Cargando…
let-7i inhibits proliferation and migration of bladder cancer cells by targeting HMGA1
BACKGROUND: Let-7 is one of the earliest discovered microRNAs(miRNAs) and has been reported to be down-regulated in multiple malignant tumors. The effects and molecular mechanisms of let-7i in bladder cancer are still unclear. This study was to investigate the effects and potential mechanisms of let...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6567622/ https://www.ncbi.nlm.nih.gov/pubmed/31196036 http://dx.doi.org/10.1186/s12894-019-0485-1 |
_version_ | 1783427118263173120 |
---|---|
author | Qin, M-M Chai, X. Huang, H-B Feng, G. Li, X-N Zhang, J. Zheng, R. Liu, X-C Pu, C. |
author_facet | Qin, M-M Chai, X. Huang, H-B Feng, G. Li, X-N Zhang, J. Zheng, R. Liu, X-C Pu, C. |
author_sort | Qin, M-M |
collection | PubMed |
description | BACKGROUND: Let-7 is one of the earliest discovered microRNAs(miRNAs) and has been reported to be down-regulated in multiple malignant tumors. The effects and molecular mechanisms of let-7i in bladder cancer are still unclear. This study was to investigate the effects and potential mechanisms of let-7i on bladder cancer cells. METHODS: Total RNA was extracted from bladder cancer cell lines. The expression levels of let-7i and HMGA1 were examined by quantitative real-time PCR. Cell viability was detected using the CCK-8 and colony formation assays, while transwell and wound healing assays were used to evaluate migration ability. Luciferase reporter assay and western blot were used to confirm the target gene of let-7i. RESULTS: Compared with the SV-40 immortalized human uroepithelial cell line (SV-HUC-1), bladder cancer cell lines T24 and 5637 had low levels of let-7i expression, but high levels of high mobility group protein A1 (HMGA1) expression. Transfection of cell lines T24 and 5637 with let-7i mimic suppressed cell proliferation and migration. Luciferase reporter assay confirmed HMGA1 may be one of the target genes of let-7i-5p. Protein and mRNA expression of HMGA1 was significantly downregulated in let-7i mimic transfected cell lines T24 and 5637. CONCLUSIONS: Up-regulation of let-7i suppressed proliferation and migration of the human bladder cancer cell lines T24 and 5637 by targeting HMGA1. These findings suggest that let-7i might be considered as a novel therapeutic target for bladder cancer. |
format | Online Article Text |
id | pubmed-6567622 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-65676222019-06-27 let-7i inhibits proliferation and migration of bladder cancer cells by targeting HMGA1 Qin, M-M Chai, X. Huang, H-B Feng, G. Li, X-N Zhang, J. Zheng, R. Liu, X-C Pu, C. BMC Urol Research Article BACKGROUND: Let-7 is one of the earliest discovered microRNAs(miRNAs) and has been reported to be down-regulated in multiple malignant tumors. The effects and molecular mechanisms of let-7i in bladder cancer are still unclear. This study was to investigate the effects and potential mechanisms of let-7i on bladder cancer cells. METHODS: Total RNA was extracted from bladder cancer cell lines. The expression levels of let-7i and HMGA1 were examined by quantitative real-time PCR. Cell viability was detected using the CCK-8 and colony formation assays, while transwell and wound healing assays were used to evaluate migration ability. Luciferase reporter assay and western blot were used to confirm the target gene of let-7i. RESULTS: Compared with the SV-40 immortalized human uroepithelial cell line (SV-HUC-1), bladder cancer cell lines T24 and 5637 had low levels of let-7i expression, but high levels of high mobility group protein A1 (HMGA1) expression. Transfection of cell lines T24 and 5637 with let-7i mimic suppressed cell proliferation and migration. Luciferase reporter assay confirmed HMGA1 may be one of the target genes of let-7i-5p. Protein and mRNA expression of HMGA1 was significantly downregulated in let-7i mimic transfected cell lines T24 and 5637. CONCLUSIONS: Up-regulation of let-7i suppressed proliferation and migration of the human bladder cancer cell lines T24 and 5637 by targeting HMGA1. These findings suggest that let-7i might be considered as a novel therapeutic target for bladder cancer. BioMed Central 2019-06-13 /pmc/articles/PMC6567622/ /pubmed/31196036 http://dx.doi.org/10.1186/s12894-019-0485-1 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Qin, M-M Chai, X. Huang, H-B Feng, G. Li, X-N Zhang, J. Zheng, R. Liu, X-C Pu, C. let-7i inhibits proliferation and migration of bladder cancer cells by targeting HMGA1 |
title | let-7i inhibits proliferation and migration of bladder cancer cells by targeting HMGA1 |
title_full | let-7i inhibits proliferation and migration of bladder cancer cells by targeting HMGA1 |
title_fullStr | let-7i inhibits proliferation and migration of bladder cancer cells by targeting HMGA1 |
title_full_unstemmed | let-7i inhibits proliferation and migration of bladder cancer cells by targeting HMGA1 |
title_short | let-7i inhibits proliferation and migration of bladder cancer cells by targeting HMGA1 |
title_sort | let-7i inhibits proliferation and migration of bladder cancer cells by targeting hmga1 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6567622/ https://www.ncbi.nlm.nih.gov/pubmed/31196036 http://dx.doi.org/10.1186/s12894-019-0485-1 |
work_keys_str_mv | AT qinmm let7iinhibitsproliferationandmigrationofbladdercancercellsbytargetinghmga1 AT chaix let7iinhibitsproliferationandmigrationofbladdercancercellsbytargetinghmga1 AT huanghb let7iinhibitsproliferationandmigrationofbladdercancercellsbytargetinghmga1 AT fengg let7iinhibitsproliferationandmigrationofbladdercancercellsbytargetinghmga1 AT lixn let7iinhibitsproliferationandmigrationofbladdercancercellsbytargetinghmga1 AT zhangj let7iinhibitsproliferationandmigrationofbladdercancercellsbytargetinghmga1 AT zhengr let7iinhibitsproliferationandmigrationofbladdercancercellsbytargetinghmga1 AT liuxc let7iinhibitsproliferationandmigrationofbladdercancercellsbytargetinghmga1 AT puc let7iinhibitsproliferationandmigrationofbladdercancercellsbytargetinghmga1 |