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Peptide Mapping, In Silico and In Vivo Analysis of Allergenic Sorghum Profilin Peptides

Background and objectives: Nearly 20–30% of the world’s population suffers from allergic rhinitis, among them 15% are progressing to asthma conditions. Sorghum bicolor profilin (Sorb PF), one of the panallergens, was identified, but the allergen specificity is not yet characterized. Materials and Me...

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Autores principales: Bokka, Chandra Sekhar, Veeramachaneni, Ganesh Kumar, Thunuguntla, V. B. S. C., Bobbillapati, Janakiram, Bondili, Jayakumar Singh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6571906/
https://www.ncbi.nlm.nih.gov/pubmed/31117233
http://dx.doi.org/10.3390/medicina55050178
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author Bokka, Chandra Sekhar
Veeramachaneni, Ganesh Kumar
Thunuguntla, V. B. S. C.
Bobbillapati, Janakiram
Bondili, Jayakumar Singh
author_facet Bokka, Chandra Sekhar
Veeramachaneni, Ganesh Kumar
Thunuguntla, V. B. S. C.
Bobbillapati, Janakiram
Bondili, Jayakumar Singh
author_sort Bokka, Chandra Sekhar
collection PubMed
description Background and objectives: Nearly 20–30% of the world’s population suffers from allergic rhinitis, among them 15% are progressing to asthma conditions. Sorghum bicolor profilin (Sorb PF), one of the panallergens, was identified, but the allergen specificity is not yet characterized. Materials and Methods: To map the antigenic determinants responsible for IgE binding, the present study is focused on in silico modeling, simulation of Sorb PF and docking of the Sorb PF peptides (PF1-6) against IgG and IgE, followed by in vivo evaluation of the peptides for its allergenicity in mice. Results: Peptide PF3 and PF4 displayed high docking G-scores (−9.05) against IgE only. The mice sensitized with PF3 peptide showed increased levels of IL5, IL12, TNF-alpha, and GMCSF when compared to other peptides and controls, signifying a strong, Th2-based response. Concurrently, the Th1 pathway was inhibited by low levels of cytokine IL2, IFN-γ, and IL-10 justifying the role of PF3 in allergenic IgE response. Conclusions: Based on the results of overlapping peptides PF3 and PF4, the N-terminal part of the PF3 peptide (TGQALVI) plays a crucial role in allergenic response of Sorghum profilin.
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spelling pubmed-65719062019-06-18 Peptide Mapping, In Silico and In Vivo Analysis of Allergenic Sorghum Profilin Peptides Bokka, Chandra Sekhar Veeramachaneni, Ganesh Kumar Thunuguntla, V. B. S. C. Bobbillapati, Janakiram Bondili, Jayakumar Singh Medicina (Kaunas) Article Background and objectives: Nearly 20–30% of the world’s population suffers from allergic rhinitis, among them 15% are progressing to asthma conditions. Sorghum bicolor profilin (Sorb PF), one of the panallergens, was identified, but the allergen specificity is not yet characterized. Materials and Methods: To map the antigenic determinants responsible for IgE binding, the present study is focused on in silico modeling, simulation of Sorb PF and docking of the Sorb PF peptides (PF1-6) against IgG and IgE, followed by in vivo evaluation of the peptides for its allergenicity in mice. Results: Peptide PF3 and PF4 displayed high docking G-scores (−9.05) against IgE only. The mice sensitized with PF3 peptide showed increased levels of IL5, IL12, TNF-alpha, and GMCSF when compared to other peptides and controls, signifying a strong, Th2-based response. Concurrently, the Th1 pathway was inhibited by low levels of cytokine IL2, IFN-γ, and IL-10 justifying the role of PF3 in allergenic IgE response. Conclusions: Based on the results of overlapping peptides PF3 and PF4, the N-terminal part of the PF3 peptide (TGQALVI) plays a crucial role in allergenic response of Sorghum profilin. MDPI 2019-05-21 /pmc/articles/PMC6571906/ /pubmed/31117233 http://dx.doi.org/10.3390/medicina55050178 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bokka, Chandra Sekhar
Veeramachaneni, Ganesh Kumar
Thunuguntla, V. B. S. C.
Bobbillapati, Janakiram
Bondili, Jayakumar Singh
Peptide Mapping, In Silico and In Vivo Analysis of Allergenic Sorghum Profilin Peptides
title Peptide Mapping, In Silico and In Vivo Analysis of Allergenic Sorghum Profilin Peptides
title_full Peptide Mapping, In Silico and In Vivo Analysis of Allergenic Sorghum Profilin Peptides
title_fullStr Peptide Mapping, In Silico and In Vivo Analysis of Allergenic Sorghum Profilin Peptides
title_full_unstemmed Peptide Mapping, In Silico and In Vivo Analysis of Allergenic Sorghum Profilin Peptides
title_short Peptide Mapping, In Silico and In Vivo Analysis of Allergenic Sorghum Profilin Peptides
title_sort peptide mapping, in silico and in vivo analysis of allergenic sorghum profilin peptides
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6571906/
https://www.ncbi.nlm.nih.gov/pubmed/31117233
http://dx.doi.org/10.3390/medicina55050178
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