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Maternal High Fat Diet and in-Utero Metformin Exposure Significantly Impact upon the Fetal Renal Proteome of Male Mice
There is accumulating evidence for fetal programming of later kidney disease by maternal obesity or associated conditions. We performed a hypothesis-generating study to identify potentially underlying mechanisms. Female mice were randomly split in two groups and fed either a standard diet (SD) or hi...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6571731/ https://www.ncbi.nlm.nih.gov/pubmed/31083566 http://dx.doi.org/10.3390/jcm8050663 |
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author | Nüsken, Eva Turnwald, Eva-Maria Fink, Gregor Voggel, Jenny Yosy, Christopher Kretschmer, Tobias Handwerk, Marion Wohlfarth, Maria Weber, Lutz T. Hucklenbruch-Rother, Eva Dötsch, Jörg Nüsken, Kai-Dietrich Appel, Sarah |
author_facet | Nüsken, Eva Turnwald, Eva-Maria Fink, Gregor Voggel, Jenny Yosy, Christopher Kretschmer, Tobias Handwerk, Marion Wohlfarth, Maria Weber, Lutz T. Hucklenbruch-Rother, Eva Dötsch, Jörg Nüsken, Kai-Dietrich Appel, Sarah |
author_sort | Nüsken, Eva |
collection | PubMed |
description | There is accumulating evidence for fetal programming of later kidney disease by maternal obesity or associated conditions. We performed a hypothesis-generating study to identify potentially underlying mechanisms. Female mice were randomly split in two groups and fed either a standard diet (SD) or high fat diet (HFD) from weaning until mating and during pregnancy. Half of the dams from both groups were treated with metformin ((M), 380 mg/kg), resulting in four experimental groups (SD, SD-M, HFD, HFD-M). Caesarean section was performed on gestational day 18.5. Fetal kidney tissue was isolated from cryo-slices using laser microdissection methods and a proteomic screen was performed. For single proteins, a fold change ≥1.5 and q-value <0.05 were considered to be statistically significant. Interestingly, HFD versus SD had a larger effect on the proteome of fetal kidneys (56 proteins affected; interaction clusters shown for proteins concerning transcription/translation, mitochondrial processes, eicosanoid metabolism, H2S-synthesis and membrane remodeling) than metformin exposure in either SD (29 proteins affected; clusters shown for proteins involved in transcription/translation) or HFD (6 proteins affected; no cluster). By further analysis, ATP6V1G1, THY1, PRKCA and NDUFB3 were identified as the most promising candidates potentially mediating reprogramming effects of metformin in a maternal high fat diet. |
format | Online Article Text |
id | pubmed-6571731 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-65717312019-06-18 Maternal High Fat Diet and in-Utero Metformin Exposure Significantly Impact upon the Fetal Renal Proteome of Male Mice Nüsken, Eva Turnwald, Eva-Maria Fink, Gregor Voggel, Jenny Yosy, Christopher Kretschmer, Tobias Handwerk, Marion Wohlfarth, Maria Weber, Lutz T. Hucklenbruch-Rother, Eva Dötsch, Jörg Nüsken, Kai-Dietrich Appel, Sarah J Clin Med Article There is accumulating evidence for fetal programming of later kidney disease by maternal obesity or associated conditions. We performed a hypothesis-generating study to identify potentially underlying mechanisms. Female mice were randomly split in two groups and fed either a standard diet (SD) or high fat diet (HFD) from weaning until mating and during pregnancy. Half of the dams from both groups were treated with metformin ((M), 380 mg/kg), resulting in four experimental groups (SD, SD-M, HFD, HFD-M). Caesarean section was performed on gestational day 18.5. Fetal kidney tissue was isolated from cryo-slices using laser microdissection methods and a proteomic screen was performed. For single proteins, a fold change ≥1.5 and q-value <0.05 were considered to be statistically significant. Interestingly, HFD versus SD had a larger effect on the proteome of fetal kidneys (56 proteins affected; interaction clusters shown for proteins concerning transcription/translation, mitochondrial processes, eicosanoid metabolism, H2S-synthesis and membrane remodeling) than metformin exposure in either SD (29 proteins affected; clusters shown for proteins involved in transcription/translation) or HFD (6 proteins affected; no cluster). By further analysis, ATP6V1G1, THY1, PRKCA and NDUFB3 were identified as the most promising candidates potentially mediating reprogramming effects of metformin in a maternal high fat diet. MDPI 2019-05-11 /pmc/articles/PMC6571731/ /pubmed/31083566 http://dx.doi.org/10.3390/jcm8050663 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Nüsken, Eva Turnwald, Eva-Maria Fink, Gregor Voggel, Jenny Yosy, Christopher Kretschmer, Tobias Handwerk, Marion Wohlfarth, Maria Weber, Lutz T. Hucklenbruch-Rother, Eva Dötsch, Jörg Nüsken, Kai-Dietrich Appel, Sarah Maternal High Fat Diet and in-Utero Metformin Exposure Significantly Impact upon the Fetal Renal Proteome of Male Mice |
title | Maternal High Fat Diet and in-Utero Metformin Exposure Significantly Impact upon the Fetal Renal Proteome of Male Mice |
title_full | Maternal High Fat Diet and in-Utero Metformin Exposure Significantly Impact upon the Fetal Renal Proteome of Male Mice |
title_fullStr | Maternal High Fat Diet and in-Utero Metformin Exposure Significantly Impact upon the Fetal Renal Proteome of Male Mice |
title_full_unstemmed | Maternal High Fat Diet and in-Utero Metformin Exposure Significantly Impact upon the Fetal Renal Proteome of Male Mice |
title_short | Maternal High Fat Diet and in-Utero Metformin Exposure Significantly Impact upon the Fetal Renal Proteome of Male Mice |
title_sort | maternal high fat diet and in-utero metformin exposure significantly impact upon the fetal renal proteome of male mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6571731/ https://www.ncbi.nlm.nih.gov/pubmed/31083566 http://dx.doi.org/10.3390/jcm8050663 |
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