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Non-cultivated Cotton Species (Gossypium spp.) Act as a Reservoir for Cotton Leaf Curl Begomoviruses and Associated Satellites

A collection of cultivated and non-cultivated species of cotton (Gossypium spp.) has been maintained for the last four decades in Multan, Pakistan. This geographical location has been observed as a hotspot for the evolution of begomoviruses and satellites associated with cotton leaf curl disease (CL...

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Autores principales: Shakir, Sara, Zaidi, Syed Shan-e-Ali, Atiq-ur-Rehman, Farooq, Muhammad, Amin, Imran, Scheffler, Jodi, Scheffler, Brian, Shah Nawaz-ul-Rehman, Muhammad, Mansoor, Shahid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6571856/
https://www.ncbi.nlm.nih.gov/pubmed/31091727
http://dx.doi.org/10.3390/plants8050127
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author Shakir, Sara
Zaidi, Syed Shan-e-Ali
Atiq-ur-Rehman,
Farooq, Muhammad
Amin, Imran
Scheffler, Jodi
Scheffler, Brian
Shah Nawaz-ul-Rehman, Muhammad
Mansoor, Shahid
author_facet Shakir, Sara
Zaidi, Syed Shan-e-Ali
Atiq-ur-Rehman,
Farooq, Muhammad
Amin, Imran
Scheffler, Jodi
Scheffler, Brian
Shah Nawaz-ul-Rehman, Muhammad
Mansoor, Shahid
author_sort Shakir, Sara
collection PubMed
description A collection of cultivated and non-cultivated species of cotton (Gossypium spp.) has been maintained for the last four decades in Multan, Pakistan. This geographical location has been observed as a hotspot for the evolution of begomoviruses and satellites associated with cotton leaf curl disease (CLCuD). Recent studies showed that begomoviruses responsible for the CLCuD epidemic in the 1990s, and that almost disappeared from the CLCuD complex in 2000s, have been observed again in CLCuD-infected cotton fields. To identify host species that acted as probable reservoirs for these viruses, we characterized begomoviruses and satellites in non-cultivated cotton species G. raimondii, G. thurberi and G. mustelinum and identified several species of CLCuD associated begomoviruses and satellites. Further, phylogenetic analysis indicated that the identified begomoviruses and beta/alphasatellites are closely related to the ones associated with the most recent CLCuD complex. qPCR indicated that the comparative level of virus significantly decreased in the presence of alphasatellites. Our results indicated that non-cultivated cotton species have been continuously challenged by diverse begomoviruses and associated satellites and act as reservoirs for CLCuD associated begomoviruses. These results provide novel insights into understanding the spread of begomoviruses and associated satellites in New World cotton species introduced into the Old World.
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spelling pubmed-65718562019-06-18 Non-cultivated Cotton Species (Gossypium spp.) Act as a Reservoir for Cotton Leaf Curl Begomoviruses and Associated Satellites Shakir, Sara Zaidi, Syed Shan-e-Ali Atiq-ur-Rehman, Farooq, Muhammad Amin, Imran Scheffler, Jodi Scheffler, Brian Shah Nawaz-ul-Rehman, Muhammad Mansoor, Shahid Plants (Basel) Article A collection of cultivated and non-cultivated species of cotton (Gossypium spp.) has been maintained for the last four decades in Multan, Pakistan. This geographical location has been observed as a hotspot for the evolution of begomoviruses and satellites associated with cotton leaf curl disease (CLCuD). Recent studies showed that begomoviruses responsible for the CLCuD epidemic in the 1990s, and that almost disappeared from the CLCuD complex in 2000s, have been observed again in CLCuD-infected cotton fields. To identify host species that acted as probable reservoirs for these viruses, we characterized begomoviruses and satellites in non-cultivated cotton species G. raimondii, G. thurberi and G. mustelinum and identified several species of CLCuD associated begomoviruses and satellites. Further, phylogenetic analysis indicated that the identified begomoviruses and beta/alphasatellites are closely related to the ones associated with the most recent CLCuD complex. qPCR indicated that the comparative level of virus significantly decreased in the presence of alphasatellites. Our results indicated that non-cultivated cotton species have been continuously challenged by diverse begomoviruses and associated satellites and act as reservoirs for CLCuD associated begomoviruses. These results provide novel insights into understanding the spread of begomoviruses and associated satellites in New World cotton species introduced into the Old World. MDPI 2019-05-14 /pmc/articles/PMC6571856/ /pubmed/31091727 http://dx.doi.org/10.3390/plants8050127 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shakir, Sara
Zaidi, Syed Shan-e-Ali
Atiq-ur-Rehman,
Farooq, Muhammad
Amin, Imran
Scheffler, Jodi
Scheffler, Brian
Shah Nawaz-ul-Rehman, Muhammad
Mansoor, Shahid
Non-cultivated Cotton Species (Gossypium spp.) Act as a Reservoir for Cotton Leaf Curl Begomoviruses and Associated Satellites
title Non-cultivated Cotton Species (Gossypium spp.) Act as a Reservoir for Cotton Leaf Curl Begomoviruses and Associated Satellites
title_full Non-cultivated Cotton Species (Gossypium spp.) Act as a Reservoir for Cotton Leaf Curl Begomoviruses and Associated Satellites
title_fullStr Non-cultivated Cotton Species (Gossypium spp.) Act as a Reservoir for Cotton Leaf Curl Begomoviruses and Associated Satellites
title_full_unstemmed Non-cultivated Cotton Species (Gossypium spp.) Act as a Reservoir for Cotton Leaf Curl Begomoviruses and Associated Satellites
title_short Non-cultivated Cotton Species (Gossypium spp.) Act as a Reservoir for Cotton Leaf Curl Begomoviruses and Associated Satellites
title_sort non-cultivated cotton species (gossypium spp.) act as a reservoir for cotton leaf curl begomoviruses and associated satellites
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6571856/
https://www.ncbi.nlm.nih.gov/pubmed/31091727
http://dx.doi.org/10.3390/plants8050127
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