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Computational Simulation of Adapter Length-Dependent LASSO Probe Capture Efficiency

Multiplexed cloning of long DNA sequences is a valuable technique in many biotechnology applications, such as long-read genome sequencing and the creation of open reading frame (ORF) libraries. Long-adapter single-stranded oligonucleotide (LASSO) probes have shown promise as a tool to clone long DNA...

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Detalles Bibliográficos
Autores principales: Liu, Jingqian, Shukor, Syukri, Li, Shuxiang, Tamayo, Alfred, Tosi, Lorenzo, Larman, Benjamin, Nanda, Vikas, Olson, Wilma K., Parekkadan, Biju
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6571849/
https://www.ncbi.nlm.nih.gov/pubmed/31121947
http://dx.doi.org/10.3390/biom9050199