The Difference in Cytotoxic Activity between Two Optical Isomers of Gelsemine from Gelsemium elegans Benth. on PC12 Cells

Two optical isomers, +/− gelsemine (1, 2), together with one known compound were isolated from the whole plant of G. elegans. The structures of the separated constituents were elucidated on 1D and 2D ((1)H-(1)H COSY, HMBC, HSQC) NMR spectroscopy and high-resolution mass spectrometry (HRMS). The isol...

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Detalles Bibliográficos
Autores principales: Lin, Li, Liu, Yan-Chun, Liu, Zhao-Ying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6571982/
https://www.ncbi.nlm.nih.gov/pubmed/31130596
http://dx.doi.org/10.3390/molecules24102004
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author Lin, Li
Liu, Yan-Chun
Liu, Zhao-Ying
author_facet Lin, Li
Liu, Yan-Chun
Liu, Zhao-Ying
author_sort Lin, Li
collection PubMed
description Two optical isomers, +/− gelsemine (1, 2), together with one known compound were isolated from the whole plant of G. elegans. The structures of the separated constituents were elucidated on 1D and 2D ((1)H-(1)H COSY, HMBC, HSQC) NMR spectroscopy and high-resolution mass spectrometry (HRMS). The isolated alkaloids were tested in vitro for cytotoxic potential against PC12 cells by the MTT assay. As a result, (+) gelsemine (compound 1) exhibited cytotoxic activity against PC12 cells with an IC(50) value of 31.59 μM, while (−) gelsemine (compound 2) was not cytotoxic.
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spelling pubmed-65719822019-06-18 The Difference in Cytotoxic Activity between Two Optical Isomers of Gelsemine from Gelsemium elegans Benth. on PC12 Cells Lin, Li Liu, Yan-Chun Liu, Zhao-Ying Molecules Communication Two optical isomers, +/− gelsemine (1, 2), together with one known compound were isolated from the whole plant of G. elegans. The structures of the separated constituents were elucidated on 1D and 2D ((1)H-(1)H COSY, HMBC, HSQC) NMR spectroscopy and high-resolution mass spectrometry (HRMS). The isolated alkaloids were tested in vitro for cytotoxic potential against PC12 cells by the MTT assay. As a result, (+) gelsemine (compound 1) exhibited cytotoxic activity against PC12 cells with an IC(50) value of 31.59 μM, while (−) gelsemine (compound 2) was not cytotoxic. MDPI 2019-05-25 /pmc/articles/PMC6571982/ /pubmed/31130596 http://dx.doi.org/10.3390/molecules24102004 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Lin, Li
Liu, Yan-Chun
Liu, Zhao-Ying
The Difference in Cytotoxic Activity between Two Optical Isomers of Gelsemine from Gelsemium elegans Benth. on PC12 Cells
title The Difference in Cytotoxic Activity between Two Optical Isomers of Gelsemine from Gelsemium elegans Benth. on PC12 Cells
title_full The Difference in Cytotoxic Activity between Two Optical Isomers of Gelsemine from Gelsemium elegans Benth. on PC12 Cells
title_fullStr The Difference in Cytotoxic Activity between Two Optical Isomers of Gelsemine from Gelsemium elegans Benth. on PC12 Cells
title_full_unstemmed The Difference in Cytotoxic Activity between Two Optical Isomers of Gelsemine from Gelsemium elegans Benth. on PC12 Cells
title_short The Difference in Cytotoxic Activity between Two Optical Isomers of Gelsemine from Gelsemium elegans Benth. on PC12 Cells
title_sort difference in cytotoxic activity between two optical isomers of gelsemine from gelsemium elegans benth. on pc12 cells
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6571982/
https://www.ncbi.nlm.nih.gov/pubmed/31130596
http://dx.doi.org/10.3390/molecules24102004
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