Cargando…
The phage T4 DNA ligase in vivo improves the survival-coupled bacterial mutagenesis
BACKGROUND: Microbial mutagenesis is an important avenue to acquire microbial strains with desirable traits for industry application. However, mutagens either chemical or physical used often leads narrow library pool due to high lethal rate. The T4 DNA ligase is one of the most widely utilized enzym...
Autores principales: | Wang, Junshu, Liu, Fapeng, Su, Tianyuan, Chang, Yizhao, Guo, Qi, Wang, Qian, Liang, Quanfeng, Qi, Qingsheng |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6567493/ https://www.ncbi.nlm.nih.gov/pubmed/31196093 http://dx.doi.org/10.1186/s12934-019-1160-7 |
Ejemplares similares
-
The phage T4 DNA ligase mediates bacterial chromosome DSBs repair as single component non-homologous end joining
por: Su, Tianyuan, et al.
Publicado: (2019) -
A CRISPR-Cas9 Assisted Non-Homologous End-Joining Strategy for One-step Engineering of Bacterial Genome
por: Su, Tianyuan, et al.
Publicado: (2016) -
Easy regulation of metabolic flux in Escherichia coli using an endogenous type I-E CRISPR-Cas system
por: Chang, Yizhao, et al.
Publicado: (2016) -
Base editing-coupled survival screening enabled high-sensitive analysis of PAM compatibility and finding of the new possible off-target
por: Su, Tianyuan, et al.
Publicado: (2021) -
Random genome reduction coupled with polyhydroxybutyrate biosynthesis to facilitate its accumulation in Escherichia coli
por: Ma, Shuai, et al.
Publicado: (2022)