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In vitro induction of odontogenic activity of human dental pulp stem cells by white Portland cement enriched with zirconium oxide and zinc oxide components
Background. The aim of this in vitro study was to investigate the effect of zinc oxide (ZnO) and zirconium oxide (ZrO(2)) microparticles (MPs) and nanoparticles (NPs) in combination with white Portland cement (WPC) on odontogenic capacity of human dental pulp stem cells over a period of 21 days. Met...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Tabriz University of Medical Sciences
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6571518/ https://www.ncbi.nlm.nih.gov/pubmed/31217912 http://dx.doi.org/10.15171/joddd.2019.001 |
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author | Rahimi, Saeed Salarinasab, Sadegh Ghasemi, Negin Rahbarghazi, Reza Shahi, Shahriar Salem milani, Amin Divband, Baharak Davoudi, Paria |
author_facet | Rahimi, Saeed Salarinasab, Sadegh Ghasemi, Negin Rahbarghazi, Reza Shahi, Shahriar Salem milani, Amin Divband, Baharak Davoudi, Paria |
author_sort | Rahimi, Saeed |
collection | PubMed |
description | Background. The aim of this in vitro study was to investigate the effect of zinc oxide (ZnO) and zirconium oxide (ZrO(2)) microparticles (MPs) and nanoparticles (NPs) in combination with white Portland cement (WPC) on odontogenic capacity of human dental pulp stem cells over a period of 21 days. Methods. Synthesized ZnO and ZrO(2) particles were characterized using scanning electron microscopy and transmission electron microscopy. The viability of human dental pulp stem cells was measured by a 3-(4,5-dimethylthiazolyl-2-yl)-2,5- diphenyltetrazolium bromide assay at 7-, 14- and 21-day intervals after seeding on WPC disks enriched with ZnO and ZrO(2) MPs and NPs. Odontogenic potential of ZnO and ZrO(2) particles in combination with WPC was investigated by alkaline phosphatase (ALP) activity and ionized calcium level of supernatant culture media at different time intervals. Data were analyzed using one-way ANOVA and post hoc Tukey tests. Results. All the materials exhibited cell viability over a 21-day period, except for WPC with ZnO NPs on day 7, although it was not statistically significant (P>0.05). The ALP activity and ionized calcium level increased in all the groups compared to the control group (P<0.05). ZnO NPs had superior effect on odontogenic activity and calcium ion release compared to ZnO MPs (P=0.046). There was no significant difference between ZrO(2) MPs and NPs in odontogenic activity (P>0.05). Conclusion. WPC enriched with ZnO and ZrO(2) increased ALP activity and calcium ion release of human dental pulp stem cells over a period of 21 days in vitro. |
format | Online Article Text |
id | pubmed-6571518 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Tabriz University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-65715182019-06-19 In vitro induction of odontogenic activity of human dental pulp stem cells by white Portland cement enriched with zirconium oxide and zinc oxide components Rahimi, Saeed Salarinasab, Sadegh Ghasemi, Negin Rahbarghazi, Reza Shahi, Shahriar Salem milani, Amin Divband, Baharak Davoudi, Paria J Dent Res Dent Clin Dent Prospects Original Article Background. The aim of this in vitro study was to investigate the effect of zinc oxide (ZnO) and zirconium oxide (ZrO(2)) microparticles (MPs) and nanoparticles (NPs) in combination with white Portland cement (WPC) on odontogenic capacity of human dental pulp stem cells over a period of 21 days. Methods. Synthesized ZnO and ZrO(2) particles were characterized using scanning electron microscopy and transmission electron microscopy. The viability of human dental pulp stem cells was measured by a 3-(4,5-dimethylthiazolyl-2-yl)-2,5- diphenyltetrazolium bromide assay at 7-, 14- and 21-day intervals after seeding on WPC disks enriched with ZnO and ZrO(2) MPs and NPs. Odontogenic potential of ZnO and ZrO(2) particles in combination with WPC was investigated by alkaline phosphatase (ALP) activity and ionized calcium level of supernatant culture media at different time intervals. Data were analyzed using one-way ANOVA and post hoc Tukey tests. Results. All the materials exhibited cell viability over a 21-day period, except for WPC with ZnO NPs on day 7, although it was not statistically significant (P>0.05). The ALP activity and ionized calcium level increased in all the groups compared to the control group (P<0.05). ZnO NPs had superior effect on odontogenic activity and calcium ion release compared to ZnO MPs (P=0.046). There was no significant difference between ZrO(2) MPs and NPs in odontogenic activity (P>0.05). Conclusion. WPC enriched with ZnO and ZrO(2) increased ALP activity and calcium ion release of human dental pulp stem cells over a period of 21 days in vitro. Tabriz University of Medical Sciences 2019 2019-04-24 /pmc/articles/PMC6571518/ /pubmed/31217912 http://dx.doi.org/10.15171/joddd.2019.001 Text en © 2019 Rahimi et al. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article published and distributed by Tabriz University of Medical Sciences under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Rahimi, Saeed Salarinasab, Sadegh Ghasemi, Negin Rahbarghazi, Reza Shahi, Shahriar Salem milani, Amin Divband, Baharak Davoudi, Paria In vitro induction of odontogenic activity of human dental pulp stem cells by white Portland cement enriched with zirconium oxide and zinc oxide components |
title | In vitro induction of odontogenic activity of human dental pulp stem cells by white Portland cement enriched with zirconium oxide and zinc oxide components |
title_full | In vitro induction of odontogenic activity of human dental pulp stem cells by white Portland cement enriched with zirconium oxide and zinc oxide components |
title_fullStr | In vitro induction of odontogenic activity of human dental pulp stem cells by white Portland cement enriched with zirconium oxide and zinc oxide components |
title_full_unstemmed | In vitro induction of odontogenic activity of human dental pulp stem cells by white Portland cement enriched with zirconium oxide and zinc oxide components |
title_short | In vitro induction of odontogenic activity of human dental pulp stem cells by white Portland cement enriched with zirconium oxide and zinc oxide components |
title_sort | in vitro induction of odontogenic activity of human dental pulp stem cells by white portland cement enriched with zirconium oxide and zinc oxide components |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6571518/ https://www.ncbi.nlm.nih.gov/pubmed/31217912 http://dx.doi.org/10.15171/joddd.2019.001 |
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