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Clitoria ternatea Flower Petal Extract Inhibits Adipogenesis and Lipid Accumulation in 3T3-L1 Preadipocytes by Downregulating Adipogenic Gene Expression

Clitoria ternatea (commonly known as blue pea) flower petal extract (CTE) is used as a natural colorant in a variety of foods and beverages. The objective of study was to determine the inhibitory effect of CTE on adipogenesis in 3T3-L1 preadipocytes. The phytochemical profiles of CTE were analyzed b...

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Autores principales: Chayaratanasin, Poramin, Caobi, Allen, Suparpprom, Chaturong, Saenset, Sudarat, Pasukamonset, Porntip, Suanpairintr, Nipattra, Barbieri, Manuel Alejandro, Adisakwattana, Sirichai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6571662/
https://www.ncbi.nlm.nih.gov/pubmed/31108834
http://dx.doi.org/10.3390/molecules24101894
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author Chayaratanasin, Poramin
Caobi, Allen
Suparpprom, Chaturong
Saenset, Sudarat
Pasukamonset, Porntip
Suanpairintr, Nipattra
Barbieri, Manuel Alejandro
Adisakwattana, Sirichai
author_facet Chayaratanasin, Poramin
Caobi, Allen
Suparpprom, Chaturong
Saenset, Sudarat
Pasukamonset, Porntip
Suanpairintr, Nipattra
Barbieri, Manuel Alejandro
Adisakwattana, Sirichai
author_sort Chayaratanasin, Poramin
collection PubMed
description Clitoria ternatea (commonly known as blue pea) flower petal extract (CTE) is used as a natural colorant in a variety of foods and beverages. The objective of study was to determine the inhibitory effect of CTE on adipogenesis in 3T3-L1 preadipocytes. The phytochemical profiles of CTE were analyzed by liquid chromatography and tandem mass spectrometry (LC-MS/MS). Anti-adipogenesis effect of CTE was measured by using Oil Red O staining, intracellular triglyceride assay, quantitative real-time PCR and western blot analysis in 3T3-L1 adipocytes. Cell cycle studies were performed by flow cytometry. Lipolysis experiments were performed using a colorimetric assay kit. In early stages, CTE demonstrated anti-adipogenic effects through inhibition of proliferation and cell cycle retardation by suppressing expression of phospho-Akt and phospho-ERK1/2 signaling pathway. The results also showed that CTE inhibited the late stage of differentiation through diminishing expression of adipogenic transcription factors including PPARγ and C/EBPα. The inhibitory action was subsequently attenuated in downregulation of fatty acid synthase and acetyl-CoA carboxylase, causing the reduction of TG accumulation. In addition, CTE also enhanced catecholamine-induced lipolysis in adipocytes. These results suggest that CTE effectively attenuates adipogenesis by controlling cell cycle progression and downregulating adipogenic gene expression.
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spelling pubmed-65716622019-06-18 Clitoria ternatea Flower Petal Extract Inhibits Adipogenesis and Lipid Accumulation in 3T3-L1 Preadipocytes by Downregulating Adipogenic Gene Expression Chayaratanasin, Poramin Caobi, Allen Suparpprom, Chaturong Saenset, Sudarat Pasukamonset, Porntip Suanpairintr, Nipattra Barbieri, Manuel Alejandro Adisakwattana, Sirichai Molecules Article Clitoria ternatea (commonly known as blue pea) flower petal extract (CTE) is used as a natural colorant in a variety of foods and beverages. The objective of study was to determine the inhibitory effect of CTE on adipogenesis in 3T3-L1 preadipocytes. The phytochemical profiles of CTE were analyzed by liquid chromatography and tandem mass spectrometry (LC-MS/MS). Anti-adipogenesis effect of CTE was measured by using Oil Red O staining, intracellular triglyceride assay, quantitative real-time PCR and western blot analysis in 3T3-L1 adipocytes. Cell cycle studies were performed by flow cytometry. Lipolysis experiments were performed using a colorimetric assay kit. In early stages, CTE demonstrated anti-adipogenic effects through inhibition of proliferation and cell cycle retardation by suppressing expression of phospho-Akt and phospho-ERK1/2 signaling pathway. The results also showed that CTE inhibited the late stage of differentiation through diminishing expression of adipogenic transcription factors including PPARγ and C/EBPα. The inhibitory action was subsequently attenuated in downregulation of fatty acid synthase and acetyl-CoA carboxylase, causing the reduction of TG accumulation. In addition, CTE also enhanced catecholamine-induced lipolysis in adipocytes. These results suggest that CTE effectively attenuates adipogenesis by controlling cell cycle progression and downregulating adipogenic gene expression. MDPI 2019-05-17 /pmc/articles/PMC6571662/ /pubmed/31108834 http://dx.doi.org/10.3390/molecules24101894 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chayaratanasin, Poramin
Caobi, Allen
Suparpprom, Chaturong
Saenset, Sudarat
Pasukamonset, Porntip
Suanpairintr, Nipattra
Barbieri, Manuel Alejandro
Adisakwattana, Sirichai
Clitoria ternatea Flower Petal Extract Inhibits Adipogenesis and Lipid Accumulation in 3T3-L1 Preadipocytes by Downregulating Adipogenic Gene Expression
title Clitoria ternatea Flower Petal Extract Inhibits Adipogenesis and Lipid Accumulation in 3T3-L1 Preadipocytes by Downregulating Adipogenic Gene Expression
title_full Clitoria ternatea Flower Petal Extract Inhibits Adipogenesis and Lipid Accumulation in 3T3-L1 Preadipocytes by Downregulating Adipogenic Gene Expression
title_fullStr Clitoria ternatea Flower Petal Extract Inhibits Adipogenesis and Lipid Accumulation in 3T3-L1 Preadipocytes by Downregulating Adipogenic Gene Expression
title_full_unstemmed Clitoria ternatea Flower Petal Extract Inhibits Adipogenesis and Lipid Accumulation in 3T3-L1 Preadipocytes by Downregulating Adipogenic Gene Expression
title_short Clitoria ternatea Flower Petal Extract Inhibits Adipogenesis and Lipid Accumulation in 3T3-L1 Preadipocytes by Downregulating Adipogenic Gene Expression
title_sort clitoria ternatea flower petal extract inhibits adipogenesis and lipid accumulation in 3t3-l1 preadipocytes by downregulating adipogenic gene expression
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6571662/
https://www.ncbi.nlm.nih.gov/pubmed/31108834
http://dx.doi.org/10.3390/molecules24101894
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