by inhibiting cardiomyocyte apoptosis through the inactivation of TGF-β1. BioMed Central 2019-06-13 /pmc/articles/PMC6567419/ /pubmed/31223316 http://dx.doi.org/10.1186/s11658-019-0165-x Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Letter Deng, Haihong Ouyang, Wenbo Zhang, Li Xiao, Xiaoshan Huang, Zhiyong Zhu, Wendian LncRNA GASL1 is downregulated in chronic heart failure and regulates cardiomyocyte apoptosis |
title | LncRNA GASL1 is downregulated in chronic heart failure and regulates cardiomyocyte apoptosis |
title_full | LncRNA GASL1 is downregulated in chronic heart failure and regulates cardiomyocyte apoptosis |
title_fullStr | LncRNA GASL1 is downregulated in chronic heart failure and regulates cardiomyocyte apoptosis |
title_full_unstemmed | LncRNA GASL1 is downregulated in chronic heart failure and regulates cardiomyocyte apoptosis |
title_short | LncRNA GASL1 is downregulated in chronic heart failure and regulates cardiomyocyte apoptosis |
title_sort | lncrna gasl1 is downregulated in chronic heart failure and regulates cardiomyocyte apoptosis |
topic | Research Letter |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6567419/ https://www.ncbi.nlm.nih.gov/pubmed/31223316 http://dx.doi.org/10.1186/s11658-019-0165-x |
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