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The role of acetyl-coA carboxylase2 in head and neck squamous cell carcinoma
BACKGROUND: Acetyl-CoA carboxylase (ACC) plays an important role in the metabolism of various cancer cells, but its role in head and neck squamous cell carcinoma (HNSCC) is uncertain. Therefore, in the present study, we explored the role of ACC2 in HNSCC. METHODS: Western blot and immunohistochemist...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6568254/ https://www.ncbi.nlm.nih.gov/pubmed/31218122 http://dx.doi.org/10.7717/peerj.7037 |
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author | Li, Kun Zhang, Chengcheng Chen, Lei Wang, Pingping Fang, Yang Zhu, Junwei Chen, Shuo Du, Juan Shen, Bing Wu, Kaile Liu, Yehai |
author_facet | Li, Kun Zhang, Chengcheng Chen, Lei Wang, Pingping Fang, Yang Zhu, Junwei Chen, Shuo Du, Juan Shen, Bing Wu, Kaile Liu, Yehai |
author_sort | Li, Kun |
collection | PubMed |
description | BACKGROUND: Acetyl-CoA carboxylase (ACC) plays an important role in the metabolism of various cancer cells, but its role in head and neck squamous cell carcinoma (HNSCC) is uncertain. Therefore, in the present study, we explored the role of ACC2 in HNSCC. METHODS: Western blot and immunohistochemistry assays were used to determine ACC2 protein expression levels in laryngocarcinoma and adjacent normal tissues derived from patients with laryngocarcinoma. ACC2 expression was knocked down in the hypopharyngeal cancer cell line FaDu to determine its effect on apoptosis. Lipid oil red staining was used to test the change of intracellular lipid. RESULTS: The results showed that the ACC2 protein was highly expressed in laryngocarcinoma and that the ACC2 expression level was positively associated with the clinical cancer stage and negatively associated with the degree of laryngocarcinoma cell differentiation. Kaplan–Meier analyses indicated that compared with patients having low levels of ACC2, those with high ACC2 levels had a decreased 5-year survival rate. The results of western blot and terminal deoxynucleotidyl transferase dUTP nick-end labeling assays showed that knockdown of ACC2 accelerated apoptosis in FaDu cells. Furthermore, knockdown of ACC2 significantly reduced the intracellular lipid levels in FaDu cells. CONCLUSION: These findings suggest that ACC2 may be an important prognostic marker for patients with HNSCC and that ACC2 may be a potential target in the treatment of HNSCC. |
format | Online Article Text |
id | pubmed-6568254 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-65682542019-06-19 The role of acetyl-coA carboxylase2 in head and neck squamous cell carcinoma Li, Kun Zhang, Chengcheng Chen, Lei Wang, Pingping Fang, Yang Zhu, Junwei Chen, Shuo Du, Juan Shen, Bing Wu, Kaile Liu, Yehai PeerJ Biochemistry BACKGROUND: Acetyl-CoA carboxylase (ACC) plays an important role in the metabolism of various cancer cells, but its role in head and neck squamous cell carcinoma (HNSCC) is uncertain. Therefore, in the present study, we explored the role of ACC2 in HNSCC. METHODS: Western blot and immunohistochemistry assays were used to determine ACC2 protein expression levels in laryngocarcinoma and adjacent normal tissues derived from patients with laryngocarcinoma. ACC2 expression was knocked down in the hypopharyngeal cancer cell line FaDu to determine its effect on apoptosis. Lipid oil red staining was used to test the change of intracellular lipid. RESULTS: The results showed that the ACC2 protein was highly expressed in laryngocarcinoma and that the ACC2 expression level was positively associated with the clinical cancer stage and negatively associated with the degree of laryngocarcinoma cell differentiation. Kaplan–Meier analyses indicated that compared with patients having low levels of ACC2, those with high ACC2 levels had a decreased 5-year survival rate. The results of western blot and terminal deoxynucleotidyl transferase dUTP nick-end labeling assays showed that knockdown of ACC2 accelerated apoptosis in FaDu cells. Furthermore, knockdown of ACC2 significantly reduced the intracellular lipid levels in FaDu cells. CONCLUSION: These findings suggest that ACC2 may be an important prognostic marker for patients with HNSCC and that ACC2 may be a potential target in the treatment of HNSCC. PeerJ Inc. 2019-06-11 /pmc/articles/PMC6568254/ /pubmed/31218122 http://dx.doi.org/10.7717/peerj.7037 Text en © 2019 Li et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Biochemistry Li, Kun Zhang, Chengcheng Chen, Lei Wang, Pingping Fang, Yang Zhu, Junwei Chen, Shuo Du, Juan Shen, Bing Wu, Kaile Liu, Yehai The role of acetyl-coA carboxylase2 in head and neck squamous cell carcinoma |
title | The role of acetyl-coA carboxylase2 in head and neck squamous cell carcinoma |
title_full | The role of acetyl-coA carboxylase2 in head and neck squamous cell carcinoma |
title_fullStr | The role of acetyl-coA carboxylase2 in head and neck squamous cell carcinoma |
title_full_unstemmed | The role of acetyl-coA carboxylase2 in head and neck squamous cell carcinoma |
title_short | The role of acetyl-coA carboxylase2 in head and neck squamous cell carcinoma |
title_sort | role of acetyl-coa carboxylase2 in head and neck squamous cell carcinoma |
topic | Biochemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6568254/ https://www.ncbi.nlm.nih.gov/pubmed/31218122 http://dx.doi.org/10.7717/peerj.7037 |
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