Exploration of Performance Kinetics and Mechanism of Action of a Potential Novel Bioflocculant BF-VB2 on Clay and Dye Wastewater Flocculation

This study explores production of an efficient bioflocculant; BF-VB2, by strain Bacillus sp. TERI VB2 and proposes its potential application in wastewater treatment. One milligram of BF-VB2 can effectively flocculate 1980.0 mg ± 5.0 mg of kaolin particles leading to 99.0% ± 0.5% enhancement in flocculation activity and 99.6% ± 1.0% reduction in turbidity; in less time. BF-VB2 when applied for treatment of textile dyeing industrial wastewater revealed reduction in dye color (82.78% ± 3.03%), COD (92.54% ± 0.24%), TSS (73.59% ± 0.71%), and chloride ions (81.90% ± 0.716%). The best-fit kinetic model (for both COD removal, and dye decolorization) was pseudo-first order with regression coefficient of 0.98 and 0.95, and rate constant of 4.33 × 10(-2) and 1.83 × 10(2), respectively. Bridging due to presence of surface charges have been proposed as flocculation mechanism. From results obtained during test-tube studies, flocculation in larger volumes (0.01–5.0 L) was also performed to intend taking up BF-VB2 for in situ industrial wastewater treatment. This eco-friendly polysaccharide bioflocculant had longer shelf-life, stability to pH and temperature, cation-independence, and emerged to be more efficient than other flocculants assessed. This study proposed BF-VB2 as a potential natural flocculant candidate for industrial application.