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Molecular evidence of Tula virus in Microtus obscurus in the region of Yili, Xinjiang, China
BACKGROUND: Hantaviruses are important zoonotic pathogens, and they pose a profound risk to public health. So far, there has been no evidence showing that Tula virus (TULV), one species of hantavirus, is endemic in China. In this study, we captured rodents and found that the Tula virus had infected...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6570900/ https://www.ncbi.nlm.nih.gov/pubmed/31200661 http://dx.doi.org/10.1186/s12879-019-4133-x |
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author | Guo, Gang Guo, Baoping Wu, Xiran Wang, Yuanzhi Bao, Jianling Ren, Yuan Li, Hongyu Li, Jun Zhang, Wenbao Yao, Hua |
author_facet | Guo, Gang Guo, Baoping Wu, Xiran Wang, Yuanzhi Bao, Jianling Ren, Yuan Li, Hongyu Li, Jun Zhang, Wenbao Yao, Hua |
author_sort | Guo, Gang |
collection | PubMed |
description | BACKGROUND: Hantaviruses are important zoonotic pathogens, and they pose a profound risk to public health. So far, there has been no evidence showing that Tula virus (TULV), one species of hantavirus, is endemic in China. In this study, we captured rodents and found that the Tula virus had infected voles in Yili region, Xinjiang, China. METHODS: Rodents were captured by flooding their burrows in mountain pasture areas in Narati, Xinyuan County, Xinjiang, China. Hantavirus L gene fragments were amplified by nest RT-PCR using genus-specific primers. Positive samples were further identified by sequencing of RT-PCR products of S gene fragment for species identification. To identify the species of captured small mammals, the rodents’ cytochrome b (Cytb) was amplified by PCR and sequenced. Phylogenetic analysis was used to show the clustering and evolution relationship of the viral nucleic acids. RESULTS: Here, 31 out of 198 voles captured (16%) were infected with TULV. Host sequencing analysis showed these voles were Microtus obscurus (M. obscurs). Alignment and phylogenetic analysis of the exon region (1191 bp) of the hantavirus S gene confirmed that all of the detected amplicons were TULV, which was similar to one strain of TULV identified in Kazakhstan. CONCLUSION: This is the first identification of Tula virus in China, and we found that M. obscurus acts as a natural reservoir for carrying the virus. Although the infection rate in the local human population remains unknown, the high prevalence of TULV in the small mammals in the region constitutes a risk that this putative pathogen may spread to the local population. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12879-019-4133-x) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6570900 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-65709002019-06-27 Molecular evidence of Tula virus in Microtus obscurus in the region of Yili, Xinjiang, China Guo, Gang Guo, Baoping Wu, Xiran Wang, Yuanzhi Bao, Jianling Ren, Yuan Li, Hongyu Li, Jun Zhang, Wenbao Yao, Hua BMC Infect Dis Research Article BACKGROUND: Hantaviruses are important zoonotic pathogens, and they pose a profound risk to public health. So far, there has been no evidence showing that Tula virus (TULV), one species of hantavirus, is endemic in China. In this study, we captured rodents and found that the Tula virus had infected voles in Yili region, Xinjiang, China. METHODS: Rodents were captured by flooding their burrows in mountain pasture areas in Narati, Xinyuan County, Xinjiang, China. Hantavirus L gene fragments were amplified by nest RT-PCR using genus-specific primers. Positive samples were further identified by sequencing of RT-PCR products of S gene fragment for species identification. To identify the species of captured small mammals, the rodents’ cytochrome b (Cytb) was amplified by PCR and sequenced. Phylogenetic analysis was used to show the clustering and evolution relationship of the viral nucleic acids. RESULTS: Here, 31 out of 198 voles captured (16%) were infected with TULV. Host sequencing analysis showed these voles were Microtus obscurus (M. obscurs). Alignment and phylogenetic analysis of the exon region (1191 bp) of the hantavirus S gene confirmed that all of the detected amplicons were TULV, which was similar to one strain of TULV identified in Kazakhstan. CONCLUSION: This is the first identification of Tula virus in China, and we found that M. obscurus acts as a natural reservoir for carrying the virus. Although the infection rate in the local human population remains unknown, the high prevalence of TULV in the small mammals in the region constitutes a risk that this putative pathogen may spread to the local population. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12879-019-4133-x) contains supplementary material, which is available to authorized users. BioMed Central 2019-06-14 /pmc/articles/PMC6570900/ /pubmed/31200661 http://dx.doi.org/10.1186/s12879-019-4133-x Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Guo, Gang Guo, Baoping Wu, Xiran Wang, Yuanzhi Bao, Jianling Ren, Yuan Li, Hongyu Li, Jun Zhang, Wenbao Yao, Hua Molecular evidence of Tula virus in Microtus obscurus in the region of Yili, Xinjiang, China |
title | Molecular evidence of Tula virus in Microtus obscurus in the region of Yili, Xinjiang, China |
title_full | Molecular evidence of Tula virus in Microtus obscurus in the region of Yili, Xinjiang, China |
title_fullStr | Molecular evidence of Tula virus in Microtus obscurus in the region of Yili, Xinjiang, China |
title_full_unstemmed | Molecular evidence of Tula virus in Microtus obscurus in the region of Yili, Xinjiang, China |
title_short | Molecular evidence of Tula virus in Microtus obscurus in the region of Yili, Xinjiang, China |
title_sort | molecular evidence of tula virus in microtus obscurus in the region of yili, xinjiang, china |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6570900/ https://www.ncbi.nlm.nih.gov/pubmed/31200661 http://dx.doi.org/10.1186/s12879-019-4133-x |
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