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Genomic signatures of heterokaryosis in the oomycete pathogen Bremia lactucae

Lettuce downy mildew caused by Bremia lactucae is the most important disease of lettuce globally. This oomycete is highly variable and rapidly overcomes resistance genes and fungicides. The use of multiple read types results in a high-quality, near-chromosome-scale, consensus assembly. Flow cytometr...

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Autores principales: Fletcher, Kyle, Gil, Juliana, Bertier, Lien D., Kenefick, Aubrey, Wood, Kelsey J., Zhang, Lin, Reyes-Chin-Wo, Sebastian, Cavanaugh, Keri, Tsuchida, Cayla, Wong, Joan, Michelmore, Richard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6570648/
https://www.ncbi.nlm.nih.gov/pubmed/31201315
http://dx.doi.org/10.1038/s41467-019-10550-0
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author Fletcher, Kyle
Gil, Juliana
Bertier, Lien D.
Kenefick, Aubrey
Wood, Kelsey J.
Zhang, Lin
Reyes-Chin-Wo, Sebastian
Cavanaugh, Keri
Tsuchida, Cayla
Wong, Joan
Michelmore, Richard
author_facet Fletcher, Kyle
Gil, Juliana
Bertier, Lien D.
Kenefick, Aubrey
Wood, Kelsey J.
Zhang, Lin
Reyes-Chin-Wo, Sebastian
Cavanaugh, Keri
Tsuchida, Cayla
Wong, Joan
Michelmore, Richard
author_sort Fletcher, Kyle
collection PubMed
description Lettuce downy mildew caused by Bremia lactucae is the most important disease of lettuce globally. This oomycete is highly variable and rapidly overcomes resistance genes and fungicides. The use of multiple read types results in a high-quality, near-chromosome-scale, consensus assembly. Flow cytometry plus resequencing of 30 field isolates, 37 sexual offspring, and 19 asexual derivatives from single multinucleate sporangia demonstrates a high incidence of heterokaryosis in B. lactucae. Heterokaryosis has phenotypic consequences on fitness that may include an increased sporulation rate and qualitative differences in virulence. Therefore, selection should be considered as acting on a population of nuclei within coenocytic mycelia. This provides evolutionary flexibility to the pathogen enabling rapid adaptation to different repertoires of host resistance genes and other challenges. The advantages of asexual persistence of heterokaryons may have been one of the drivers of selection that resulted in the loss of uninucleate zoospores in multiple downy mildews.
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spelling pubmed-65706482019-06-24 Genomic signatures of heterokaryosis in the oomycete pathogen Bremia lactucae Fletcher, Kyle Gil, Juliana Bertier, Lien D. Kenefick, Aubrey Wood, Kelsey J. Zhang, Lin Reyes-Chin-Wo, Sebastian Cavanaugh, Keri Tsuchida, Cayla Wong, Joan Michelmore, Richard Nat Commun Article Lettuce downy mildew caused by Bremia lactucae is the most important disease of lettuce globally. This oomycete is highly variable and rapidly overcomes resistance genes and fungicides. The use of multiple read types results in a high-quality, near-chromosome-scale, consensus assembly. Flow cytometry plus resequencing of 30 field isolates, 37 sexual offspring, and 19 asexual derivatives from single multinucleate sporangia demonstrates a high incidence of heterokaryosis in B. lactucae. Heterokaryosis has phenotypic consequences on fitness that may include an increased sporulation rate and qualitative differences in virulence. Therefore, selection should be considered as acting on a population of nuclei within coenocytic mycelia. This provides evolutionary flexibility to the pathogen enabling rapid adaptation to different repertoires of host resistance genes and other challenges. The advantages of asexual persistence of heterokaryons may have been one of the drivers of selection that resulted in the loss of uninucleate zoospores in multiple downy mildews. Nature Publishing Group UK 2019-06-14 /pmc/articles/PMC6570648/ /pubmed/31201315 http://dx.doi.org/10.1038/s41467-019-10550-0 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Fletcher, Kyle
Gil, Juliana
Bertier, Lien D.
Kenefick, Aubrey
Wood, Kelsey J.
Zhang, Lin
Reyes-Chin-Wo, Sebastian
Cavanaugh, Keri
Tsuchida, Cayla
Wong, Joan
Michelmore, Richard
Genomic signatures of heterokaryosis in the oomycete pathogen Bremia lactucae
title Genomic signatures of heterokaryosis in the oomycete pathogen Bremia lactucae
title_full Genomic signatures of heterokaryosis in the oomycete pathogen Bremia lactucae
title_fullStr Genomic signatures of heterokaryosis in the oomycete pathogen Bremia lactucae
title_full_unstemmed Genomic signatures of heterokaryosis in the oomycete pathogen Bremia lactucae
title_short Genomic signatures of heterokaryosis in the oomycete pathogen Bremia lactucae
title_sort genomic signatures of heterokaryosis in the oomycete pathogen bremia lactucae
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6570648/
https://www.ncbi.nlm.nih.gov/pubmed/31201315
http://dx.doi.org/10.1038/s41467-019-10550-0
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