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Lysosomal Acid Lipase as a Molecular Target of the Very Low Carbohydrate Ketogenic Diet in Morbidly Obese Patients: The Potential Effects on Liver Steatosis and Cardiovascular Risk Factors

A very low carbohydrate ketogenic diet (VLCKD) is an emerging technique to induce a significant, well-tolerated, and rapid loss of body weight in morbidly obese patients. The low activity of lysosomal acid lipase (LAL) could be involved in the pathogenesis of non-alcoholic fatty liver disease (NAFLD...

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Autores principales: Ministrini, Stefano, Calzini, Lucia, Nulli Migliola, Elisa, Ricci, Maria Anastasia, Roscini, Anna Rita, Siepi, Donatella, Tozzi, Giulia, Daviddi, Giulia, Martorelli, Eva-Edvige, Paganelli, Maria Teresa, Lupattelli, Graziana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6571559/
https://www.ncbi.nlm.nih.gov/pubmed/31067824
http://dx.doi.org/10.3390/jcm8050621
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author Ministrini, Stefano
Calzini, Lucia
Nulli Migliola, Elisa
Ricci, Maria Anastasia
Roscini, Anna Rita
Siepi, Donatella
Tozzi, Giulia
Daviddi, Giulia
Martorelli, Eva-Edvige
Paganelli, Maria Teresa
Lupattelli, Graziana
author_facet Ministrini, Stefano
Calzini, Lucia
Nulli Migliola, Elisa
Ricci, Maria Anastasia
Roscini, Anna Rita
Siepi, Donatella
Tozzi, Giulia
Daviddi, Giulia
Martorelli, Eva-Edvige
Paganelli, Maria Teresa
Lupattelli, Graziana
author_sort Ministrini, Stefano
collection PubMed
description A very low carbohydrate ketogenic diet (VLCKD) is an emerging technique to induce a significant, well-tolerated, and rapid loss of body weight in morbidly obese patients. The low activity of lysosomal acid lipase (LAL) could be involved in the pathogenesis of non-alcoholic fatty liver disease (NAFLD), which is a common feature in morbidly obese patients. Fifty-two obese patients suitable for a bariatric surgery intervention underwent a 25-day-long VLCKD. The biochemical markers of glucose and lipid metabolism, and flow-mediated dilation (FMD) of the brachial artery were measured before and after VLCKD. LAL activity was measured using the dried blood spot technique in 20 obese patients and in a control group of 20 healthy, normal-weight subjects. After VLCKD, we observed a significant reduction in body mass index, fasting glucose, insulinemia, and lipid profile parameters. No significant variation in FMD was observed. The number of patients with severe liver steatosis significantly decreased. LAL activity significantly increased, although the levels were not significantly different as compared to the control group. In conclusion, VLCKD induces the activity of LAL in morbidly obese subjects and reduces the secretion of all circulating lipoproteins. These effects could be attributed to the peculiar composition of the diet, which is particularly poor in carbohydrates and relatively rich in proteins.
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spelling pubmed-65715592019-06-18 Lysosomal Acid Lipase as a Molecular Target of the Very Low Carbohydrate Ketogenic Diet in Morbidly Obese Patients: The Potential Effects on Liver Steatosis and Cardiovascular Risk Factors Ministrini, Stefano Calzini, Lucia Nulli Migliola, Elisa Ricci, Maria Anastasia Roscini, Anna Rita Siepi, Donatella Tozzi, Giulia Daviddi, Giulia Martorelli, Eva-Edvige Paganelli, Maria Teresa Lupattelli, Graziana J Clin Med Article A very low carbohydrate ketogenic diet (VLCKD) is an emerging technique to induce a significant, well-tolerated, and rapid loss of body weight in morbidly obese patients. The low activity of lysosomal acid lipase (LAL) could be involved in the pathogenesis of non-alcoholic fatty liver disease (NAFLD), which is a common feature in morbidly obese patients. Fifty-two obese patients suitable for a bariatric surgery intervention underwent a 25-day-long VLCKD. The biochemical markers of glucose and lipid metabolism, and flow-mediated dilation (FMD) of the brachial artery were measured before and after VLCKD. LAL activity was measured using the dried blood spot technique in 20 obese patients and in a control group of 20 healthy, normal-weight subjects. After VLCKD, we observed a significant reduction in body mass index, fasting glucose, insulinemia, and lipid profile parameters. No significant variation in FMD was observed. The number of patients with severe liver steatosis significantly decreased. LAL activity significantly increased, although the levels were not significantly different as compared to the control group. In conclusion, VLCKD induces the activity of LAL in morbidly obese subjects and reduces the secretion of all circulating lipoproteins. These effects could be attributed to the peculiar composition of the diet, which is particularly poor in carbohydrates and relatively rich in proteins. MDPI 2019-05-07 /pmc/articles/PMC6571559/ /pubmed/31067824 http://dx.doi.org/10.3390/jcm8050621 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ministrini, Stefano
Calzini, Lucia
Nulli Migliola, Elisa
Ricci, Maria Anastasia
Roscini, Anna Rita
Siepi, Donatella
Tozzi, Giulia
Daviddi, Giulia
Martorelli, Eva-Edvige
Paganelli, Maria Teresa
Lupattelli, Graziana
Lysosomal Acid Lipase as a Molecular Target of the Very Low Carbohydrate Ketogenic Diet in Morbidly Obese Patients: The Potential Effects on Liver Steatosis and Cardiovascular Risk Factors
title Lysosomal Acid Lipase as a Molecular Target of the Very Low Carbohydrate Ketogenic Diet in Morbidly Obese Patients: The Potential Effects on Liver Steatosis and Cardiovascular Risk Factors
title_full Lysosomal Acid Lipase as a Molecular Target of the Very Low Carbohydrate Ketogenic Diet in Morbidly Obese Patients: The Potential Effects on Liver Steatosis and Cardiovascular Risk Factors
title_fullStr Lysosomal Acid Lipase as a Molecular Target of the Very Low Carbohydrate Ketogenic Diet in Morbidly Obese Patients: The Potential Effects on Liver Steatosis and Cardiovascular Risk Factors
title_full_unstemmed Lysosomal Acid Lipase as a Molecular Target of the Very Low Carbohydrate Ketogenic Diet in Morbidly Obese Patients: The Potential Effects on Liver Steatosis and Cardiovascular Risk Factors
title_short Lysosomal Acid Lipase as a Molecular Target of the Very Low Carbohydrate Ketogenic Diet in Morbidly Obese Patients: The Potential Effects on Liver Steatosis and Cardiovascular Risk Factors
title_sort lysosomal acid lipase as a molecular target of the very low carbohydrate ketogenic diet in morbidly obese patients: the potential effects on liver steatosis and cardiovascular risk factors
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6571559/
https://www.ncbi.nlm.nih.gov/pubmed/31067824
http://dx.doi.org/10.3390/jcm8050621
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