Cargando…
Modified Chuanhu anti-gout mixture, a traditional Chinese medicine, protects against potassium oxonate-induced hyperuricemia and renal dysfunction in mice
OBJECTIVE: Acute gout is a painful, inflammatory arthritis that features a rapidly escalating inflammatory response resulting from the formation of monosodium urate crystals in the affected joint space. Previously, we found that Chuanhu anti-gout mixture (CAGM) had similar effects as colchicine agai...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6567774/ https://www.ncbi.nlm.nih.gov/pubmed/30832523 http://dx.doi.org/10.1177/0300060519831182 |
_version_ | 1783427153386274816 |
---|---|
author | You, Wenjun Wang, Jie Zou, Yaowu Che, Kui Hou, Xu Fei, Honghua Wang, Yangang |
author_facet | You, Wenjun Wang, Jie Zou, Yaowu Che, Kui Hou, Xu Fei, Honghua Wang, Yangang |
author_sort | You, Wenjun |
collection | PubMed |
description | OBJECTIVE: Acute gout is a painful, inflammatory arthritis that features a rapidly escalating inflammatory response resulting from the formation of monosodium urate crystals in the affected joint space. Previously, we found that Chuanhu anti-gout mixture (CAGM) had similar effects as colchicine against gout in the clinic. Subsequently, to improve its effectiveness and efficacy, we modified the original formulation of CAGM. The current study evaluated the effectiveness of the modified formulation in mice. METHODS: Potassium oxonate (PO) was used to establish a mouse model of hyperuricemia. Plasma levels of uric acid and creatine were determined using the respective test kits. Hepatic xanthine oxidase (XOD) expression was examined by enzyme-linked immunosorbent assay. To explore the underlying mechanism, renal urate transporter 1 (URAT1) mRNA levels were evaluated by quantitative real-time PCR. Allopurinol and benzbromarone were used as reference drugs. RESULTS: The original CAGM and its modified high-dose formulation significantly reduced serum uric acid and creatine levels in hyperuricemic mice. In addition, the CAGM-treated groups displayed lower mRNA levels of hepatic XOD and renal URAT1. CONCLUSIONS: CAGM and its modified formulation significantly ameliorated PO-induced hyperuricemia in mice, which might be partially attributable to reductions of hepatic XOD and renal URAT1 levels. |
format | Online Article Text |
id | pubmed-6567774 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-65677742019-06-20 Modified Chuanhu anti-gout mixture, a traditional Chinese medicine, protects against potassium oxonate-induced hyperuricemia and renal dysfunction in mice You, Wenjun Wang, Jie Zou, Yaowu Che, Kui Hou, Xu Fei, Honghua Wang, Yangang J Int Med Res Clinical Research Reports OBJECTIVE: Acute gout is a painful, inflammatory arthritis that features a rapidly escalating inflammatory response resulting from the formation of monosodium urate crystals in the affected joint space. Previously, we found that Chuanhu anti-gout mixture (CAGM) had similar effects as colchicine against gout in the clinic. Subsequently, to improve its effectiveness and efficacy, we modified the original formulation of CAGM. The current study evaluated the effectiveness of the modified formulation in mice. METHODS: Potassium oxonate (PO) was used to establish a mouse model of hyperuricemia. Plasma levels of uric acid and creatine were determined using the respective test kits. Hepatic xanthine oxidase (XOD) expression was examined by enzyme-linked immunosorbent assay. To explore the underlying mechanism, renal urate transporter 1 (URAT1) mRNA levels were evaluated by quantitative real-time PCR. Allopurinol and benzbromarone were used as reference drugs. RESULTS: The original CAGM and its modified high-dose formulation significantly reduced serum uric acid and creatine levels in hyperuricemic mice. In addition, the CAGM-treated groups displayed lower mRNA levels of hepatic XOD and renal URAT1. CONCLUSIONS: CAGM and its modified formulation significantly ameliorated PO-induced hyperuricemia in mice, which might be partially attributable to reductions of hepatic XOD and renal URAT1 levels. SAGE Publications 2019-03-05 2019-05 /pmc/articles/PMC6567774/ /pubmed/30832523 http://dx.doi.org/10.1177/0300060519831182 Text en © The Author(s) 2019 http://creativecommons.org/licenses/by-nc/4.0/ Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Clinical Research Reports You, Wenjun Wang, Jie Zou, Yaowu Che, Kui Hou, Xu Fei, Honghua Wang, Yangang Modified Chuanhu anti-gout mixture, a traditional Chinese medicine, protects against potassium oxonate-induced hyperuricemia and renal dysfunction in mice |
title | Modified Chuanhu anti-gout mixture, a traditional Chinese medicine, protects against potassium oxonate-induced hyperuricemia and renal dysfunction in mice |
title_full | Modified Chuanhu anti-gout mixture, a traditional Chinese medicine, protects against potassium oxonate-induced hyperuricemia and renal dysfunction in mice |
title_fullStr | Modified Chuanhu anti-gout mixture, a traditional Chinese medicine, protects against potassium oxonate-induced hyperuricemia and renal dysfunction in mice |
title_full_unstemmed | Modified Chuanhu anti-gout mixture, a traditional Chinese medicine, protects against potassium oxonate-induced hyperuricemia and renal dysfunction in mice |
title_short | Modified Chuanhu anti-gout mixture, a traditional Chinese medicine, protects against potassium oxonate-induced hyperuricemia and renal dysfunction in mice |
title_sort | modified chuanhu anti-gout mixture, a traditional chinese medicine, protects against potassium oxonate-induced hyperuricemia and renal dysfunction in mice |
topic | Clinical Research Reports |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6567774/ https://www.ncbi.nlm.nih.gov/pubmed/30832523 http://dx.doi.org/10.1177/0300060519831182 |
work_keys_str_mv | AT youwenjun modifiedchuanhuantigoutmixtureatraditionalchinesemedicineprotectsagainstpotassiumoxonateinducedhyperuricemiaandrenaldysfunctioninmice AT wangjie modifiedchuanhuantigoutmixtureatraditionalchinesemedicineprotectsagainstpotassiumoxonateinducedhyperuricemiaandrenaldysfunctioninmice AT zouyaowu modifiedchuanhuantigoutmixtureatraditionalchinesemedicineprotectsagainstpotassiumoxonateinducedhyperuricemiaandrenaldysfunctioninmice AT chekui modifiedchuanhuantigoutmixtureatraditionalchinesemedicineprotectsagainstpotassiumoxonateinducedhyperuricemiaandrenaldysfunctioninmice AT houxu modifiedchuanhuantigoutmixtureatraditionalchinesemedicineprotectsagainstpotassiumoxonateinducedhyperuricemiaandrenaldysfunctioninmice AT feihonghua modifiedchuanhuantigoutmixtureatraditionalchinesemedicineprotectsagainstpotassiumoxonateinducedhyperuricemiaandrenaldysfunctioninmice AT wangyangang modifiedchuanhuantigoutmixtureatraditionalchinesemedicineprotectsagainstpotassiumoxonateinducedhyperuricemiaandrenaldysfunctioninmice |