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Breast Cancer Subtypes Present a Differential Production of Reactive Oxygen Species (ROS) and Susceptibility to Antioxidant Treatment

Due to their crucial role in cell metabolism and homeostasis, alterations in mitochondrial biology and function have been related to the progression of diverse diseases including cancer. One of the consequences associated to mitochondrial dysfunction is the production of reactive oxygen species (ROS...

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Autores principales: Sarmiento-Salinas, Fabiola Lilí, Delgado-Magallón, Alam, Montes-Alvarado, José Benito, Ramírez-Ramírez, Dalia, Flores-Alonso, Juan Carlos, Cortés-Hernández, Paulina, Reyes-Leyva, Julio, Herrera-Camacho, Irma, Anaya-Ruiz, Maricruz, Pelayo, Rosana, Millán-Pérez-Peña, Lourdes, Maycotte, Paola
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6568240/
https://www.ncbi.nlm.nih.gov/pubmed/31231612
http://dx.doi.org/10.3389/fonc.2019.00480
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author Sarmiento-Salinas, Fabiola Lilí
Delgado-Magallón, Alam
Montes-Alvarado, José Benito
Ramírez-Ramírez, Dalia
Flores-Alonso, Juan Carlos
Cortés-Hernández, Paulina
Reyes-Leyva, Julio
Herrera-Camacho, Irma
Anaya-Ruiz, Maricruz
Pelayo, Rosana
Millán-Pérez-Peña, Lourdes
Maycotte, Paola
author_facet Sarmiento-Salinas, Fabiola Lilí
Delgado-Magallón, Alam
Montes-Alvarado, José Benito
Ramírez-Ramírez, Dalia
Flores-Alonso, Juan Carlos
Cortés-Hernández, Paulina
Reyes-Leyva, Julio
Herrera-Camacho, Irma
Anaya-Ruiz, Maricruz
Pelayo, Rosana
Millán-Pérez-Peña, Lourdes
Maycotte, Paola
author_sort Sarmiento-Salinas, Fabiola Lilí
collection PubMed
description Due to their crucial role in cell metabolism and homeostasis, alterations in mitochondrial biology and function have been related to the progression of diverse diseases including cancer. One of the consequences associated to mitochondrial dysfunction is the production of reactive oxygen species (ROS). ROS are known to have a controversial role during cancer initiation and progression and although several studies have tried to manipulate intracellular ROS levels using antioxidants or pro-oxidation conditions, it is not yet clear how to target oxidation for cancer therapy. In this study, we found differences in mitochondrial morphology in breast cancer cells when compared to a non-tumorigenic cell line and differences in mitochondrial function among breast cancer subtypes when exploring gene-expression data from the TCGA tumor dataset. Interestingly, we found increased ROS levels in triple negative breast cancer (TNBC) cell lines and a dependency on ROS for survival since antioxidant treatment induced cell death in TNBC cells but not in an estrogen receptor positive (ER+) cell line. Moreover, we identified the mitochondria as the main source of ROS in TNBC cell lines. Our results indicate a potential use for ROS as a target for therapy in the TNBC subtype which currently has the worst prognosis among all breast cancers and remains as the only breast cancer subtype which lacks a targeted therapy.
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spelling pubmed-65682402019-06-21 Breast Cancer Subtypes Present a Differential Production of Reactive Oxygen Species (ROS) and Susceptibility to Antioxidant Treatment Sarmiento-Salinas, Fabiola Lilí Delgado-Magallón, Alam Montes-Alvarado, José Benito Ramírez-Ramírez, Dalia Flores-Alonso, Juan Carlos Cortés-Hernández, Paulina Reyes-Leyva, Julio Herrera-Camacho, Irma Anaya-Ruiz, Maricruz Pelayo, Rosana Millán-Pérez-Peña, Lourdes Maycotte, Paola Front Oncol Oncology Due to their crucial role in cell metabolism and homeostasis, alterations in mitochondrial biology and function have been related to the progression of diverse diseases including cancer. One of the consequences associated to mitochondrial dysfunction is the production of reactive oxygen species (ROS). ROS are known to have a controversial role during cancer initiation and progression and although several studies have tried to manipulate intracellular ROS levels using antioxidants or pro-oxidation conditions, it is not yet clear how to target oxidation for cancer therapy. In this study, we found differences in mitochondrial morphology in breast cancer cells when compared to a non-tumorigenic cell line and differences in mitochondrial function among breast cancer subtypes when exploring gene-expression data from the TCGA tumor dataset. Interestingly, we found increased ROS levels in triple negative breast cancer (TNBC) cell lines and a dependency on ROS for survival since antioxidant treatment induced cell death in TNBC cells but not in an estrogen receptor positive (ER+) cell line. Moreover, we identified the mitochondria as the main source of ROS in TNBC cell lines. Our results indicate a potential use for ROS as a target for therapy in the TNBC subtype which currently has the worst prognosis among all breast cancers and remains as the only breast cancer subtype which lacks a targeted therapy. Frontiers Media S.A. 2019-06-07 /pmc/articles/PMC6568240/ /pubmed/31231612 http://dx.doi.org/10.3389/fonc.2019.00480 Text en Copyright © 2019 Sarmiento-Salinas, Delgado-Magallón, Montes-Alvarado, Ramírez-Ramírez, Flores-Alonso, Cortés-Hernández, Reyes-Leyva, Herrera-Camacho, Anaya-Ruiz, Pelayo, Millán-Pérez-Peña and Maycotte. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Sarmiento-Salinas, Fabiola Lilí
Delgado-Magallón, Alam
Montes-Alvarado, José Benito
Ramírez-Ramírez, Dalia
Flores-Alonso, Juan Carlos
Cortés-Hernández, Paulina
Reyes-Leyva, Julio
Herrera-Camacho, Irma
Anaya-Ruiz, Maricruz
Pelayo, Rosana
Millán-Pérez-Peña, Lourdes
Maycotte, Paola
Breast Cancer Subtypes Present a Differential Production of Reactive Oxygen Species (ROS) and Susceptibility to Antioxidant Treatment
title Breast Cancer Subtypes Present a Differential Production of Reactive Oxygen Species (ROS) and Susceptibility to Antioxidant Treatment
title_full Breast Cancer Subtypes Present a Differential Production of Reactive Oxygen Species (ROS) and Susceptibility to Antioxidant Treatment
title_fullStr Breast Cancer Subtypes Present a Differential Production of Reactive Oxygen Species (ROS) and Susceptibility to Antioxidant Treatment
title_full_unstemmed Breast Cancer Subtypes Present a Differential Production of Reactive Oxygen Species (ROS) and Susceptibility to Antioxidant Treatment
title_short Breast Cancer Subtypes Present a Differential Production of Reactive Oxygen Species (ROS) and Susceptibility to Antioxidant Treatment
title_sort breast cancer subtypes present a differential production of reactive oxygen species (ros) and susceptibility to antioxidant treatment
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6568240/
https://www.ncbi.nlm.nih.gov/pubmed/31231612
http://dx.doi.org/10.3389/fonc.2019.00480
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