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Dam mutants provide improved sensitivity and spatial resolution for profiling transcription factor binding

DamID, in which a protein of interest is fused to Dam methylase, enables mapping of protein-DNA binding through readout of adenine methylation in genomic DNA. DamID offers a compelling alternative to chromatin immunoprecipitation sequencing (ChIP-Seq), particularly in cases where cell number or anti...

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Detalles Bibliográficos
Autores principales:	Szczesnik, Tomasz, Ho, Joshua W. K., Sherwood, Richard
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2019
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6567924/ https://www.ncbi.nlm.nih.gov/pubmed/31196130 http://dx.doi.org/10.1186/s13072-019-0273-x

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